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[Identification of Enterocytozoon bieneusi by PCR in stools of Tunisian immunocompromised patients].
Pathol Biol (Paris). 2011 Aug; 59(4):234-9.PB

Abstract

INTRODUCTION

Intestinal microsporidiosis is recognised as an important cause of opportunistic parasitosis in immunocompromised patients, especially HIV-infected patients. Enterocytozoon bieneusi is the common causal agent. The diagnosis of intestinal microsporidiosis has usually based on microscopic detection of the spores of microsporidia species in stool samples, requires additional staining techniques as Modified Weber's trichrome stain. However, the detection of the spores can be difficult and species determination, which is important for defining the appropriate treatment, is impossible. Polymerase chain reaction (PCR)-based methods have been successfully used for detection of microsporidian infections. They are more sensitive and are able to identify microsporidia species. The purpose of this study is to identify E. bieneusi to adapt treatment and assess the true prevalence of the intestinal microsporidiosis due to this species in compromised patients in Tunisia.

PATIENTS AND METHODS

One hundred and eighteen stools from immunocompromised patients, with a symptomatology in favour of the intestinal microsporidiosis, were analysed using light microscopy after staining with Modified Weber's trichrome stain and PCR.

RESULTS

Only four were positive by Modified Weber's trichrome stain whereas eleven stools were positive by PCR, giving a prevalence of 20% in HIV-infected patients and 5,35% in human immunodeficiency virus-negative patients.

CONCLUSION

This study confirms the usefulness of PCR in the diagnosis of the intestinal microsporidiosis due to E. bieneusi. Indeed, PCR has greater sensitivity than Modified Weber's trichrome stain and can identify the species of microsporidia in order to adapt the treatment.

Authors+Show Affiliations

Service de Parasitologie-Mycologie, CHU La Rabta, 15 Rue Djebel-Lakhdar, 1007 Tunis, Tunisie.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

fre

PubMed ID

19481368

Citation

Anane, S, et al. "[Identification of Enterocytozoon Bieneusi By PCR in Stools of Tunisian Immunocompromised Patients]." Pathologie-biologie, vol. 59, no. 4, 2011, pp. 234-9.
Anane S, Kaouech E, Belhadj S, et al. [Identification of Enterocytozoon bieneusi by PCR in stools of Tunisian immunocompromised patients]. Pathol Biol (Paris). 2011;59(4):234-9.
Anane, S., Kaouech, E., Belhadj, S., Abdelmalek, R., Ammari, L., Ben Othman, T., Bejaoui, M., Ben Chaabane, T., Kallel, K., & Chaker, E. (2011). [Identification of Enterocytozoon bieneusi by PCR in stools of Tunisian immunocompromised patients]. Pathologie-biologie, 59(4), 234-9. https://doi.org/10.1016/j.patbio.2009.03.007
Anane S, et al. [Identification of Enterocytozoon Bieneusi By PCR in Stools of Tunisian Immunocompromised Patients]. Pathol Biol (Paris). 2011;59(4):234-9. PubMed PMID: 19481368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Identification of Enterocytozoon bieneusi by PCR in stools of Tunisian immunocompromised patients]. AU - Anane,S, AU - Kaouech,E, AU - Belhadj,S, AU - Abdelmalek,R, AU - Ammari,L, AU - Ben Othman,T, AU - Bejaoui,M, AU - Ben Chaabane,T, AU - Kallel,K, AU - Chaker,E, Y1 - 2009/05/28/ PY - 2009/01/31/received PY - 2009/03/18/accepted PY - 2009/6/2/entrez PY - 2009/6/2/pubmed PY - 2011/12/31/medline SP - 234 EP - 9 JF - Pathologie-biologie JO - Pathol Biol (Paris) VL - 59 IS - 4 N2 - INTRODUCTION: Intestinal microsporidiosis is recognised as an important cause of opportunistic parasitosis in immunocompromised patients, especially HIV-infected patients. Enterocytozoon bieneusi is the common causal agent. The diagnosis of intestinal microsporidiosis has usually based on microscopic detection of the spores of microsporidia species in stool samples, requires additional staining techniques as Modified Weber's trichrome stain. However, the detection of the spores can be difficult and species determination, which is important for defining the appropriate treatment, is impossible. Polymerase chain reaction (PCR)-based methods have been successfully used for detection of microsporidian infections. They are more sensitive and are able to identify microsporidia species. The purpose of this study is to identify E. bieneusi to adapt treatment and assess the true prevalence of the intestinal microsporidiosis due to this species in compromised patients in Tunisia. PATIENTS AND METHODS: One hundred and eighteen stools from immunocompromised patients, with a symptomatology in favour of the intestinal microsporidiosis, were analysed using light microscopy after staining with Modified Weber's trichrome stain and PCR. RESULTS: Only four were positive by Modified Weber's trichrome stain whereas eleven stools were positive by PCR, giving a prevalence of 20% in HIV-infected patients and 5,35% in human immunodeficiency virus-negative patients. CONCLUSION: This study confirms the usefulness of PCR in the diagnosis of the intestinal microsporidiosis due to E. bieneusi. Indeed, PCR has greater sensitivity than Modified Weber's trichrome stain and can identify the species of microsporidia in order to adapt the treatment. SN - 1768-3114 UR - https://www.unboundmedicine.com/medline/citation/19481368/[Identification_of_Enterocytozoon_bieneusi_by_PCR_in_stools_of_Tunisian_immunocompromised_patients]_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0369-8114(09)00045-5 DB - PRIME DP - Unbound Medicine ER -