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In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb.
J Ethnopharmacol. 2009 Jul 30; 124(3):646-8.JE

Abstract

AIM OF THE STUDY

To assay the in vitro xanthine oxidase inhibitory activity of the various fractions of the hydromethanolic extract of the leaves of Erythrina stricta and to determine its enzyme inhibition mechanism.

MATERIALS AND METHODS

Xanthine oxidase inhibitory activity was assayed spectrophotometrically under aerobic conditions and the degree of enzyme inhibition was determined by measuring the increase in absorbance at 295 nm associated with uric acid formation. Enzyme kinetics was carried out using Lineweaver-Burk plots using xanthine as the substrate.

RESULTS

Among the fractions tested, the chloroform fraction exhibited highest potency (IC(50) 21.2+/-1.6 microg/ml) followed by the pet-ether (IC(50) 30.2+/-2.2 microg/ml), ethyl acetate (IC(50) 44.9+/-1.4 microg/ml) and residual (IC(50) 100+/-3.3 microg/ml) fractions. The IC(50) value of allopurinol used, as the standard was 6.1+/-0.3 microg/ml. Enzyme inhibition mechanism indicated that the mode of inhibition was of a mixed type.

CONCLUSION

These results suggest that the use of Erythrina stricta for the treatment of gout could be attributed to its xanthine oxidase inhibitory activity.

Authors+Show Affiliations

Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641044, India. umasaravanaprakash@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19467311

Citation

Umamaheswari, M, et al. "In Vitro Xanthine Oxidase Inhibitory Activity of the Fractions of Erythrina Stricta Roxb." Journal of Ethnopharmacology, vol. 124, no. 3, 2009, pp. 646-8.
Umamaheswari M, Asokkumar K, Sivashanmugam AT, et al. In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb. J Ethnopharmacol. 2009;124(3):646-8.
Umamaheswari, M., Asokkumar, K., Sivashanmugam, A. T., Remyaraju, A., Subhadradevi, V., & Ravi, T. K. (2009). In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb. Journal of Ethnopharmacology, 124(3), 646-8. https://doi.org/10.1016/j.jep.2009.05.018
Umamaheswari M, et al. In Vitro Xanthine Oxidase Inhibitory Activity of the Fractions of Erythrina Stricta Roxb. J Ethnopharmacol. 2009 Jul 30;124(3):646-8. PubMed PMID: 19467311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb. AU - Umamaheswari,M, AU - Asokkumar,K, AU - Sivashanmugam,A T, AU - Remyaraju,A, AU - Subhadradevi,V, AU - Ravi,T K, Y1 - 2009/05/23/ PY - 2008/04/02/received PY - 2009/04/16/revised PY - 2009/05/15/accepted PY - 2009/5/27/entrez PY - 2009/5/27/pubmed PY - 2009/10/15/medline SP - 646 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 124 IS - 3 N2 - AIM OF THE STUDY: To assay the in vitro xanthine oxidase inhibitory activity of the various fractions of the hydromethanolic extract of the leaves of Erythrina stricta and to determine its enzyme inhibition mechanism. MATERIALS AND METHODS: Xanthine oxidase inhibitory activity was assayed spectrophotometrically under aerobic conditions and the degree of enzyme inhibition was determined by measuring the increase in absorbance at 295 nm associated with uric acid formation. Enzyme kinetics was carried out using Lineweaver-Burk plots using xanthine as the substrate. RESULTS: Among the fractions tested, the chloroform fraction exhibited highest potency (IC(50) 21.2+/-1.6 microg/ml) followed by the pet-ether (IC(50) 30.2+/-2.2 microg/ml), ethyl acetate (IC(50) 44.9+/-1.4 microg/ml) and residual (IC(50) 100+/-3.3 microg/ml) fractions. The IC(50) value of allopurinol used, as the standard was 6.1+/-0.3 microg/ml. Enzyme inhibition mechanism indicated that the mode of inhibition was of a mixed type. CONCLUSION: These results suggest that the use of Erythrina stricta for the treatment of gout could be attributed to its xanthine oxidase inhibitory activity. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19467311/In_vitro_xanthine_oxidase_inhibitory_activity_of_the_fractions_of_Erythrina_stricta_Roxb_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00302-X DB - PRIME DP - Unbound Medicine ER -