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Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat.
Ecotoxicol Environ Saf. 2009 Sep; 72(6):1687-93.EE

Abstract

Prometryne is one of the herbicides widely used for controlling weed/grass in agricultural practice. However, whether it has an adverse effect on crops is unknown. In this study, we investigated prometryne-induced oxidative stress in wheat (Triticum aestivum). Wheat plants were grown in soils with prometryne at 0-24 mgkg(-1) soil. The growth of wheat treated with prometryne was inhibited. Chlorophyll content significantly decreased even at the low level of prometryne (4 mgkg(-1) soil). Accumulation of thiobarbituric acid reactive substances (TBARS), an indicator of cellular peroxidation, increased, suggesting oxidative damage to the plants. The prometryne-induced oxidative stress triggered significant changes in activities of a variety of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and glutathione S-transferase (GST). Activities of the enzymes showed a general increase at low prometryne concentrations but a decrease at high levels. Analysis of non-denaturing polyacrylamide gel electrophoresis (PAGE) confirmed the results. To get an insight into the molecular response, a qRT-PCR-based assay was performed to analyze the transcript abundance of Cu/Zn-SOD and GST with prometryne exposure. Our analysis revealed that both genes displayed up-regulated expression patterns similar to the activities of the two enzymes. These data imply that prometryne-induced oxidative stress was responsible for the disturbance of the growth and antioxidant defensive systems in wheat plants.

Authors+Show Affiliations

Department of Applied Chemistry, College of Sciences, Nanjing Agricultural University, Weigang No. 1, Outside the Zhongshan Men, Building of Chemistry, Nanjing 210095, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19473703

Citation

Jiang, Lei, and Hong Yang. "Prometryne-induced Oxidative Stress and Impact On Antioxidant Enzymes in Wheat." Ecotoxicology and Environmental Safety, vol. 72, no. 6, 2009, pp. 1687-93.
Jiang L, Yang H. Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat. Ecotoxicol Environ Saf. 2009;72(6):1687-93.
Jiang, L., & Yang, H. (2009). Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat. Ecotoxicology and Environmental Safety, 72(6), 1687-93. https://doi.org/10.1016/j.ecoenv.2009.04.025
Jiang L, Yang H. Prometryne-induced Oxidative Stress and Impact On Antioxidant Enzymes in Wheat. Ecotoxicol Environ Saf. 2009;72(6):1687-93. PubMed PMID: 19473703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat. AU - Jiang,Lei, AU - Yang,Hong, Y1 - 2009/05/27/ PY - 2008/08/12/received PY - 2009/04/14/revised PY - 2009/04/18/accepted PY - 2009/5/29/entrez PY - 2009/5/29/pubmed PY - 2009/10/1/medline SP - 1687 EP - 93 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 72 IS - 6 N2 - Prometryne is one of the herbicides widely used for controlling weed/grass in agricultural practice. However, whether it has an adverse effect on crops is unknown. In this study, we investigated prometryne-induced oxidative stress in wheat (Triticum aestivum). Wheat plants were grown in soils with prometryne at 0-24 mgkg(-1) soil. The growth of wheat treated with prometryne was inhibited. Chlorophyll content significantly decreased even at the low level of prometryne (4 mgkg(-1) soil). Accumulation of thiobarbituric acid reactive substances (TBARS), an indicator of cellular peroxidation, increased, suggesting oxidative damage to the plants. The prometryne-induced oxidative stress triggered significant changes in activities of a variety of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and glutathione S-transferase (GST). Activities of the enzymes showed a general increase at low prometryne concentrations but a decrease at high levels. Analysis of non-denaturing polyacrylamide gel electrophoresis (PAGE) confirmed the results. To get an insight into the molecular response, a qRT-PCR-based assay was performed to analyze the transcript abundance of Cu/Zn-SOD and GST with prometryne exposure. Our analysis revealed that both genes displayed up-regulated expression patterns similar to the activities of the two enzymes. These data imply that prometryne-induced oxidative stress was responsible for the disturbance of the growth and antioxidant defensive systems in wheat plants. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/19473703/Prometryne_induced_oxidative_stress_and_impact_on_antioxidant_enzymes_in_wheat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(09)00094-3 DB - PRIME DP - Unbound Medicine ER -