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A multi-biomarker assessment of the impact of the antibacterial trimethoprim on the non-target organism Zebra mussel (Dreissena polymorpha).
Comp Biochem Physiol C Toxicol Pharmacol. 2009 Sep; 150(3):329-36.CB

Abstract

A battery of eight biomarkers was applied in the freshwater mussel Dreissena polymorpha to evaluate potential sub-lethal effects of the antimicrobial trimethoprim (TMP, 5-[3,4,5-trimethoxybenzyl]pyrimidine-2,4-diamine). Mussels were exposed for 96 h to increasing concentrations (1, 3, 10 nM) of TMP in in vivo experiments. We determined the single cell gel electrophoresis (SCGE) assay, the micronucleus test (MN test), the apoptotic frequency (Halo assay) and the lysosomal membrane stability (Neutral Red Retention Assay) in mussel hemocytes. Moreover, to reveal whether the oxidative status was altered, measurements of the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the phase II detoxifying enzyme glutathione S-transferase (GST) were performed using the cytosolic fraction extracted from a pool of entire mussels. The biomarker battery pointed out only a moderate cyto- and genotoxicity on Zebra mussel hemocytes since only a slight increase in DNA damage was registered by apoptosis induction and MN frequency, while significant differences of lysosomal membrane stability from baseline levels were measured at 3 and 10 nM at the end of exposures only. Finally, TMP seems to have a very low induction capability or even an inhibitory effect on the activities of antioxidant enzymes, but a clear significant induction on GST.

Authors+Show Affiliations

University of Milan, Milan, Italy. andrea.binelli@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19481616

Citation

Binelli, A, et al. "A Multi-biomarker Assessment of the Impact of the Antibacterial Trimethoprim On the Non-target Organism Zebra Mussel (Dreissena Polymorpha)." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 150, no. 3, 2009, pp. 329-36.
Binelli A, Parolini M, Cogni D, et al. A multi-biomarker assessment of the impact of the antibacterial trimethoprim on the non-target organism Zebra mussel (Dreissena polymorpha). Comp Biochem Physiol C Toxicol Pharmacol. 2009;150(3):329-36.
Binelli, A., Parolini, M., Cogni, D., Pedriali, A., & Provini, A. (2009). A multi-biomarker assessment of the impact of the antibacterial trimethoprim on the non-target organism Zebra mussel (Dreissena polymorpha). Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 150(3), 329-36. https://doi.org/10.1016/j.cbpc.2009.05.011
Binelli A, et al. A Multi-biomarker Assessment of the Impact of the Antibacterial Trimethoprim On the Non-target Organism Zebra Mussel (Dreissena Polymorpha). Comp Biochem Physiol C Toxicol Pharmacol. 2009;150(3):329-36. PubMed PMID: 19481616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A multi-biomarker assessment of the impact of the antibacterial trimethoprim on the non-target organism Zebra mussel (Dreissena polymorpha). AU - Binelli,A, AU - Parolini,M, AU - Cogni,D, AU - Pedriali,A, AU - Provini,A, Y1 - 2009/05/27/ PY - 2009/04/27/received PY - 2009/05/14/revised PY - 2009/05/20/accepted PY - 2009/6/2/entrez PY - 2009/6/2/pubmed PY - 2009/10/29/medline SP - 329 EP - 36 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 150 IS - 3 N2 - A battery of eight biomarkers was applied in the freshwater mussel Dreissena polymorpha to evaluate potential sub-lethal effects of the antimicrobial trimethoprim (TMP, 5-[3,4,5-trimethoxybenzyl]pyrimidine-2,4-diamine). Mussels were exposed for 96 h to increasing concentrations (1, 3, 10 nM) of TMP in in vivo experiments. We determined the single cell gel electrophoresis (SCGE) assay, the micronucleus test (MN test), the apoptotic frequency (Halo assay) and the lysosomal membrane stability (Neutral Red Retention Assay) in mussel hemocytes. Moreover, to reveal whether the oxidative status was altered, measurements of the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the phase II detoxifying enzyme glutathione S-transferase (GST) were performed using the cytosolic fraction extracted from a pool of entire mussels. The biomarker battery pointed out only a moderate cyto- and genotoxicity on Zebra mussel hemocytes since only a slight increase in DNA damage was registered by apoptosis induction and MN frequency, while significant differences of lysosomal membrane stability from baseline levels were measured at 3 and 10 nM at the end of exposures only. Finally, TMP seems to have a very low induction capability or even an inhibitory effect on the activities of antioxidant enzymes, but a clear significant induction on GST. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/19481616/A_multi_biomarker_assessment_of_the_impact_of_the_antibacterial_trimethoprim_on_the_non_target_organism_Zebra_mussel__Dreissena_polymorpha__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(09)00124-0 DB - PRIME DP - Unbound Medicine ER -