A novel water-soluble beta-(1-->6)-D-glucan isolated from the fruit bodies of Bulgaria inquinans (Fries).Carbohydr Res. 2009 Jul 06; 344(10):1254-8.CR
A low molecular-weight polysaccharide named BIWP2 was purified from the fruit bodies of Bulgaria Inquinans (Fries) via hot-water extraction, followed by freeze-thawing and gel filtration chromatography on Sephadex G-75. Monosaccharide composition analysis revealed that BIWP2 contained exclusively glucose. High performance size exclusion chromatography (HPSEC) showed that it was a homogeneous polysaccharide fraction. Its molecular weight was estimated to be 2.6 KD and the polydispersity index (M(w)/M(n)) was calculated to be 1.4. Periodate oxidation, methylation, and NMR analyses indicated that BIWP2 was a linear beta-(1-->6)-D-glucan without side chains. This is the first time to report a linear beta-(1-->6)-D-glucan with low molecular weight in non-lichenized ascomycete.