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Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies.
Ecotoxicol Environ Saf. 2009 Oct; 72(7):1842-8.EE

Abstract

The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents.

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Authors+Show Affiliations

Rocha PS
Aquatic Ecology and Toxicology Section, Heidelberg Institute of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany. rocha@zoo.uni-heidelberg.de
Luvizotto GL
No affiliation info available
Kosmehl T
No affiliation info available
Böttcher M
No affiliation info available
Storch V
No affiliation info available
Braunbeck T
No affiliation info available
Hollert H
No affiliation info available

MeSH

AnimalsBrazilCell LineComet AssayDNA DamageEnvironmental MonitoringErythrocytesFibroblastsGeologic SedimentsLiverMicronuclei, Chromosome-DefectiveMicronucleus TestsOncorhynchus mykissRiversTilapiaWater Pollutants, Chemical

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19477010

Citation

Rocha, Paula Suares, et al. "Sediment Genotoxicity in the Tietê River (São Paulo, Brazil): in Vitro Comet Assay Versus in Situ Micronucleus Assay Studies." Ecotoxicology and Environmental Safety, vol. 72, no. 7, 2009, pp. 1842-8.
Rocha PS, Luvizotto GL, Kosmehl T, et al. Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies. Ecotoxicol Environ Saf. 2009;72(7):1842-8.
Rocha, P. S., Luvizotto, G. L., Kosmehl, T., Böttcher, M., Storch, V., Braunbeck, T., & Hollert, H. (2009). Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies. Ecotoxicology and Environmental Safety, 72(7), 1842-8. https://doi.org/10.1016/j.ecoenv.2009.04.013
Rocha PS, et al. Sediment Genotoxicity in the Tietê River (São Paulo, Brazil): in Vitro Comet Assay Versus in Situ Micronucleus Assay Studies. Ecotoxicol Environ Saf. 2009;72(7):1842-8. PubMed PMID: 19477010.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies. AU - Rocha,Paula Suares, AU - Luvizotto,George Luiz, AU - Kosmehl,Thomas, AU - Böttcher,Melanie, AU - Storch,Volker, AU - Braunbeck,Thomas, AU - Hollert,Henner, Y1 - 2009/05/23/ PY - 2008/07/30/received PY - 2009/04/06/revised PY - 2009/04/13/accepted PY - 2009/5/30/entrez PY - 2009/5/30/pubmed PY - 2009/12/16/medline SP - 1842 EP - 8 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 72 IS - 7 N2 - The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/19477010/Sediment_genotoxicity_in_the_Tietê_River__São_Paulo_Brazil_:_in_vitro_comet_assay_versus_in_situ_micronucleus_assay_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(09)00090-6 DB - PRIME DP - Unbound Medicine ER -