Tags

Type your tag names separated by a space and hit enter

Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance.
Biotechnol Appl Biochem. 2009 May 29; 53(Pt 3):155-64.BA

Abstract

During the fermentation of dough and the production of baker's yeast (Saccharomyces cerevisiae), cells are exposed to numerous environmental stresses (baking-associated stresses) such as freeze-thaw, high sugar concentrations, air-drying and oxidative stresses. Cellular macromolecules, including proteins, nucleic acids and membranes, are seriously damaged under stress conditions, leading to the inhibition of cell growth, cell viability and fermentation. To avoid lethal damage, yeast cells need to acquire a variety of stress-tolerant mechanisms, for example the induction of stress proteins, the accumulation of stress protectants, changes in membrane composition and repression of translation, and by regulating the corresponding gene expression via stress-triggered signal-transduction pathways. Trehalose and proline are considered to be critical stress protectants, as is glycerol. It is known that these molecules are effective for providing protection against various types of environmental stresses. Modifications of the metabolic pathways of trehalose and proline by self-cloning methods have significantly increased tolerance to baking-associated stresses. To clarify which genes are required for stress tolerance, both a comprehensive phenomics analysis and a functional genomics analysis were carried out under stress conditions that simulated those occurring during the commercial baking process. These analyses indicated that many genes are involved in stress tolerance in yeast. In particular, it was suggested that vacuolar H+-ATPase plays important roles in yeast cells under stress conditions.

Authors+Show Affiliations

National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. shimaj@affrc.go.jpNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19476439

Citation

Shima, Jun, and Hiroshi Takagi. "Stress-tolerance of Baker's-yeast (Saccharomyces Cerevisiae) Cells: Stress-protective Molecules and Genes Involved in Stress Tolerance." Biotechnology and Applied Biochemistry, vol. 53, no. Pt 3, 2009, pp. 155-64.
Shima J, Takagi H. Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance. Biotechnol Appl Biochem. 2009;53(Pt 3):155-64.
Shima, J., & Takagi, H. (2009). Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance. Biotechnology and Applied Biochemistry, 53(Pt 3), 155-64. https://doi.org/10.1042/BA20090029
Shima J, Takagi H. Stress-tolerance of Baker's-yeast (Saccharomyces Cerevisiae) Cells: Stress-protective Molecules and Genes Involved in Stress Tolerance. Biotechnol Appl Biochem. 2009 May 29;53(Pt 3):155-64. PubMed PMID: 19476439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance. AU - Shima,Jun, AU - Takagi,Hiroshi, Y1 - 2009/05/29/ PY - 2009/5/30/entrez PY - 2009/5/30/pubmed PY - 2009/7/25/medline SP - 155 EP - 64 JF - Biotechnology and applied biochemistry JO - Biotechnol Appl Biochem VL - 53 IS - Pt 3 N2 - During the fermentation of dough and the production of baker's yeast (Saccharomyces cerevisiae), cells are exposed to numerous environmental stresses (baking-associated stresses) such as freeze-thaw, high sugar concentrations, air-drying and oxidative stresses. Cellular macromolecules, including proteins, nucleic acids and membranes, are seriously damaged under stress conditions, leading to the inhibition of cell growth, cell viability and fermentation. To avoid lethal damage, yeast cells need to acquire a variety of stress-tolerant mechanisms, for example the induction of stress proteins, the accumulation of stress protectants, changes in membrane composition and repression of translation, and by regulating the corresponding gene expression via stress-triggered signal-transduction pathways. Trehalose and proline are considered to be critical stress protectants, as is glycerol. It is known that these molecules are effective for providing protection against various types of environmental stresses. Modifications of the metabolic pathways of trehalose and proline by self-cloning methods have significantly increased tolerance to baking-associated stresses. To clarify which genes are required for stress tolerance, both a comprehensive phenomics analysis and a functional genomics analysis were carried out under stress conditions that simulated those occurring during the commercial baking process. These analyses indicated that many genes are involved in stress tolerance in yeast. In particular, it was suggested that vacuolar H+-ATPase plays important roles in yeast cells under stress conditions. SN - 1470-8744 UR - https://www.unboundmedicine.com/medline/citation/19476439/Stress_tolerance_of_baker's_yeast__Saccharomyces_cerevisiae__cells:_stress_protective_molecules_and_genes_involved_in_stress_tolerance_ DB - PRIME DP - Unbound Medicine ER -