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Alkaline comet assay for genotoxic effect detection in neotropical fish Prochilodus lineatus (Pisces, Curimatidae).
Bull Environ Contam Toxicol. 2009 Aug; 83(2):155-8.BE

Abstract

Toxicants on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using alkaline comet assay applied on erythrocytes after in vivo exposure of Prochilodus lineatus to different concentrations of Cypermethrin (0.300, 0.150, 0.075 and 0.000 microg/L) as a probable chemical mutagen. The results revealed a significantly higher level of DNA damage at all concentrations of Cypermethrin tested compared to control and background level (p < 0.05). We have standardized the technique for one of the most common native fish species that will be useful for biomonitoring genotoxicity in polluted waters of the region.

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Authors+Show Affiliations

Simoniello MF
Cátedra de Toxicología y Bioquímica Legal, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Paraje El Pozo C.C 242, Santa Fe, 3000, Argentina.
Gigena F
No affiliation info available
Poletta G
No affiliation info available
Loteste A
No affiliation info available
Kleinsorge E
No affiliation info available
Campana M
No affiliation info available
Scagnetti J
No affiliation info available
Parma MJ
No affiliation info available

MeSH

AlkaliesAnimalsComet AssayDNA DamageEnvironmental MonitoringFishesInsecticidesPyrethrinsWater Pollutants, Chemical

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19466374

Citation

Simoniello, M F., et al. "Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus Lineatus (Pisces, Curimatidae)." Bulletin of Environmental Contamination and Toxicology, vol. 83, no. 2, 2009, pp. 155-8.
Simoniello MF, Gigena F, Poletta G, et al. Alkaline comet assay for genotoxic effect detection in neotropical fish Prochilodus lineatus (Pisces, Curimatidae). Bull Environ Contam Toxicol. 2009;83(2):155-8.
Simoniello, M. F., Gigena, F., Poletta, G., Loteste, A., Kleinsorge, E., Campana, M., Scagnetti, J., & Parma, M. J. (2009). Alkaline comet assay for genotoxic effect detection in neotropical fish Prochilodus lineatus (Pisces, Curimatidae). Bulletin of Environmental Contamination and Toxicology, 83(2), 155-8. https://doi.org/10.1007/s00128-009-9771-z
Simoniello MF, et al. Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus Lineatus (Pisces, Curimatidae). Bull Environ Contam Toxicol. 2009;83(2):155-8. PubMed PMID: 19466374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alkaline comet assay for genotoxic effect detection in neotropical fish Prochilodus lineatus (Pisces, Curimatidae). AU - Simoniello,M F, AU - Gigena,F, AU - Poletta,G, AU - Loteste,A, AU - Kleinsorge,E, AU - Campana,M, AU - Scagnetti,J, AU - Parma,M J, Y1 - 2009/05/23/ PY - 2008/07/23/received PY - 2009/05/05/accepted PY - 2009/5/26/entrez PY - 2009/5/26/pubmed PY - 2009/8/22/medline SP - 155 EP - 8 JF - Bulletin of environmental contamination and toxicology JO - Bull Environ Contam Toxicol VL - 83 IS - 2 N2 - Toxicants on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using alkaline comet assay applied on erythrocytes after in vivo exposure of Prochilodus lineatus to different concentrations of Cypermethrin (0.300, 0.150, 0.075 and 0.000 microg/L) as a probable chemical mutagen. The results revealed a significantly higher level of DNA damage at all concentrations of Cypermethrin tested compared to control and background level (p < 0.05). We have standardized the technique for one of the most common native fish species that will be useful for biomonitoring genotoxicity in polluted waters of the region. SN - 1432-0800 UR - https://www.unboundmedicine.com/medline/citation/19466374/Alkaline_comet_assay_for_genotoxic_effect_detection_in_neotropical_fish_Prochilodus_lineatus__Pisces_Curimatidae__ L2 - https://dx.doi.org/10.1007/s00128-009-9771-z DB - PRIME DP - Unbound Medicine ER -