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Epigenetic chromatin modifiers in barley: I. Cloning, mapping and expression analysis of the plant specific HD2 family of histone deacetylases from barley, during seed development and after hormonal treatment.
Physiol Plant. 2009 Jul; 136(3):358-68.PP

Abstract

Epigenetic phenomena have been associated with modifications of chromatin structure. These are achieved, in part, by histone post-translational modifications including acetylations and deacetylations, the later being catalyzed by histone deacetylaces (HDACs). Eukaryotic HDACs are grouped into three major families, RPD3/HDA1, SIR2 and the plant-specific HD2. HDAC genes have been analyzed from model plants such as Arabidopsis, rice and maize and have been shown to be involved in various cellular processes including seed development, vegetative and reproductive growth and responses to abiotic and biotic stress, but reports on HDACs from other crops are limited. In this work two full-length cDNAs (HvHDAC2-1 and HvHDAC2-2) encoding two members of the plant-specific HD2 family, respectively, were isolated and characterized from barley (Hordeum vulgare), an agronomically important cereal crop. HvHDAC2-1 and HvHDAC2-2 were mapped on barley chromosomes 1H and 3H, respectively, which could prove useful in developing markers for marker-assisted selection in breeding programs. Expression analysis of the barley HD2 genes demonstrated that they are expressed in all tissues and seed developmental stages examined. Significant differences were observed among tissues and seed stages, and between cultivars with varying seed size, suggesting an association of these genes with seed development. Furthermore, the HD2 genes from barley were found to respond to treatments with plant stress-related hormones such as jasmonic acid (JA), abscisic acid (ABA) and salicylic acid (SA) implying an association of these genes with plant resistance to biotic and abiotic stress. The expression pattern of HD2 genes suggests a possible role for these genes in the epigenetic regulation of seed development and stress response.

Authors+Show Affiliations

Centre for Research & Technology, Institute of Agrobiotechnology, Thermi-Thessaloniki, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19470089

Citation

Demetriou, Kyproula, et al. "Epigenetic Chromatin Modifiers in Barley: I. Cloning, Mapping and Expression Analysis of the Plant Specific HD2 Family of Histone Deacetylases From Barley, During Seed Development and After Hormonal Treatment." Physiologia Plantarum, vol. 136, no. 3, 2009, pp. 358-68.
Demetriou K, Kapazoglou A, Tondelli A, et al. Epigenetic chromatin modifiers in barley: I. Cloning, mapping and expression analysis of the plant specific HD2 family of histone deacetylases from barley, during seed development and after hormonal treatment. Physiol Plant. 2009;136(3):358-68.
Demetriou, K., Kapazoglou, A., Tondelli, A., Francia, E., Stanca, M. A., Bladenopoulos, K., & Tsaftaris, A. S. (2009). Epigenetic chromatin modifiers in barley: I. Cloning, mapping and expression analysis of the plant specific HD2 family of histone deacetylases from barley, during seed development and after hormonal treatment. Physiologia Plantarum, 136(3), 358-68. https://doi.org/10.1111/j.1399-3054.2009.01236.x
Demetriou K, et al. Epigenetic Chromatin Modifiers in Barley: I. Cloning, Mapping and Expression Analysis of the Plant Specific HD2 Family of Histone Deacetylases From Barley, During Seed Development and After Hormonal Treatment. Physiol Plant. 2009;136(3):358-68. PubMed PMID: 19470089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epigenetic chromatin modifiers in barley: I. Cloning, mapping and expression analysis of the plant specific HD2 family of histone deacetylases from barley, during seed development and after hormonal treatment. AU - Demetriou,Kyproula, AU - Kapazoglou,Aliki, AU - Tondelli,Alessandro, AU - Francia,Enrico, AU - Stanca,Michele A, AU - Bladenopoulos,Konstantinos, AU - Tsaftaris,Athanasios S, Y1 - 2009/04/04/ PY - 2009/5/28/entrez PY - 2009/5/28/pubmed PY - 2009/7/21/medline SP - 358 EP - 68 JF - Physiologia plantarum JO - Physiol Plant VL - 136 IS - 3 N2 - Epigenetic phenomena have been associated with modifications of chromatin structure. These are achieved, in part, by histone post-translational modifications including acetylations and deacetylations, the later being catalyzed by histone deacetylaces (HDACs). Eukaryotic HDACs are grouped into three major families, RPD3/HDA1, SIR2 and the plant-specific HD2. HDAC genes have been analyzed from model plants such as Arabidopsis, rice and maize and have been shown to be involved in various cellular processes including seed development, vegetative and reproductive growth and responses to abiotic and biotic stress, but reports on HDACs from other crops are limited. In this work two full-length cDNAs (HvHDAC2-1 and HvHDAC2-2) encoding two members of the plant-specific HD2 family, respectively, were isolated and characterized from barley (Hordeum vulgare), an agronomically important cereal crop. HvHDAC2-1 and HvHDAC2-2 were mapped on barley chromosomes 1H and 3H, respectively, which could prove useful in developing markers for marker-assisted selection in breeding programs. Expression analysis of the barley HD2 genes demonstrated that they are expressed in all tissues and seed developmental stages examined. Significant differences were observed among tissues and seed stages, and between cultivars with varying seed size, suggesting an association of these genes with seed development. Furthermore, the HD2 genes from barley were found to respond to treatments with plant stress-related hormones such as jasmonic acid (JA), abscisic acid (ABA) and salicylic acid (SA) implying an association of these genes with plant resistance to biotic and abiotic stress. The expression pattern of HD2 genes suggests a possible role for these genes in the epigenetic regulation of seed development and stress response. SN - 1399-3054 UR - https://www.unboundmedicine.com/medline/citation/19470089/Epigenetic_chromatin_modifiers_in_barley:_I__Cloning_mapping_and_expression_analysis_of_the_plant_specific_HD2_family_of_histone_deacetylases_from_barley_during_seed_development_and_after_hormonal_treatment_ L2 - https://doi.org/10.1111/j.1399-3054.2009.01236.x DB - PRIME DP - Unbound Medicine ER -