Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies.Ecotoxicol Environ Saf. 2009 Oct; 72(7):1842-8.EE
Abstract
The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents.
Links
MeSH
Pub Type(s)
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
19477010
Citation
Rocha, Paula Suares, et al. "Sediment Genotoxicity in the Tietê River (São Paulo, Brazil): in Vitro Comet Assay Versus in Situ Micronucleus Assay Studies." Ecotoxicology and Environmental Safety, vol. 72, no. 7, 2009, pp. 1842-8.
Rocha PS, Luvizotto GL, Kosmehl T, et al. Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies. Ecotoxicol Environ Saf. 2009;72(7):1842-8.
Rocha, P. S., Luvizotto, G. L., Kosmehl, T., Böttcher, M., Storch, V., Braunbeck, T., & Hollert, H. (2009). Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies. Ecotoxicology and Environmental Safety, 72(7), 1842-8. https://doi.org/10.1016/j.ecoenv.2009.04.013
Rocha PS, et al. Sediment Genotoxicity in the Tietê River (São Paulo, Brazil): in Vitro Comet Assay Versus in Situ Micronucleus Assay Studies. Ecotoxicol Environ Saf. 2009;72(7):1842-8. PubMed PMID: 19477010.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies.
AU - Rocha,Paula Suares,
AU - Luvizotto,George Luiz,
AU - Kosmehl,Thomas,
AU - Böttcher,Melanie,
AU - Storch,Volker,
AU - Braunbeck,Thomas,
AU - Hollert,Henner,
Y1 - 2009/05/23/
PY - 2008/07/30/received
PY - 2009/04/06/revised
PY - 2009/04/13/accepted
PY - 2009/5/30/entrez
PY - 2009/5/30/pubmed
PY - 2009/12/16/medline
SP - 1842
EP - 8
JF - Ecotoxicology and environmental safety
JO - Ecotoxicol Environ Saf
VL - 72
IS - 7
N2 - The in vitro comet assay with the permanent fish cell line RTL-W1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (Oreochromis niloticus) were used to detect genotoxicity in Tietê River sediments (São Paulo, Brazil). Either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Discharges from São Paulo city have major impact on genotoxic effects by sediment-bound contaminants; however, overall genotoxicity decreases downstream. The high genotoxic burden of the Tietê River warrants measures to reduce the input of toxic effluents.
SN - 1090-2414
UR - https://www.unboundmedicine.com/medline/citation/19477010/Sediment_genotoxicity_in_the_Tietê_River__São_Paulo_Brazil_:_in_vitro_comet_assay_versus_in_situ_micronucleus_assay_studies_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(09)00090-6
DB - PRIME
DP - Unbound Medicine
ER -