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Anti-apoptotic and pro-apoptotic gene expression evaluated from eutopic endometrium in the proliferative phase of the menstrual cycle among women with endometriosis and healthy controls.
Eur J Obstet Gynecol Reprod Biol. 2009 Aug; 145(2):172-6.EJ

Abstract

OBJECTIVE

Endometriosis affects 5-15% of women in the general population and 40% of women seeking infertility evaluation. Its etiology and pathogenesis is controversial. Abnormalities of genes involved in the regulation of apoptosis have been thought to play a role in origin. Hence, we investigated the expression of pro-apoptotic and anti-apoptotic genes in eutopic endometrium from women with endometriosis and healthy controls in relation to disease occurrence and severity.

STUDY DESIGN

A prospective study in women undergoing laparoscopic surgery for pelvic pain was conducted. In total, 45 women (30 healthy controls and 15 patients) matched inclusion criteria. The mRNA expression of apoptotic genes (p53, Bcl-x(L,S) and Bax) from eutopic endometrium was detected by RT-PCR.

RESULTS

A significant increase in expression of mRNA p53 (1.42 versus 1.02; p<0.05), and Bcl-x(S) (0.41 versus 0.19; p=0.0006) was found in women with endometriosis compared to healthy controls. Insignificantly increased expression was found for Bax (1.22 versus 1.15). The expression of anti-apoptotic Bcl-x(L) was unchanged (1.08 versus 1.07). The Bcl-x(L)/Bcl-x(S) ratio was twofold higher (5.63 versus 2.63) in controls. By stratifying patients by disease stage we have revealed an increased mRNA expression of apoptotic genes in patients with grades III-IV endometriosis compared to those with grades I-II. However, the difference was significant only for Bcl-x(S) expression (p<0.05).

CONCLUSIONS

Results suggest that an increased transcription of pro-apoptotic genes (p53 and Bcl-x(S)) in eutopic endometrium is significantly associated with endometriosis, which indicates dysregulation of apoptotic gene transcription associated with disease.

Authors+Show Affiliations

Clinic of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic. zubor@jfmed.uniba.skNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19467764

Citation

Zubor, Pavol, et al. "Anti-apoptotic and Pro-apoptotic Gene Expression Evaluated From Eutopic Endometrium in the Proliferative Phase of the Menstrual Cycle Among Women With Endometriosis and Healthy Controls." European Journal of Obstetrics, Gynecology, and Reproductive Biology, vol. 145, no. 2, 2009, pp. 172-6.
Zubor P, Hatok J, Galo S, et al. Anti-apoptotic and pro-apoptotic gene expression evaluated from eutopic endometrium in the proliferative phase of the menstrual cycle among women with endometriosis and healthy controls. Eur J Obstet Gynecol Reprod Biol. 2009;145(2):172-6.
Zubor, P., Hatok, J., Galo, S., Dokus, K., Klobusiakova, D., Danko, J., & Racay, P. (2009). Anti-apoptotic and pro-apoptotic gene expression evaluated from eutopic endometrium in the proliferative phase of the menstrual cycle among women with endometriosis and healthy controls. European Journal of Obstetrics, Gynecology, and Reproductive Biology, 145(2), 172-6. https://doi.org/10.1016/j.ejogrb.2009.04.024
Zubor P, et al. Anti-apoptotic and Pro-apoptotic Gene Expression Evaluated From Eutopic Endometrium in the Proliferative Phase of the Menstrual Cycle Among Women With Endometriosis and Healthy Controls. Eur J Obstet Gynecol Reprod Biol. 2009;145(2):172-6. PubMed PMID: 19467764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-apoptotic and pro-apoptotic gene expression evaluated from eutopic endometrium in the proliferative phase of the menstrual cycle among women with endometriosis and healthy controls. AU - Zubor,Pavol, AU - Hatok,Jozef, AU - Galo,Silvester, AU - Dokus,Karol, AU - Klobusiakova,Dasa, AU - Danko,Jan, AU - Racay,Peter, Y1 - 2009/05/20/ PY - 2008/11/19/received PY - 2009/03/19/revised PY - 2009/04/21/accepted PY - 2009/5/27/entrez PY - 2009/5/27/pubmed PY - 2009/10/23/medline SP - 172 EP - 6 JF - European journal of obstetrics, gynecology, and reproductive biology JO - Eur. J. Obstet. Gynecol. Reprod. Biol. VL - 145 IS - 2 N2 - OBJECTIVE: Endometriosis affects 5-15% of women in the general population and 40% of women seeking infertility evaluation. Its etiology and pathogenesis is controversial. Abnormalities of genes involved in the regulation of apoptosis have been thought to play a role in origin. Hence, we investigated the expression of pro-apoptotic and anti-apoptotic genes in eutopic endometrium from women with endometriosis and healthy controls in relation to disease occurrence and severity. STUDY DESIGN: A prospective study in women undergoing laparoscopic surgery for pelvic pain was conducted. In total, 45 women (30 healthy controls and 15 patients) matched inclusion criteria. The mRNA expression of apoptotic genes (p53, Bcl-x(L,S) and Bax) from eutopic endometrium was detected by RT-PCR. RESULTS: A significant increase in expression of mRNA p53 (1.42 versus 1.02; p<0.05), and Bcl-x(S) (0.41 versus 0.19; p=0.0006) was found in women with endometriosis compared to healthy controls. Insignificantly increased expression was found for Bax (1.22 versus 1.15). The expression of anti-apoptotic Bcl-x(L) was unchanged (1.08 versus 1.07). The Bcl-x(L)/Bcl-x(S) ratio was twofold higher (5.63 versus 2.63) in controls. By stratifying patients by disease stage we have revealed an increased mRNA expression of apoptotic genes in patients with grades III-IV endometriosis compared to those with grades I-II. However, the difference was significant only for Bcl-x(S) expression (p<0.05). CONCLUSIONS: Results suggest that an increased transcription of pro-apoptotic genes (p53 and Bcl-x(S)) in eutopic endometrium is significantly associated with endometriosis, which indicates dysregulation of apoptotic gene transcription associated with disease. SN - 1872-7654 UR - https://www.unboundmedicine.com/medline/citation/19467764/Anti_apoptotic_and_pro_apoptotic_gene_expression_evaluated_from_eutopic_endometrium_in_the_proliferative_phase_of_the_menstrual_cycle_among_women_with_endometriosis_and_healthy_controls_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301-2115(09)00299-1 DB - PRIME DP - Unbound Medicine ER -