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A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy.
J Gen Virol. 2009 Sep; 90(Pt 9):2306-16.JG

Abstract

Emiliania huxleyi virus 86 (EhV-86) belongs to the family Phycodnaviridae, a group of viruses that infect a wide range of freshwater and marine eukaryotic algae. Phycodnaviridae is one of the five families that belong to a large and phylogenetically diverse group of viruses known as nucleocytoplasmic large dsDNA viruses (NCLDVs). To date, our understanding of algal NCLDV entry is based on the entry mechanisms of members of the genera Chlorovirus and Phaeovirus, both of which consist of non-enveloped viruses that 'inject' their genome into their host via a viral inner-membrane host plasma membrane fusion mechanism, leaving an extracellular viral capsid. Using a combination of confocal and electron microscopy, this study demonstrated for the first time that EhV-86 differs from its algal virus counterparts in two fundamental areas. Firstly, its capsid is enveloped by a lipid membrane, and secondly, EhV-86 enters its host via either an endocytotic or an envelope fusion mechanism in which an intact nucleoprotein core still encapsulated by its capsid is seen in the host cytoplasm. Real-time fluorescence microscopy showed that viral internalization and virion breakdown took place within the host on a timescale of seconds. At around 4.5 h post-infection, virus progeny were released via a budding mechanism during which EhV-86 virions became enveloped with host plasma membrane. EhV-86 therefore appears to have an infection mechanism different from that employed by other algal NCLDVs, with entry and exit strategies showing a greater analogy to animal-like NCLDVs.

Authors+Show Affiliations

Marine Biological Association of the UK, Citadel Hill, Plymouth PL1 2DP, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19474246

Citation

Mackinder, Luke C M., et al. "A Unicellular Algal Virus, Emiliania Huxleyi Virus 86, Exploits an Animal-like Infection Strategy." The Journal of General Virology, vol. 90, no. Pt 9, 2009, pp. 2306-16.
Mackinder LC, Worthy CA, Biggi G, et al. A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy. J Gen Virol. 2009;90(Pt 9):2306-16.
Mackinder, L. C., Worthy, C. A., Biggi, G., Hall, M., Ryan, K. P., Varsani, A., Harper, G. M., Wilson, W. H., Brownlee, C., & Schroeder, D. C. (2009). A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy. The Journal of General Virology, 90(Pt 9), 2306-16. https://doi.org/10.1099/vir.0.011635-0
Mackinder LC, et al. A Unicellular Algal Virus, Emiliania Huxleyi Virus 86, Exploits an Animal-like Infection Strategy. J Gen Virol. 2009;90(Pt 9):2306-16. PubMed PMID: 19474246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy. AU - Mackinder,Luke C M, AU - Worthy,Charlotte A, AU - Biggi,Gaia, AU - Hall,Matthew, AU - Ryan,Keith P, AU - Varsani,Arvind, AU - Harper,Glenn M, AU - Wilson,William H, AU - Brownlee,Colin, AU - Schroeder,Declan C, Y1 - 2009/05/27/ PY - 2009/5/29/entrez PY - 2009/5/29/pubmed PY - 2009/9/25/medline SP - 2306 EP - 16 JF - The Journal of general virology JO - J Gen Virol VL - 90 IS - Pt 9 N2 - Emiliania huxleyi virus 86 (EhV-86) belongs to the family Phycodnaviridae, a group of viruses that infect a wide range of freshwater and marine eukaryotic algae. Phycodnaviridae is one of the five families that belong to a large and phylogenetically diverse group of viruses known as nucleocytoplasmic large dsDNA viruses (NCLDVs). To date, our understanding of algal NCLDV entry is based on the entry mechanisms of members of the genera Chlorovirus and Phaeovirus, both of which consist of non-enveloped viruses that 'inject' their genome into their host via a viral inner-membrane host plasma membrane fusion mechanism, leaving an extracellular viral capsid. Using a combination of confocal and electron microscopy, this study demonstrated for the first time that EhV-86 differs from its algal virus counterparts in two fundamental areas. Firstly, its capsid is enveloped by a lipid membrane, and secondly, EhV-86 enters its host via either an endocytotic or an envelope fusion mechanism in which an intact nucleoprotein core still encapsulated by its capsid is seen in the host cytoplasm. Real-time fluorescence microscopy showed that viral internalization and virion breakdown took place within the host on a timescale of seconds. At around 4.5 h post-infection, virus progeny were released via a budding mechanism during which EhV-86 virions became enveloped with host plasma membrane. EhV-86 therefore appears to have an infection mechanism different from that employed by other algal NCLDVs, with entry and exit strategies showing a greater analogy to animal-like NCLDVs. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/19474246/A_unicellular_algal_virus_Emiliania_huxleyi_virus_86_exploits_an_animal_like_infection_strategy_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.011635-0 DB - PRIME DP - Unbound Medicine ER -