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TNF-alpha expression in gastric mucosa of individuals infected with different virulent Helicobacter pylori strains.
Med Sci Monit. 2009 Jun; 15(6):BR166-71.MS

Abstract

BACKGROUND

Helicobacter pylori colonizes the human gastric mucosa, leading to chronic superficial gastritis and in some cases to peptic ulceration, gastric adenocarcinoma, or gastric lymphoma. It has been postulated that the clinical outcome depends on differences in H. pylori strain virulence as well as on individual factors of the host. Thus, we aimed to assess the relation between H. pylori cagA/vacA genotypes and the TNF-alpha gene expression in gastric mucosa specimens from patients with chronic gastritis.

MATERIAL/METHODS

This study was conducted with gastric mucosa samples obtained during gastroendoscopy from 43 H. pylori-infected individuals with chronic gastritis. The presence of ureA and cagA genes and vacA allele combinations were analyzed in isolated DNA by the polymerase chain reaction method. Isolates of RNA were used for cDNA synthesis by reverse transcription. Synthesized cDNA was used to determine the TNF-alpha gene expression level by quantitative real-time polymerase chain reaction assay.

RESULTS

The cagA gene was detected in 67.44% of H. pylori strains. Five vacA alleles in the tested H. pylori strains were detected: s1a/m1 (18.60%), s1a/m2 (23.26%), s1a/m3 (18.60%), s1b/m2 (6.98%), and s2m2 (32.56%). There were no significant differences in TNF-alpha gene expression in strains expressing cagA versus those not expressing this gene, and no significant differences among strains with different vacA alleles.

CONCLUSIONS

TNF-alpha gene expression in the gastric mucosa of H. pylori-infected patients appears to be independent of H. pylori cagA/vacA genotype.

Authors+Show Affiliations

Department of General Biology, Medical University of Silesia, Zabrze, Poland. marzenazz@poczta.onet.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19478694

Citation

Zalewska-Ziob, Marzena, et al. "TNF-alpha Expression in Gastric Mucosa of Individuals Infected With Different Virulent Helicobacter Pylori Strains." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 15, no. 6, 2009, pp. BR166-71.
Zalewska-Ziob M, Adamek B, Strzelczyk JK, et al. TNF-alpha expression in gastric mucosa of individuals infected with different virulent Helicobacter pylori strains. Med Sci Monit. 2009;15(6):BR166-71.
Zalewska-Ziob, M., Adamek, B., Strzelczyk, J. K., Gawron, K., Jarzab, B., Gubała, E., Kula, D., Krakowczyk, Ł., Sieroń, A., & Wiczkowski, A. (2009). TNF-alpha expression in gastric mucosa of individuals infected with different virulent Helicobacter pylori strains. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 15(6), BR166-71.
Zalewska-Ziob M, et al. TNF-alpha Expression in Gastric Mucosa of Individuals Infected With Different Virulent Helicobacter Pylori Strains. Med Sci Monit. 2009;15(6):BR166-71. PubMed PMID: 19478694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TNF-alpha expression in gastric mucosa of individuals infected with different virulent Helicobacter pylori strains. AU - Zalewska-Ziob,Marzena, AU - Adamek,Brygida, AU - Strzelczyk,Joanna Katarzyna, AU - Gawron,Katarzyna, AU - Jarzab,Barbara, AU - Gubała,Elzbieta, AU - Kula,Dorota, AU - Krakowczyk,Łukasz, AU - Sieroń,Aleksander, AU - Wiczkowski,Andrzej, PY - 2009/5/30/entrez PY - 2009/5/30/pubmed PY - 2009/7/31/medline SP - BR166 EP - 71 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 15 IS - 6 N2 - BACKGROUND: Helicobacter pylori colonizes the human gastric mucosa, leading to chronic superficial gastritis and in some cases to peptic ulceration, gastric adenocarcinoma, or gastric lymphoma. It has been postulated that the clinical outcome depends on differences in H. pylori strain virulence as well as on individual factors of the host. Thus, we aimed to assess the relation between H. pylori cagA/vacA genotypes and the TNF-alpha gene expression in gastric mucosa specimens from patients with chronic gastritis. MATERIAL/METHODS: This study was conducted with gastric mucosa samples obtained during gastroendoscopy from 43 H. pylori-infected individuals with chronic gastritis. The presence of ureA and cagA genes and vacA allele combinations were analyzed in isolated DNA by the polymerase chain reaction method. Isolates of RNA were used for cDNA synthesis by reverse transcription. Synthesized cDNA was used to determine the TNF-alpha gene expression level by quantitative real-time polymerase chain reaction assay. RESULTS: The cagA gene was detected in 67.44% of H. pylori strains. Five vacA alleles in the tested H. pylori strains were detected: s1a/m1 (18.60%), s1a/m2 (23.26%), s1a/m3 (18.60%), s1b/m2 (6.98%), and s2m2 (32.56%). There were no significant differences in TNF-alpha gene expression in strains expressing cagA versus those not expressing this gene, and no significant differences among strains with different vacA alleles. CONCLUSIONS: TNF-alpha gene expression in the gastric mucosa of H. pylori-infected patients appears to be independent of H. pylori cagA/vacA genotype. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/19478694/TNF_alpha_expression_in_gastric_mucosa_of_individuals_infected_with_different_virulent_Helicobacter_pylori_strains_ L2 - https://www.medscimonit.com/download/index/idArt/869672 DB - PRIME DP - Unbound Medicine ER -