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In situ derivatization hollow fibre liquid-phase microextraction for the determination of biogenic amines in food samples.
J Chromatogr A. 2009 Jul 03; 1216(27):5165-70.JC

Abstract

Hollow fibre liquid-phase microextraction with in situ derivatization using dansyl chloride has been successfully developed for the high-performance liquid chromatography-ultraviolet (HPLC-UV) determination of the biogenic amines (tryptamine, putrescine, cadaverine, histamine, tyramine, spermidine) in food samples. Parameters affecting the performance of the in situ derivatization process such as type of extraction solvent, temperature, extraction time, stirring speed and salt addition were studied and optimized. Under the optimized conditions (extraction solvent, dihexyl ether; acceptor phase, 0.1M HCl; extraction time, 30 min; extraction temperature, 26 degrees C; without addition of salt), enrichment factors varying from 47 to 456 were achieved. Good linearity of the analytes was obtained over a concentration range of 0.1-5 microg mL(-1) (with correlation coefficients of 0.9901-0.9974). The limits of detection and quantification based on a signal-to-noise ratio of 3-10, ranged from 0.0075 to 0.030 microg mL(-1) and 0.03 to 0.10 microg mL(-1), respectively. The relative standard deviations based on the peak areas for six replicate analysis of water spiked with 0.5 microg mL(-1) of each biogenic amine were lower than 7.5%. The method was successfully applied to shrimp sauce and tomato ketchup samples, offering an interesting alternative to liquid-liquid extraction and solid phase extraction for the analysis of biogenic amines in food samples.

Authors+Show Affiliations

School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19481215

Citation

Saaid, Mardiana, et al. "In Situ Derivatization Hollow Fibre Liquid-phase Microextraction for the Determination of Biogenic Amines in Food Samples." Journal of Chromatography. A, vol. 1216, no. 27, 2009, pp. 5165-70.
Saaid M, Saad B, Ali AS, et al. In situ derivatization hollow fibre liquid-phase microextraction for the determination of biogenic amines in food samples. J Chromatogr A. 2009;1216(27):5165-70.
Saaid, M., Saad, B., Ali, A. S., Saleh, M. I., Basheer, C., & Lee, H. K. (2009). In situ derivatization hollow fibre liquid-phase microextraction for the determination of biogenic amines in food samples. Journal of Chromatography. A, 1216(27), 5165-70. https://doi.org/10.1016/j.chroma.2009.04.091
Saaid M, et al. In Situ Derivatization Hollow Fibre Liquid-phase Microextraction for the Determination of Biogenic Amines in Food Samples. J Chromatogr A. 2009 Jul 3;1216(27):5165-70. PubMed PMID: 19481215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ derivatization hollow fibre liquid-phase microextraction for the determination of biogenic amines in food samples. AU - Saaid,Mardiana, AU - Saad,Bahruddin, AU - Ali,Abdussalam Salhin Mohamed, AU - Saleh,Muhammad Idiris, AU - Basheer,Chanbasha, AU - Lee,Hian Kee, Y1 - 2009/05/03/ PY - 2009/03/02/received PY - 2009/04/16/revised PY - 2009/04/24/accepted PY - 2009/6/2/entrez PY - 2009/6/2/pubmed PY - 2009/8/7/medline SP - 5165 EP - 70 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 27 N2 - Hollow fibre liquid-phase microextraction with in situ derivatization using dansyl chloride has been successfully developed for the high-performance liquid chromatography-ultraviolet (HPLC-UV) determination of the biogenic amines (tryptamine, putrescine, cadaverine, histamine, tyramine, spermidine) in food samples. Parameters affecting the performance of the in situ derivatization process such as type of extraction solvent, temperature, extraction time, stirring speed and salt addition were studied and optimized. Under the optimized conditions (extraction solvent, dihexyl ether; acceptor phase, 0.1M HCl; extraction time, 30 min; extraction temperature, 26 degrees C; without addition of salt), enrichment factors varying from 47 to 456 were achieved. Good linearity of the analytes was obtained over a concentration range of 0.1-5 microg mL(-1) (with correlation coefficients of 0.9901-0.9974). The limits of detection and quantification based on a signal-to-noise ratio of 3-10, ranged from 0.0075 to 0.030 microg mL(-1) and 0.03 to 0.10 microg mL(-1), respectively. The relative standard deviations based on the peak areas for six replicate analysis of water spiked with 0.5 microg mL(-1) of each biogenic amine were lower than 7.5%. The method was successfully applied to shrimp sauce and tomato ketchup samples, offering an interesting alternative to liquid-liquid extraction and solid phase extraction for the analysis of biogenic amines in food samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19481215/In_situ_derivatization_hollow_fibre_liquid_phase_microextraction_for_the_determination_of_biogenic_amines_in_food_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00667-0 DB - PRIME DP - Unbound Medicine ER -