Tags

Type your tag names separated by a space and hit enter

Echinacoside elicits endothelium-dependent relaxation in rat aortic rings via an NO-cGMP pathway.
Planta Med. 2009 Oct; 75(13):1400-4.PM

Abstract

The aim of this study was to identify and elucidate the vasorelaxant activity of echinacoside, a phenylethanoid glycoside isolated from the medicinal herb Cistanche tubulosa, and its possible underlying mechanism on isolated rat thoracic aortic rings pre-contracted with phenylephrine (PE, 1 microM) and KCl (60 mM). Echinacoside (30-300 microM) exhibited an acute relaxation in endothelium-intact rings in a concentration-dependent manner, while this relaxation was significantly inhibited in endothelium-denuded condition and in the presence of the endothelial nitric oxide synthase (eNOS) inhibitor, N(W)-nitro-L-arginine methyl ester (L-NNA, 100 microM), an unselective soluble guanylate cyclase blocker, methylene blue (10 microM), the selective sGC inhibitor 1 H-[1, 2, 4]oxadiazolo[4,3- A]quinoxalin-1-one (ODQ, 1 microM); in addition, atropine (1 microM), a selective muscarinic receptor antagonist, partially affected the relaxation. However, the cyclooxygenase inhibitor indomethacin (5 microM) had no influence on the action. Echinacoside enhanced the cyclic guanosine monophosphate (cGMP) production in aortic rings contracted with PE. These results indicate for the first time that echinacoside mediates the endothelium-dependent vasodilator action in rat thoracic aortic rings through nitric oxide (NO)-cGMP pathway.

Authors+Show Affiliations

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19468974

Citation

He, Wen-Jun, et al. "Echinacoside Elicits Endothelium-dependent Relaxation in Rat Aortic Rings Via an NO-cGMP Pathway." Planta Medica, vol. 75, no. 13, 2009, pp. 1400-4.
He WJ, Fang TH, Ma X, et al. Echinacoside elicits endothelium-dependent relaxation in rat aortic rings via an NO-cGMP pathway. Planta Med. 2009;75(13):1400-4.
He, W. J., Fang, T. H., Ma, X., Zhang, K., Ma, Z. Z., & Tu, P. F. (2009). Echinacoside elicits endothelium-dependent relaxation in rat aortic rings via an NO-cGMP pathway. Planta Medica, 75(13), 1400-4. https://doi.org/10.1055/s-0029-1185745
He WJ, et al. Echinacoside Elicits Endothelium-dependent Relaxation in Rat Aortic Rings Via an NO-cGMP Pathway. Planta Med. 2009;75(13):1400-4. PubMed PMID: 19468974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Echinacoside elicits endothelium-dependent relaxation in rat aortic rings via an NO-cGMP pathway. AU - He,Wen-Jun, AU - Fang,Tai-Hui, AU - Ma,Xu, AU - Zhang,Ke, AU - Ma,Zhi-Zhong, AU - Tu,Peng-Fei, Y1 - 2009/05/25/ PY - 2009/5/27/entrez PY - 2009/5/27/pubmed PY - 2010/3/23/medline SP - 1400 EP - 4 JF - Planta medica JO - Planta Med VL - 75 IS - 13 N2 - The aim of this study was to identify and elucidate the vasorelaxant activity of echinacoside, a phenylethanoid glycoside isolated from the medicinal herb Cistanche tubulosa, and its possible underlying mechanism on isolated rat thoracic aortic rings pre-contracted with phenylephrine (PE, 1 microM) and KCl (60 mM). Echinacoside (30-300 microM) exhibited an acute relaxation in endothelium-intact rings in a concentration-dependent manner, while this relaxation was significantly inhibited in endothelium-denuded condition and in the presence of the endothelial nitric oxide synthase (eNOS) inhibitor, N(W)-nitro-L-arginine methyl ester (L-NNA, 100 microM), an unselective soluble guanylate cyclase blocker, methylene blue (10 microM), the selective sGC inhibitor 1 H-[1, 2, 4]oxadiazolo[4,3- A]quinoxalin-1-one (ODQ, 1 microM); in addition, atropine (1 microM), a selective muscarinic receptor antagonist, partially affected the relaxation. However, the cyclooxygenase inhibitor indomethacin (5 microM) had no influence on the action. Echinacoside enhanced the cyclic guanosine monophosphate (cGMP) production in aortic rings contracted with PE. These results indicate for the first time that echinacoside mediates the endothelium-dependent vasodilator action in rat thoracic aortic rings through nitric oxide (NO)-cGMP pathway. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/19468974/Echinacoside_elicits_endothelium_dependent_relaxation_in_rat_aortic_rings_via_an_NO_cGMP_pathway_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1185745 DB - PRIME DP - Unbound Medicine ER -