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Ovarian stimulation with FSH in the rat reduces proestrous GnRH-dependent LH secretion through a dual mechanism: inhibition of LH synthesis and release.
Neurosci Lett. 2009 Sep 04; 460(3):219-22.NL

Abstract

Administration of human follicle-stimulating hormone (hFSH) to female rats during diestrus phase attenuates the spontaneous luteinizing hormone (LH) surge in proestrous afternoon. The inhibition of LH secretion is associated with a decreased pituitary LH content in intact, but not in ovariectomized rats injected with 25mug estradiol benzoate (EB). This suggests that the mechanism of action of the putative non-steroidal ovarian bioactive FSH-dependent gonadodotropin surge attenuating factor (GnSAF) might, in addition, involve a reduction in LH synthesis. The present experiments studied, in proestrous pituitaries, the effects of different doses of hFSH, with or without EB on: (i) basal and GnRH-stimulated LH release and GnRH self-priming, (ii) LHbeta mRNA values, and (iii) LH content. Results showed that bioactive GnSAF reduced mainly GnRH self-priming, but also GnRH-stimulated LH secretion and pituitary LH content in a dose-dependent manner. GnSAF had no effect on basal LH secretion or pituitary LHbeta mRNA values. EB increased pituitary sensitivity to GnRH in controls, and overcame the inhibitory effects of GnSAF after low doses of hFSH but not after 10IU of hFSH. In contrast with the sensitizing action of EB on LH secretion, EB had no effect on pituitary LHbeta mRNA content or LH protein. It is concluded that the putative GnSAF blunted the LH surge by reducing LH synthesis at post-transcriptional level and antagonizing the GnRH-dependent LH secretion and the sensitizing effect of estradiol to GnRH.

Authors+Show Affiliations

Department of Cell Biology, Physiology and Immunology, University of Córdoba, 14004 Córdoba, Spain. anagordonbc@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19477224

Citation

Gordon, Ana, et al. "Ovarian Stimulation With FSH in the Rat Reduces Proestrous GnRH-dependent LH Secretion Through a Dual Mechanism: Inhibition of LH Synthesis and Release." Neuroscience Letters, vol. 460, no. 3, 2009, pp. 219-22.
Gordon A, Garrido-Gracia JC, Aguilar R, et al. Ovarian stimulation with FSH in the rat reduces proestrous GnRH-dependent LH secretion through a dual mechanism: inhibition of LH synthesis and release. Neurosci Lett. 2009;460(3):219-22.
Gordon, A., Garrido-Gracia, J. C., Aguilar, R., & Sánchez-Criado, J. E. (2009). Ovarian stimulation with FSH in the rat reduces proestrous GnRH-dependent LH secretion through a dual mechanism: inhibition of LH synthesis and release. Neuroscience Letters, 460(3), 219-22. https://doi.org/10.1016/j.neulet.2009.05.055
Gordon A, et al. Ovarian Stimulation With FSH in the Rat Reduces Proestrous GnRH-dependent LH Secretion Through a Dual Mechanism: Inhibition of LH Synthesis and Release. Neurosci Lett. 2009 Sep 4;460(3):219-22. PubMed PMID: 19477224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ovarian stimulation with FSH in the rat reduces proestrous GnRH-dependent LH secretion through a dual mechanism: inhibition of LH synthesis and release. AU - Gordon,Ana, AU - Garrido-Gracia,Jose C, AU - Aguilar,Rafaela, AU - Sánchez-Criado,José E, Y1 - 2009/05/27/ PY - 2009/03/11/received PY - 2009/04/24/revised PY - 2009/05/19/accepted PY - 2009/5/30/entrez PY - 2009/5/30/pubmed PY - 2009/8/26/medline SP - 219 EP - 22 JF - Neuroscience letters JO - Neurosci Lett VL - 460 IS - 3 N2 - Administration of human follicle-stimulating hormone (hFSH) to female rats during diestrus phase attenuates the spontaneous luteinizing hormone (LH) surge in proestrous afternoon. The inhibition of LH secretion is associated with a decreased pituitary LH content in intact, but not in ovariectomized rats injected with 25mug estradiol benzoate (EB). This suggests that the mechanism of action of the putative non-steroidal ovarian bioactive FSH-dependent gonadodotropin surge attenuating factor (GnSAF) might, in addition, involve a reduction in LH synthesis. The present experiments studied, in proestrous pituitaries, the effects of different doses of hFSH, with or without EB on: (i) basal and GnRH-stimulated LH release and GnRH self-priming, (ii) LHbeta mRNA values, and (iii) LH content. Results showed that bioactive GnSAF reduced mainly GnRH self-priming, but also GnRH-stimulated LH secretion and pituitary LH content in a dose-dependent manner. GnSAF had no effect on basal LH secretion or pituitary LHbeta mRNA values. EB increased pituitary sensitivity to GnRH in controls, and overcame the inhibitory effects of GnSAF after low doses of hFSH but not after 10IU of hFSH. In contrast with the sensitizing action of EB on LH secretion, EB had no effect on pituitary LHbeta mRNA content or LH protein. It is concluded that the putative GnSAF blunted the LH surge by reducing LH synthesis at post-transcriptional level and antagonizing the GnRH-dependent LH secretion and the sensitizing effect of estradiol to GnRH. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/19477224/Ovarian_stimulation_with_FSH_in_the_rat_reduces_proestrous_GnRH_dependent_LH_secretion_through_a_dual_mechanism:_inhibition_of_LH_synthesis_and_release_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(09)00706-X DB - PRIME DP - Unbound Medicine ER -