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The large Bacillus plasmid pTB19 contains two integrated rolling-circle plasmids carrying mobilization functions.
Plasmid 1991; 26(1):30-9P

Abstract

Plasmid pTB19 is a 27-kb plasmid originating from a thermophilic Bacillus species. It was shown previously that pTB19 contains an integrated copy of the rolling-circle type plasmid pTB913. Here we describe the analysis of a 4324-bp region of pTB19 conferring resistance to tetracycline. The nucleotide sequence of this region revealed all the characteristics of a second plasmid replicating via the rolling-circle mechanism. This sequence contained (i) the tetracycline resistance marker of pTB19, which is highly similar to other tetL-genes of gram-positive bacteria; (ii) a hybrid mob gene, which bears relatedness to both the mob-genes of pUB110 and pTB913; (iii) a palU type minus origin identical to those of pUB110 and pTB913; and (iv) a plus origin of replication similar to that of pTB913. A repB-type replication initiation gene sequence identical to that of pTB913 was present, which lacked the middle part (492 bp), thus preventing autonomous replication of this region. The hybrid mob gene was functional in conjugative mobilization of plasmids between strains of Bacillus subtilis.

Authors+Show Affiliations

Department of Genetics, University of Groningen, Haren, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1946749

Citation

Oskam, L, et al. "The Large Bacillus Plasmid pTB19 Contains Two Integrated Rolling-circle Plasmids Carrying Mobilization Functions." Plasmid, vol. 26, no. 1, 1991, pp. 30-9.
Oskam L, Hillenga DJ, Venema G, et al. The large Bacillus plasmid pTB19 contains two integrated rolling-circle plasmids carrying mobilization functions. Plasmid. 1991;26(1):30-9.
Oskam, L., Hillenga, D. J., Venema, G., & Bron, S. (1991). The large Bacillus plasmid pTB19 contains two integrated rolling-circle plasmids carrying mobilization functions. Plasmid, 26(1), pp. 30-9.
Oskam L, et al. The Large Bacillus Plasmid pTB19 Contains Two Integrated Rolling-circle Plasmids Carrying Mobilization Functions. Plasmid. 1991;26(1):30-9. PubMed PMID: 1946749.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The large Bacillus plasmid pTB19 contains two integrated rolling-circle plasmids carrying mobilization functions. AU - Oskam,L, AU - Hillenga,D J, AU - Venema,G, AU - Bron,S, PY - 1991/7/1/pubmed PY - 1991/7/1/medline PY - 1991/7/1/entrez SP - 30 EP - 9 JF - Plasmid JO - Plasmid VL - 26 IS - 1 N2 - Plasmid pTB19 is a 27-kb plasmid originating from a thermophilic Bacillus species. It was shown previously that pTB19 contains an integrated copy of the rolling-circle type plasmid pTB913. Here we describe the analysis of a 4324-bp region of pTB19 conferring resistance to tetracycline. The nucleotide sequence of this region revealed all the characteristics of a second plasmid replicating via the rolling-circle mechanism. This sequence contained (i) the tetracycline resistance marker of pTB19, which is highly similar to other tetL-genes of gram-positive bacteria; (ii) a hybrid mob gene, which bears relatedness to both the mob-genes of pUB110 and pTB913; (iii) a palU type minus origin identical to those of pUB110 and pTB913; and (iv) a plus origin of replication similar to that of pTB913. A repB-type replication initiation gene sequence identical to that of pTB913 was present, which lacked the middle part (492 bp), thus preventing autonomous replication of this region. The hybrid mob gene was functional in conjugative mobilization of plasmids between strains of Bacillus subtilis. SN - 0147-619X UR - https://www.unboundmedicine.com/medline/citation/1946749/The_large_Bacillus_plasmid_pTB19_contains_two_integrated_rolling_circle_plasmids_carrying_mobilization_functions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0147-619X(91)90034-T DB - PRIME DP - Unbound Medicine ER -