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Large-scale mRNA analysis of female skeletal muscles during 60 days of bed rest with and without exercise or dietary protein supplementation as countermeasures.
Physiol Genomics. 2009 Aug 07; 38(3):291-302.PG

Abstract

Microgravity has a dramatic impact on human physiology, illustrated in particular, with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for defining efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on the transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group, n = 8), and the potential beneficial impact of protein supplementation (BRN group, n = 8) and of a combined resistance and aerobic training (BRE group, n = 8). Gene expression profiles were obtained using a customized microarray containing 6,681 muscles-relevant genes. A two-class statistical analysis was applied on 2,103 genes with consolidated expression in BRC, BRN, and BRE groups. We identified 472 and 207 mRNAs whose expression was modified in SOL and VL from BRC group, respectively. Further clustering analysis, identifying relevant biological mechanisms and pathways, reported five main subclusters. Three are composed of upregulated mRNAs involved mainly in nucleic acid and protein metabolism, and two made up of downregulated transcripts encoding components involved in energy metabolism. Exercise countermeasure demonstrated drastic compensatory effects, decreasing the number of differentially expressed mRNAs by 89 and 96% in SOL and VL, respectively. In contrast, nutrition countermeasure had moderate effects and decreased the number of differentially-expressed transcripts by 40 and 25% in SOL and VL. Together, these data present a systematic, global and comprehensive view of the adaptive response of female muscle to long-term atrophy.

Authors+Show Affiliations

Department of Cellular and Molecular Medicine, Centre for Neuromuscular Disease, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19470803

Citation

Chopard, A, et al. "Large-scale mRNA Analysis of Female Skeletal Muscles During 60 Days of Bed Rest With and Without Exercise or Dietary Protein Supplementation as Countermeasures." Physiological Genomics, vol. 38, no. 3, 2009, pp. 291-302.
Chopard A, Lecunff M, Danger R, et al. Large-scale mRNA analysis of female skeletal muscles during 60 days of bed rest with and without exercise or dietary protein supplementation as countermeasures. Physiol Genomics. 2009;38(3):291-302.
Chopard, A., Lecunff, M., Danger, R., Lamirault, G., Bihouee, A., Teusan, R., Jasmin, B. J., Marini, J. F., & Leger, J. J. (2009). Large-scale mRNA analysis of female skeletal muscles during 60 days of bed rest with and without exercise or dietary protein supplementation as countermeasures. Physiological Genomics, 38(3), 291-302. https://doi.org/10.1152/physiolgenomics.00036.2009
Chopard A, et al. Large-scale mRNA Analysis of Female Skeletal Muscles During 60 Days of Bed Rest With and Without Exercise or Dietary Protein Supplementation as Countermeasures. Physiol Genomics. 2009 Aug 7;38(3):291-302. PubMed PMID: 19470803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large-scale mRNA analysis of female skeletal muscles during 60 days of bed rest with and without exercise or dietary protein supplementation as countermeasures. AU - Chopard,A, AU - Lecunff,M, AU - Danger,R, AU - Lamirault,G, AU - Bihouee,A, AU - Teusan,R, AU - Jasmin,B J, AU - Marini,J F, AU - Leger,J J, Y1 - 2009/05/26/ PY - 2009/5/28/entrez PY - 2009/5/28/pubmed PY - 2010/1/14/medline SP - 291 EP - 302 JF - Physiological genomics JO - Physiol Genomics VL - 38 IS - 3 N2 - Microgravity has a dramatic impact on human physiology, illustrated in particular, with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for defining efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on the transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group, n = 8), and the potential beneficial impact of protein supplementation (BRN group, n = 8) and of a combined resistance and aerobic training (BRE group, n = 8). Gene expression profiles were obtained using a customized microarray containing 6,681 muscles-relevant genes. A two-class statistical analysis was applied on 2,103 genes with consolidated expression in BRC, BRN, and BRE groups. We identified 472 and 207 mRNAs whose expression was modified in SOL and VL from BRC group, respectively. Further clustering analysis, identifying relevant biological mechanisms and pathways, reported five main subclusters. Three are composed of upregulated mRNAs involved mainly in nucleic acid and protein metabolism, and two made up of downregulated transcripts encoding components involved in energy metabolism. Exercise countermeasure demonstrated drastic compensatory effects, decreasing the number of differentially expressed mRNAs by 89 and 96% in SOL and VL, respectively. In contrast, nutrition countermeasure had moderate effects and decreased the number of differentially-expressed transcripts by 40 and 25% in SOL and VL. Together, these data present a systematic, global and comprehensive view of the adaptive response of female muscle to long-term atrophy. SN - 1531-2267 UR - https://www.unboundmedicine.com/medline/citation/19470803/Large_scale_mRNA_analysis_of_female_skeletal_muscles_during_60_days_of_bed_rest_with_and_without_exercise_or_dietary_protein_supplementation_as_countermeasures_ L2 - https://journals.physiology.org/doi/10.1152/physiolgenomics.00036.2009?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -