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Design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties.

Abstract

The aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of Sechium edule fluid extract and to obtain a pharmaceutical formulation with them. The extract exhibited antimicrobial activity against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Serratia marcescens, Morganella morganii, Acinetobacter baumannii, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Candida spp. and Aspergillus spp. isolated from clinical samples from two hospitals of Tucuman, Argentina. Non-toxicity and mutagenicity on both Salmonella typhimurium TA98 and TA 100 strains until 100 microg/plate were observed. A hydrogel with carbopol acrylic acid polymer containing S. edule fluid extract as antibacterial, antimycotic and antioxidant agent was obtained. Microbiological, physical and functional stability of pharmaceutical formulation conserved at room temperature for 1 year were determined. Addition of antioxidant preservatives to store the pharmaceutical formulation was not necessary. The semisolid system showed antimicrobial activity against all gram positive and gram negative bacteria and fungi assayed. The minimal inhibitory concentration (MIC) values ranged from 20 to 800 microg/mL. Its activity was compared with a pharmaceutical formulation containing commercial antibiotic and antifungal. A pseudoplastic behavior and positive thixotropy were observed. Our current finding shows an antimicrobial activity of hydrogel containing S. edule extract on a large range of gram negative and gram positive multi-resistant bacteria and fungi. This topical formulation may be used as antimycotic and as antibacterial in cutaneous infections.

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  • Authors+Show Affiliations

    ,

    Instituto de Farmacia Dr. Adolfo Rovelli, Universidad Nacional de Tucumán, Ayacucho 471, T4000INI, San Miguel de Tucumán, Tucumán, Argentina.

    , ,

    Source

    International journal of pharmaceutics 378:1-2 2009 Aug 13 pg 51-8

    MeSH

    Acrylates
    Anti-Bacterial Agents
    Antifungal Agents
    Antioxidants
    Argentina
    Cucurbitaceae
    Drug Carriers
    Drug Stability
    Drug Storage
    Humans
    Hydrogels
    Microbial Sensitivity Tests
    Mutagenicity Tests
    Plant Extracts
    Quality Control
    Viscosity

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19477252

    Citation

    Ordoñez, Adriana A L., et al. "Design and Quality Control of a Pharmaceutical Formulation Containing Natural Products With Antibacterial, Antifungal and Antioxidant Properties." International Journal of Pharmaceutics, vol. 378, no. 1-2, 2009, pp. 51-8.
    Ordoñez AA, Ordoñez RM, Zampini IC, et al. Design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties. Int J Pharm. 2009;378(1-2):51-8.
    Ordoñez, A. A., Ordoñez, R. M., Zampini, I. C., & Isla, M. I. (2009). Design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties. International Journal of Pharmaceutics, 378(1-2), pp. 51-8. doi:10.1016/j.ijpharm.2009.05.040.
    Ordoñez AA, et al. Design and Quality Control of a Pharmaceutical Formulation Containing Natural Products With Antibacterial, Antifungal and Antioxidant Properties. Int J Pharm. 2009 Aug 13;378(1-2):51-8. PubMed PMID: 19477252.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties. AU - Ordoñez,Adriana A L, AU - Ordoñez,Roxana M, AU - Zampini,Iris C, AU - Isla,María I, Y1 - 2009/05/27/ PY - 2009/02/10/received PY - 2009/05/16/revised PY - 2009/05/19/accepted PY - 2009/5/30/entrez PY - 2009/5/30/pubmed PY - 2009/12/16/medline SP - 51 EP - 8 JF - International journal of pharmaceutics JO - Int J Pharm VL - 378 IS - 1-2 N2 - The aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of Sechium edule fluid extract and to obtain a pharmaceutical formulation with them. The extract exhibited antimicrobial activity against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Serratia marcescens, Morganella morganii, Acinetobacter baumannii, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Candida spp. and Aspergillus spp. isolated from clinical samples from two hospitals of Tucuman, Argentina. Non-toxicity and mutagenicity on both Salmonella typhimurium TA98 and TA 100 strains until 100 microg/plate were observed. A hydrogel with carbopol acrylic acid polymer containing S. edule fluid extract as antibacterial, antimycotic and antioxidant agent was obtained. Microbiological, physical and functional stability of pharmaceutical formulation conserved at room temperature for 1 year were determined. Addition of antioxidant preservatives to store the pharmaceutical formulation was not necessary. The semisolid system showed antimicrobial activity against all gram positive and gram negative bacteria and fungi assayed. The minimal inhibitory concentration (MIC) values ranged from 20 to 800 microg/mL. Its activity was compared with a pharmaceutical formulation containing commercial antibiotic and antifungal. A pseudoplastic behavior and positive thixotropy were observed. Our current finding shows an antimicrobial activity of hydrogel containing S. edule extract on a large range of gram negative and gram positive multi-resistant bacteria and fungi. This topical formulation may be used as antimycotic and as antibacterial in cutaneous infections. SN - 1873-3476 UR - https://www.unboundmedicine.com/medline/citation/19477252/Design_and_quality_control_of_a_pharmaceutical_formulation_containing_natural_products_with_antibacterial_antifungal_and_antioxidant_properties_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5173(09)00345-7 DB - PRIME DP - Unbound Medicine ER -