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Distribution of human papillomavirus genotypes in women with cervical cancer in Slovenia.
Eur J Obstet Gynecol Reprod Biol. 2009 Aug; 145(2):184-8.EJ

Abstract

OBJECTIVE

The purpose of the present study was to establish the distribution of human papillomavirus (HPV) genotypes in a representative population of women with cervical cancer in Slovenia in order to contribute to the lacking data on HPV in cervical cancer and to assess the potential local benefit of future prophylactic HPV vaccination.

STUDY DESIGN

A total of 284 samples of cervical cancer were analyzed including archival samples, cervical scrapes and fresh tissue samples. Polymerase chain reaction with GP5+/GP6+ primers was performed in all samples for HPV deoxyribonucleic acid (DNA) detection. All GP5+/GP6+ negative samples were additionally tested using CPI/CPIIg primers and INNO-LiPA HPV genotyping assay.

RESULTS

After exclusion of 6 samples with unsuccessful amplification of beta-globin gene, 262 of 278 cervical cancer samples (94.2%) were HPV DNA positive. HPV genotypes found in the decreasing order of frequency were: HPV 16 (64.9%), HPV 18 (12.2%), HPV 33 (4.7%), HPV 45 (4.1%), followed by HPV 31, 51, 58, 59, 35, 52, 73 and 82 (3.5-0.2%). HPV positive samples were more frequent among squamous cell carcinomas than among adenocarcinomas/adenosquamous carcinomas (95.8% versus 85.5%; P=0.003). HPV 16 was more frequently found in squamous cell carcinomas than in adenocarcinomas/adenosquamous carcinomas (69.9% versus 37.5%; P<0.001), while the opposite was true for HPV 18 (6% versus 41.7%; P<0.001).

CONCLUSION

Prophylactic HPV vaccination with currently available vaccines could prevent up to 77.1% of cervical cancer in Slovenia, which is caused by HPV 16 or HPV 18.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Ljubljana, Slovenia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19477061

Citation

Jancar, Nina, et al. "Distribution of Human Papillomavirus Genotypes in Women With Cervical Cancer in Slovenia." European Journal of Obstetrics, Gynecology, and Reproductive Biology, vol. 145, no. 2, 2009, pp. 184-8.
Jancar N, Kocjan BJ, Poljak M, et al. Distribution of human papillomavirus genotypes in women with cervical cancer in Slovenia. Eur J Obstet Gynecol Reprod Biol. 2009;145(2):184-8.
Jancar, N., Kocjan, B. J., Poljak, M., Lunar, M. M., & Bokal, E. V. (2009). Distribution of human papillomavirus genotypes in women with cervical cancer in Slovenia. European Journal of Obstetrics, Gynecology, and Reproductive Biology, 145(2), 184-8. https://doi.org/10.1016/j.ejogrb.2009.04.030
Jancar N, et al. Distribution of Human Papillomavirus Genotypes in Women With Cervical Cancer in Slovenia. Eur J Obstet Gynecol Reprod Biol. 2009;145(2):184-8. PubMed PMID: 19477061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of human papillomavirus genotypes in women with cervical cancer in Slovenia. AU - Jancar,Nina, AU - Kocjan,Bostjan J, AU - Poljak,Mario, AU - Lunar,Maja M, AU - Bokal,Eda Vrtacnik, Y1 - 2009/05/23/ PY - 2009/01/12/received PY - 2009/03/23/revised PY - 2009/04/27/accepted PY - 2009/5/30/entrez PY - 2009/5/30/pubmed PY - 2009/10/23/medline SP - 184 EP - 8 JF - European journal of obstetrics, gynecology, and reproductive biology JO - Eur J Obstet Gynecol Reprod Biol VL - 145 IS - 2 N2 - OBJECTIVE: The purpose of the present study was to establish the distribution of human papillomavirus (HPV) genotypes in a representative population of women with cervical cancer in Slovenia in order to contribute to the lacking data on HPV in cervical cancer and to assess the potential local benefit of future prophylactic HPV vaccination. STUDY DESIGN: A total of 284 samples of cervical cancer were analyzed including archival samples, cervical scrapes and fresh tissue samples. Polymerase chain reaction with GP5+/GP6+ primers was performed in all samples for HPV deoxyribonucleic acid (DNA) detection. All GP5+/GP6+ negative samples were additionally tested using CPI/CPIIg primers and INNO-LiPA HPV genotyping assay. RESULTS: After exclusion of 6 samples with unsuccessful amplification of beta-globin gene, 262 of 278 cervical cancer samples (94.2%) were HPV DNA positive. HPV genotypes found in the decreasing order of frequency were: HPV 16 (64.9%), HPV 18 (12.2%), HPV 33 (4.7%), HPV 45 (4.1%), followed by HPV 31, 51, 58, 59, 35, 52, 73 and 82 (3.5-0.2%). HPV positive samples were more frequent among squamous cell carcinomas than among adenocarcinomas/adenosquamous carcinomas (95.8% versus 85.5%; P=0.003). HPV 16 was more frequently found in squamous cell carcinomas than in adenocarcinomas/adenosquamous carcinomas (69.9% versus 37.5%; P<0.001), while the opposite was true for HPV 18 (6% versus 41.7%; P<0.001). CONCLUSION: Prophylactic HPV vaccination with currently available vaccines could prevent up to 77.1% of cervical cancer in Slovenia, which is caused by HPV 16 or HPV 18. SN - 1872-7654 UR - https://www.unboundmedicine.com/medline/citation/19477061/Distribution_of_human_papillomavirus_genotypes_in_women_with_cervical_cancer_in_Slovenia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301-2115(09)00305-4 DB - PRIME DP - Unbound Medicine ER -