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Life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in Movile Cave.
ISME J. 2009 Sep; 3(9):1093-104.IJ

Abstract

Microbial diversity in Movile Cave (Romania) was studied using bacterial and archaeal 16S rRNA gene sequence and functional gene analyses, including ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), soxB (sulfate thioesterase/thiohydrolase) and amoA (ammonia monooxygenase). Sulfur oxidizers from both Gammaproteobacteria and Betaproteobacteria were detected in 16S rRNA, soxB and RuBisCO gene libraries. DNA-based stable-isotope probing analyses using 13C-bicarbonate showed that Thiobacillus spp. were most active in assimilating CO2 and also implied that ammonia and nitrite oxidizers were active during incubations. Nitrosomonas spp. were detected in both 16S rRNA and amoA gene libraries from the 'heavy' DNA and sequences related to nitrite-oxidizing bacteria Nitrospira and Candidatus 'Nitrotoga' were also detected in the 'heavy' DNA, which suggests that ammonia/nitrite oxidation may be another major primary production process in this unique ecosystem. A significant number of sequences associated with known methylotrophs from the Betaproteobacteria were obtained, including Methylotenera, Methylophilus and Methylovorus, supporting the view that cycling of one-carbon compounds may be an important process within Movile Cave. Other sequences detected in the bacterial 16S rRNA clone library included Verrucomicrobia, Firmicutes, Bacteroidetes, alphaproteobacterial Rhodobacterales and gammaproteobacterial Xanthomonadales. Archaeal 16S rRNA sequences retrieved were restricted within two groups, namely the Deep-sea Hydrothermal Vent Euryarchaeota group and the Miscellaneous Crenarchaeotic group. No sequences related to known sulfur-oxidizing archaea, ammonia-oxidizing archaea, methanogens or anaerobic methane-oxidizing archaea were detected in this clone library. The results provided molecular biological evidence to support the hypothesis that Movile Cave is driven by chemolithoautotrophy, mainly through sulfur oxidation by sulfur-oxidizing bacteria and reveal that ammonia- and nitrite-oxidizing bacteria may also be major primary producers in Movile Cave.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Chen Y
Department of Biological Sciences, University of Warwick, Coventry, UK.
Wu L
No affiliation info available
Boden R
No affiliation info available
Hillebrand A
No affiliation info available
Kumaresan D
No affiliation info available
Moussard H
No affiliation info available
Baciu M
No affiliation info available
Lu Y
No affiliation info available
Colin Murrell J
No affiliation info available

MeSH

ArchaeaBacteriaBiodiversityChemoautotrophic GrowthCluster AnalysisDNA, ArchaealDNA, BacterialDNA, RibosomalGenes, rRNAMolecular Sequence DataOxidoreductasesPhylogenyQuaternary Ammonium CompoundsRNA, ArchaealRNA, BacterialRNA, Ribosomal, 16SRibulose-Bisphosphate CarboxylaseRomaniaSequence Analysis, DNASequence Homology, Nucleic AcidSoil MicrobiologySulfur

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19474813

Citation

Chen, Yin, et al. "Life Without Light: Microbial Diversity and Evidence of Sulfur- and Ammonium-based Chemolithotrophy in Movile Cave." The ISME Journal, vol. 3, no. 9, 2009, pp. 1093-104.
Chen Y, Wu L, Boden R, et al. Life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in Movile Cave. ISME J. 2009;3(9):1093-104.
Chen, Y., Wu, L., Boden, R., Hillebrand, A., Kumaresan, D., Moussard, H., Baciu, M., Lu, Y., & Colin Murrell, J. (2009). Life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in Movile Cave. The ISME Journal, 3(9), 1093-104. https://doi.org/10.1038/ismej.2009.57
Chen Y, et al. Life Without Light: Microbial Diversity and Evidence of Sulfur- and Ammonium-based Chemolithotrophy in Movile Cave. ISME J. 2009;3(9):1093-104. PubMed PMID: 19474813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in Movile Cave. AU - Chen,Yin, AU - Wu,Liqin, AU - Boden,Rich, AU - Hillebrand,Alexandra, AU - Kumaresan,Deepak, AU - Moussard,Hélène, AU - Baciu,Mihai, AU - Lu,Yahai, AU - Colin Murrell,J, Y1 - 2009/05/28/ PY - 2009/5/29/entrez PY - 2009/5/29/pubmed PY - 2009/10/16/medline SP - 1093 EP - 104 JF - The ISME journal JO - ISME J VL - 3 IS - 9 N2 - Microbial diversity in Movile Cave (Romania) was studied using bacterial and archaeal 16S rRNA gene sequence and functional gene analyses, including ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), soxB (sulfate thioesterase/thiohydrolase) and amoA (ammonia monooxygenase). Sulfur oxidizers from both Gammaproteobacteria and Betaproteobacteria were detected in 16S rRNA, soxB and RuBisCO gene libraries. DNA-based stable-isotope probing analyses using 13C-bicarbonate showed that Thiobacillus spp. were most active in assimilating CO2 and also implied that ammonia and nitrite oxidizers were active during incubations. Nitrosomonas spp. were detected in both 16S rRNA and amoA gene libraries from the 'heavy' DNA and sequences related to nitrite-oxidizing bacteria Nitrospira and Candidatus 'Nitrotoga' were also detected in the 'heavy' DNA, which suggests that ammonia/nitrite oxidation may be another major primary production process in this unique ecosystem. A significant number of sequences associated with known methylotrophs from the Betaproteobacteria were obtained, including Methylotenera, Methylophilus and Methylovorus, supporting the view that cycling of one-carbon compounds may be an important process within Movile Cave. Other sequences detected in the bacterial 16S rRNA clone library included Verrucomicrobia, Firmicutes, Bacteroidetes, alphaproteobacterial Rhodobacterales and gammaproteobacterial Xanthomonadales. Archaeal 16S rRNA sequences retrieved were restricted within two groups, namely the Deep-sea Hydrothermal Vent Euryarchaeota group and the Miscellaneous Crenarchaeotic group. No sequences related to known sulfur-oxidizing archaea, ammonia-oxidizing archaea, methanogens or anaerobic methane-oxidizing archaea were detected in this clone library. The results provided molecular biological evidence to support the hypothesis that Movile Cave is driven by chemolithoautotrophy, mainly through sulfur oxidation by sulfur-oxidizing bacteria and reveal that ammonia- and nitrite-oxidizing bacteria may also be major primary producers in Movile Cave. SN - 1751-7370 UR - https://www.unboundmedicine.com/medline/citation/19474813/Life_without_light:_microbial_diversity_and_evidence_of_sulfur__and_ammonium_based_chemolithotrophy_in_Movile_Cave_ L2 - https://doi.org/10.1038/ismej.2009.57 DB - PRIME DP - Unbound Medicine ER -