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Salacia oblonga extract increases glucose transporter 4-mediated glucose uptake in L6 rat myotubes: role of mangiferin.
Clin Nutr. 2009 Oct; 28(5):565-74.CN

Abstract

BACKGROUND AND AIMS

To evaluate if the antidiabetic properties of Salacia oblonga extract are mediated not only by inhibiting intestinal alpha-glycosidases but also by enhancing glucose transport in muscle and adipose cells.

METHODS

S. oblonga extract effects on 2-deoxy-D-glucose uptake were assayed in muscle L6-myotubes and 3T3-adipocytes. In L6-myotubes, the amount and translocation of glucose transporters were assayed. A fractionation of the extract was carried out to identify the active compounds. Furthermore, we analyzed the phosphorylation status of key components of signaling pathways that are involved in the molecular mechanisms regulating glucose uptake.

RESULTS

S. oblonga extract increased 2-deoxy-D-glucose uptake by 50% in L6-myotubes and 3T3-adipocytes. In L6-myotubes, the extract increased up to a 100% the GLUT4 content, activating GLUT4 promoter transcription and its translocation to the plasma membrane. Mangiferin was identified as the bioactive compound. Furthermore, mangiferin effects were concomitant with the phosphorylation of 5'-AMP-activated protein kinase without the activation of PKB/Akt. The effect of mangiferin on 2-deoxy-D-glucose uptake was blocked by GW9662, an irreversible PPAR-gamma antagonist.

CONCLUSIONS

S. oblonga extract and mangiferin may exert their antidiabetic effect by increasing GLUT4 expression and translocation in muscle cells. These effects are probably mediated through two independent pathways that are related to 5'-AMP-activated protein kinase and PPAR-gamma.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Campus de Cartuja sn, E-18071 Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19477051

Citation

Girón, María Dolores, et al. "Salacia Oblonga Extract Increases Glucose Transporter 4-mediated Glucose Uptake in L6 Rat Myotubes: Role of Mangiferin." Clinical Nutrition (Edinburgh, Scotland), vol. 28, no. 5, 2009, pp. 565-74.
Girón MD, Sevillano N, Salto R, et al. Salacia oblonga extract increases glucose transporter 4-mediated glucose uptake in L6 rat myotubes: role of mangiferin. Clin Nutr. 2009;28(5):565-74.
Girón, M. D., Sevillano, N., Salto, R., Haidour, A., Manzano, M., Jiménez, M. L., Rueda, R., & López-Pedrosa, J. M. (2009). Salacia oblonga extract increases glucose transporter 4-mediated glucose uptake in L6 rat myotubes: role of mangiferin. Clinical Nutrition (Edinburgh, Scotland), 28(5), 565-74. https://doi.org/10.1016/j.clnu.2009.04.018
Girón MD, et al. Salacia Oblonga Extract Increases Glucose Transporter 4-mediated Glucose Uptake in L6 Rat Myotubes: Role of Mangiferin. Clin Nutr. 2009;28(5):565-74. PubMed PMID: 19477051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salacia oblonga extract increases glucose transporter 4-mediated glucose uptake in L6 rat myotubes: role of mangiferin. AU - Girón,María Dolores, AU - Sevillano,Natalia, AU - Salto,Rafael, AU - Haidour,Ali, AU - Manzano,Manuel, AU - Jiménez,M L, AU - Rueda,Ricardo, AU - López-Pedrosa,José M, Y1 - 2009/05/23/ PY - 2008/09/19/received PY - 2009/04/02/revised PY - 2009/04/10/accepted PY - 2009/5/30/entrez PY - 2009/5/30/pubmed PY - 2010/2/2/medline SP - 565 EP - 74 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 28 IS - 5 N2 - BACKGROUND AND AIMS: To evaluate if the antidiabetic properties of Salacia oblonga extract are mediated not only by inhibiting intestinal alpha-glycosidases but also by enhancing glucose transport in muscle and adipose cells. METHODS: S. oblonga extract effects on 2-deoxy-D-glucose uptake were assayed in muscle L6-myotubes and 3T3-adipocytes. In L6-myotubes, the amount and translocation of glucose transporters were assayed. A fractionation of the extract was carried out to identify the active compounds. Furthermore, we analyzed the phosphorylation status of key components of signaling pathways that are involved in the molecular mechanisms regulating glucose uptake. RESULTS: S. oblonga extract increased 2-deoxy-D-glucose uptake by 50% in L6-myotubes and 3T3-adipocytes. In L6-myotubes, the extract increased up to a 100% the GLUT4 content, activating GLUT4 promoter transcription and its translocation to the plasma membrane. Mangiferin was identified as the bioactive compound. Furthermore, mangiferin effects were concomitant with the phosphorylation of 5'-AMP-activated protein kinase without the activation of PKB/Akt. The effect of mangiferin on 2-deoxy-D-glucose uptake was blocked by GW9662, an irreversible PPAR-gamma antagonist. CONCLUSIONS: S. oblonga extract and mangiferin may exert their antidiabetic effect by increasing GLUT4 expression and translocation in muscle cells. These effects are probably mediated through two independent pathways that are related to 5'-AMP-activated protein kinase and PPAR-gamma. SN - 1532-1983 UR - https://www.unboundmedicine.com/medline/citation/19477051/Salacia_oblonga_extract_increases_glucose_transporter_4_mediated_glucose_uptake_in_L6_rat_myotubes:_role_of_mangiferin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261-5614(09)00089-2 DB - PRIME DP - Unbound Medicine ER -