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Clinical severity and molecular typing of human rhinovirus C strains during a fall outbreak affecting hospitalized patients.
J Clin Virol. 2009 Aug; 45(4):311-7.JC

Abstract

BACKGROUND

The circulation rate and the clinical severity of infections caused by members of the new human rhinovirus C (HRV-C) species remain to be defined.

OBJECTIVES

To investigate the epidemiologic and clinical impact of HRV-C strains in a fall outbreak interesting hospitalized patients.

STUDY DESIGN

HRV species (A-C) were determined by phylogenetic analysis following amplification of two genome regions (5'NCR and VP4/VP2) by RT-PCR. HRV species were correlated with age, respiratory tract involvement, clinical symptoms, and HRV load in respiratory secretions.

RESULTS

During the first week of the period October-November 2008, single HRV infections were associated with 95% of all respiratory syndromes affecting hospitalized patients. Then, HRV infections (single+coinfections) interested about 90% of positive samples until the end of October, when they declined in frequency until reaching about 30% at the end of November. Overall, 104 HRV strains were detected and, of these, 90 could be classified by phylogenetic analysis, as follows: 45 HRV-A, 12 HRV-B, 28 HRV-C, and 5 human enterovirus D strains. HRV-C identity was confirmed by detection of cis-acting replication elements (cre) in 23/23 strains. As for severity of respiratory syndromes, unlike HRV-A and HRV-B strains, HRV-C strains were responsible for a significantly higher rate (p<0.05) of lower respiratory tract infections in the pediatric as compared to adult patient population.

CONCLUSIONS

HRV-C strains have been shown to circulate at a rate intermediate between HRV-A and HRV-B strains, showing a greater degree of clinical severity in the pediatric population.

Authors+Show Affiliations

Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19473873

Citation

Piralla, A, et al. "Clinical Severity and Molecular Typing of Human Rhinovirus C Strains During a Fall Outbreak Affecting Hospitalized Patients." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 45, no. 4, 2009, pp. 311-7.
Piralla A, Rovida F, Campanini G, et al. Clinical severity and molecular typing of human rhinovirus C strains during a fall outbreak affecting hospitalized patients. J Clin Virol. 2009;45(4):311-7.
Piralla, A., Rovida, F., Campanini, G., Rognoni, V., Marchi, A., Locatelli, F., & Gerna, G. (2009). Clinical severity and molecular typing of human rhinovirus C strains during a fall outbreak affecting hospitalized patients. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 45(4), 311-7. https://doi.org/10.1016/j.jcv.2009.04.016
Piralla A, et al. Clinical Severity and Molecular Typing of Human Rhinovirus C Strains During a Fall Outbreak Affecting Hospitalized Patients. J Clin Virol. 2009;45(4):311-7. PubMed PMID: 19473873.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical severity and molecular typing of human rhinovirus C strains during a fall outbreak affecting hospitalized patients. AU - Piralla,A, AU - Rovida,F, AU - Campanini,G, AU - Rognoni,V, AU - Marchi,A, AU - Locatelli,F, AU - Gerna,G, Y1 - 2009/05/26/ PY - 2009/04/23/received PY - 2009/04/24/accepted PY - 2009/5/29/entrez PY - 2009/5/29/pubmed PY - 2009/9/3/medline SP - 311 EP - 7 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 45 IS - 4 N2 - BACKGROUND: The circulation rate and the clinical severity of infections caused by members of the new human rhinovirus C (HRV-C) species remain to be defined. OBJECTIVES: To investigate the epidemiologic and clinical impact of HRV-C strains in a fall outbreak interesting hospitalized patients. STUDY DESIGN: HRV species (A-C) were determined by phylogenetic analysis following amplification of two genome regions (5'NCR and VP4/VP2) by RT-PCR. HRV species were correlated with age, respiratory tract involvement, clinical symptoms, and HRV load in respiratory secretions. RESULTS: During the first week of the period October-November 2008, single HRV infections were associated with 95% of all respiratory syndromes affecting hospitalized patients. Then, HRV infections (single+coinfections) interested about 90% of positive samples until the end of October, when they declined in frequency until reaching about 30% at the end of November. Overall, 104 HRV strains were detected and, of these, 90 could be classified by phylogenetic analysis, as follows: 45 HRV-A, 12 HRV-B, 28 HRV-C, and 5 human enterovirus D strains. HRV-C identity was confirmed by detection of cis-acting replication elements (cre) in 23/23 strains. As for severity of respiratory syndromes, unlike HRV-A and HRV-B strains, HRV-C strains were responsible for a significantly higher rate (p<0.05) of lower respiratory tract infections in the pediatric as compared to adult patient population. CONCLUSIONS: HRV-C strains have been shown to circulate at a rate intermediate between HRV-A and HRV-B strains, showing a greater degree of clinical severity in the pediatric population. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/19473873/Clinical_severity_and_molecular_typing_of_human_rhinovirus_C_strains_during_a_fall_outbreak_affecting_hospitalized_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(09)00179-6 DB - PRIME DP - Unbound Medicine ER -