Tags

Type your tag names separated by a space and hit enter

Cathepsin L-like cysteine proteinase (DcCathL) from Delia coarctata (wheat bulb fly): basis of insecticidal activity.
Insect Biochem Mol Biol. 2009 Aug; 39(8):535-46.IB

Abstract

A cDNA encoding a cathepsin L-like cysteine proteinase (DcCathL) was prepared from gut tissue of larvae of wheat bulb fly (Delia coarctata: Diptera). The predicted protein is a homologue of the product of Drosophila melanogaster gene Cp-1 (CG6692), and is similar to a sub-family of cysteine proteinases found in other insects which have roles in tissue remodelling during development, and moulting. Recombinant DcCathL was produced using the yeast Pichia pastoris as expression host, and showed hydrolytic activity in vitro towards the synthetic substrate Z-Phe-Arg-AMC with a pH optimum of 4.5. DcCathL was insecticidal to lepidopteran larvae when injected into haemolymph, causing mortality that was accompanied by systemic melanisation, suggesting that DcCathL was affecting the immune-related proteolytic activation cascade leading to production of active phenoloxidase. This process is normally negatively regulated by serpins in the haemolymph. Recombinant serpins from cabbage moth (Mamestra brassicae) did not inhibit DcCathL, and were susceptible to degradation by the enzyme in vitro in buffer and extracted haemolymph. When M. brassicae larvae were co-injected with a lethal dose of DcCathL and exogenous recombinant serpins, no mortality or systemic melanisation was observed, suggesting that the insecticidal effects of DcCathL in vivo result from degradation of endogenous serpins.

Authors+Show Affiliations

School of Biological and Biomedical Sciences, Durham University, South Road, Durham, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19481148

Citation

Pyati, Prashant S., et al. "Cathepsin L-like Cysteine Proteinase (DcCathL) From Delia Coarctata (wheat Bulb Fly): Basis of Insecticidal Activity." Insect Biochemistry and Molecular Biology, vol. 39, no. 8, 2009, pp. 535-46.
Pyati PS, Bell HA, Fitches E, et al. Cathepsin L-like cysteine proteinase (DcCathL) from Delia coarctata (wheat bulb fly): basis of insecticidal activity. Insect Biochem Mol Biol. 2009;39(8):535-46.
Pyati, P. S., Bell, H. A., Fitches, E., Price, D. R., Gatehouse, A. M., & Gatehouse, J. A. (2009). Cathepsin L-like cysteine proteinase (DcCathL) from Delia coarctata (wheat bulb fly): basis of insecticidal activity. Insect Biochemistry and Molecular Biology, 39(8), 535-46. https://doi.org/10.1016/j.ibmb.2009.05.003
Pyati PS, et al. Cathepsin L-like Cysteine Proteinase (DcCathL) From Delia Coarctata (wheat Bulb Fly): Basis of Insecticidal Activity. Insect Biochem Mol Biol. 2009;39(8):535-46. PubMed PMID: 19481148.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cathepsin L-like cysteine proteinase (DcCathL) from Delia coarctata (wheat bulb fly): basis of insecticidal activity. AU - Pyati,Prashant S, AU - Bell,Howard A, AU - Fitches,Elaine, AU - Price,Daniel R G, AU - Gatehouse,Angharad M R, AU - Gatehouse,John A, Y1 - 2009/05/27/ PY - 2009/03/06/received PY - 2009/05/05/revised PY - 2009/05/16/accepted PY - 2009/6/2/entrez PY - 2009/6/2/pubmed PY - 2009/9/25/medline SP - 535 EP - 46 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 39 IS - 8 N2 - A cDNA encoding a cathepsin L-like cysteine proteinase (DcCathL) was prepared from gut tissue of larvae of wheat bulb fly (Delia coarctata: Diptera). The predicted protein is a homologue of the product of Drosophila melanogaster gene Cp-1 (CG6692), and is similar to a sub-family of cysteine proteinases found in other insects which have roles in tissue remodelling during development, and moulting. Recombinant DcCathL was produced using the yeast Pichia pastoris as expression host, and showed hydrolytic activity in vitro towards the synthetic substrate Z-Phe-Arg-AMC with a pH optimum of 4.5. DcCathL was insecticidal to lepidopteran larvae when injected into haemolymph, causing mortality that was accompanied by systemic melanisation, suggesting that DcCathL was affecting the immune-related proteolytic activation cascade leading to production of active phenoloxidase. This process is normally negatively regulated by serpins in the haemolymph. Recombinant serpins from cabbage moth (Mamestra brassicae) did not inhibit DcCathL, and were susceptible to degradation by the enzyme in vitro in buffer and extracted haemolymph. When M. brassicae larvae were co-injected with a lethal dose of DcCathL and exogenous recombinant serpins, no mortality or systemic melanisation was observed, suggesting that the insecticidal effects of DcCathL in vivo result from degradation of endogenous serpins. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/19481148/Cathepsin_L_like_cysteine_proteinase__DcCathL__from_Delia_coarctata__wheat_bulb_fly_:_basis_of_insecticidal_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(09)00080-0 DB - PRIME DP - Unbound Medicine ER -