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Single-step purification of native miraculin using immobilized metal-affinity chromatography.
J Agric Food Chem. 2009 Jun 24; 57(12):5148-51.JA

Abstract

Miraculin is a taste-modifying protein that can be isolated from miracle fruit (Richadella dulcifica), a shrub native to West Africa. It is able to turn a sour taste into a sweet taste. The commercial exploitation of this sweetness-modifying protein is underway, and a fast and efficient purification method to extract the protein is needed. We succeeded in purifying miraculin from miracle fruit in a single-step purification using immobilized metal-affinity chromatography (IMAC). The purified miraculin exhibited high purity (>95%) in reverse-phase high-performance liquid chromatography. We also demonstrated the necessity of its structure for binding to the nickel-IMAC column.

Authors+Show Affiliations

Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19469504

Citation

Duhita, Narendra, et al. "Single-step Purification of Native Miraculin Using Immobilized Metal-affinity Chromatography." Journal of Agricultural and Food Chemistry, vol. 57, no. 12, 2009, pp. 5148-51.
Duhita N, Hiwasa-Tanase K, Yoshida S, et al. Single-step purification of native miraculin using immobilized metal-affinity chromatography. J Agric Food Chem. 2009;57(12):5148-51.
Duhita, N., Hiwasa-Tanase, K., Yoshida, S., & Ezura, H. (2009). Single-step purification of native miraculin using immobilized metal-affinity chromatography. Journal of Agricultural and Food Chemistry, 57(12), 5148-51. https://doi.org/10.1021/jf9004065
Duhita N, et al. Single-step Purification of Native Miraculin Using Immobilized Metal-affinity Chromatography. J Agric Food Chem. 2009 Jun 24;57(12):5148-51. PubMed PMID: 19469504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-step purification of native miraculin using immobilized metal-affinity chromatography. AU - Duhita,Narendra, AU - Hiwasa-Tanase,Kyoko, AU - Yoshida,Shigeki, AU - Ezura,Hiroshi, PY - 2009/5/28/entrez PY - 2009/5/28/pubmed PY - 2009/9/11/medline SP - 5148 EP - 51 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 12 N2 - Miraculin is a taste-modifying protein that can be isolated from miracle fruit (Richadella dulcifica), a shrub native to West Africa. It is able to turn a sour taste into a sweet taste. The commercial exploitation of this sweetness-modifying protein is underway, and a fast and efficient purification method to extract the protein is needed. We succeeded in purifying miraculin from miracle fruit in a single-step purification using immobilized metal-affinity chromatography (IMAC). The purified miraculin exhibited high purity (>95%) in reverse-phase high-performance liquid chromatography. We also demonstrated the necessity of its structure for binding to the nickel-IMAC column. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19469504/Single_step_purification_of_native_miraculin_using_immobilized_metal_affinity_chromatography_ L2 - https://doi.org/10.1021/jf9004065 DB - PRIME DP - Unbound Medicine ER -