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Bilirubin impairs intestinal regrowth following massive small bowel resection in a rat model.
J Pediatr Gastroenterol Nutr. 2009 Jul; 49(1):16-22.JP

Abstract

OBJECTIVES

The purpose of the present study was to evaluate the effects of exogenous bilirubin on structural intestinal adaptation, cell proliferation, and apoptosis in a rat model of short bowel syndrome (SBS).

MATERIALS AND METHODS

Male Sprague-Dawley rats were divided into 5 experimental groups: Sham rats underwent bowel transection and reanastomosis, sham multiple doses of bilirubin (MDB) rats underwent bowel transection and were treated with bilirubin, SBS rats underwent a 75% small bowel resection, SBS-SDB (single dose bilirubin) rats underwent a bowel resection and were treated with a single dose of bilirubin, and SBS-MDB underwent a bowel resection and were treated with 3 doses of bilirubin. Bilirubin was administered intraperitoneally from the 7th day through the 14th day postoperatively. Serum total bilirubin concentration over time was evaluated in 5 SBS-SDB rats following a single intraperitoneal dose. Total bilirubin, alanine aminotransferase, and aspartate aminotransferase in serum and parameters of intestinal adaptation, enterocyte proliferation, and enterocyte apoptosis were determined on day 15.

RESULTS

SBS-SDB and SBS-MDB animals demonstrated lower ileal bowel and mucosal weights, jejunal mucosal DNA and ileal mucosal protein, and jejunal and ileal villus height and crypt depth (vs SBS animals). Bilirubin-treated rats showed a lower apoptotic index in jejunum and ileum and a trend toward an increase in cell proliferation in jejunum and ileum (vs SBS group).

CONCLUSIONS

In a rat model of SBS, exogenous bilirubin inhibits structural intestinal adaptation. Increased cell proliferation and decreased apoptosis may be considered adaptive mechanisms that maintain cell mass.

Authors+Show Affiliations

Department of Pediatric Surgery, Bnai Zion Medical Center, 47 Golomb St, PO Box 4940, Haifa 31048, Israel. igor-dr@internet-zahav.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19465868

Citation

Sukhotnik, Igor, et al. "Bilirubin Impairs Intestinal Regrowth Following Massive Small Bowel Resection in a Rat Model." Journal of Pediatric Gastroenterology and Nutrition, vol. 49, no. 1, 2009, pp. 16-22.
Sukhotnik I, Shaoul R, Lieber M, et al. Bilirubin impairs intestinal regrowth following massive small bowel resection in a rat model. J Pediatr Gastroenterol Nutr. 2009;49(1):16-22.
Sukhotnik, I., Shaoul, R., Lieber, M., Coran, A. G., Abassi, Z., Shiloni, E., & Mogilner, J. G. (2009). Bilirubin impairs intestinal regrowth following massive small bowel resection in a rat model. Journal of Pediatric Gastroenterology and Nutrition, 49(1), 16-22. https://doi.org/10.1097/MPG.0b013e31819a4dff
Sukhotnik I, et al. Bilirubin Impairs Intestinal Regrowth Following Massive Small Bowel Resection in a Rat Model. J Pediatr Gastroenterol Nutr. 2009;49(1):16-22. PubMed PMID: 19465868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bilirubin impairs intestinal regrowth following massive small bowel resection in a rat model. AU - Sukhotnik,Igor, AU - Shaoul,Ron, AU - Lieber,Michael, AU - Coran,Arnold G, AU - Abassi,Zaid, AU - Shiloni,Eitan, AU - Mogilner,Jorge G, PY - 2009/5/26/entrez PY - 2009/5/26/pubmed PY - 2009/11/5/medline SP - 16 EP - 22 JF - Journal of pediatric gastroenterology and nutrition JO - J Pediatr Gastroenterol Nutr VL - 49 IS - 1 N2 - OBJECTIVES: The purpose of the present study was to evaluate the effects of exogenous bilirubin on structural intestinal adaptation, cell proliferation, and apoptosis in a rat model of short bowel syndrome (SBS). MATERIALS AND METHODS: Male Sprague-Dawley rats were divided into 5 experimental groups: Sham rats underwent bowel transection and reanastomosis, sham multiple doses of bilirubin (MDB) rats underwent bowel transection and were treated with bilirubin, SBS rats underwent a 75% small bowel resection, SBS-SDB (single dose bilirubin) rats underwent a bowel resection and were treated with a single dose of bilirubin, and SBS-MDB underwent a bowel resection and were treated with 3 doses of bilirubin. Bilirubin was administered intraperitoneally from the 7th day through the 14th day postoperatively. Serum total bilirubin concentration over time was evaluated in 5 SBS-SDB rats following a single intraperitoneal dose. Total bilirubin, alanine aminotransferase, and aspartate aminotransferase in serum and parameters of intestinal adaptation, enterocyte proliferation, and enterocyte apoptosis were determined on day 15. RESULTS: SBS-SDB and SBS-MDB animals demonstrated lower ileal bowel and mucosal weights, jejunal mucosal DNA and ileal mucosal protein, and jejunal and ileal villus height and crypt depth (vs SBS animals). Bilirubin-treated rats showed a lower apoptotic index in jejunum and ileum and a trend toward an increase in cell proliferation in jejunum and ileum (vs SBS group). CONCLUSIONS: In a rat model of SBS, exogenous bilirubin inhibits structural intestinal adaptation. Increased cell proliferation and decreased apoptosis may be considered adaptive mechanisms that maintain cell mass. SN - 1536-4801 UR - https://www.unboundmedicine.com/medline/citation/19465868/Bilirubin_impairs_intestinal_regrowth_following_massive_small_bowel_resection_in_a_rat_model_ L2 - https://doi.org/10.1097/MPG.0b013e31819a4dff DB - PRIME DP - Unbound Medicine ER -