Single-step purification of native miraculin using immobilized metal-affinity chromatography.J Agric Food Chem. 2009 Jun 24; 57(12):5148-51.JA
Miraculin is a taste-modifying protein that can be isolated from miracle fruit (Richadella dulcifica), a shrub native to West Africa. It is able to turn a sour taste into a sweet taste. The commercial exploitation of this sweetness-modifying protein is underway, and a fast and efficient purification method to extract the protein is needed. We succeeded in purifying miraculin from miracle fruit in a single-step purification using immobilized metal-affinity chromatography (IMAC). The purified miraculin exhibited high purity (>95%) in reverse-phase high-performance liquid chromatography. We also demonstrated the necessity of its structure for binding to the nickel-IMAC column.