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Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital.
Pathol Biol (Paris). 2011 Jun; 59(3):151-6.PB

Abstract

The prevalence of plasmid-mediated quinolone resistance (PMQR) determinants (qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA) was investigated in a collection of 47 extended-spectrum β-lactamase (ESBL) producing enterobacterial isolates with reduced susceptibility to fluoroquinolones, recovered at Nantes University hospital, in 2006. qnr, aac(6')-Ib-cr, and qepA genes were screened by PCR, and positive results were subsequently confirmed by sequencing. The epidemiological relationship between positive isolates was studied by pulsed-field gel electrophoresis (PFGE). qnr-positive isolates were analyzed for antimicrobial susceptibility and presence of mutations in the quinolone resistance-determining region (QRDR) of gyrA and parC genes. ESBL genes were characterized by PCR and sequencing. Conjugation experiments were performed to determine whether the qnr-carrying plasmids were self-transferable. Two Klebsiella pneumoniae isolates (4.3%), not clonally related, harboured a qnrS1 gene, whereas no qnrA- or qnrB-positive isolate was detected. The aac(6')-Ib-cr gene was detected in 11 Escherichia coli and one K. pneumoniae isolates. None of the 47 isolates carried the qepA gene. ESBLs associated with QnrS1 were CTX-M-14 and CTX-M-15. The CTX-M-15 producing isolate was highly resistant to fluoroquinolones and harboured three mutations in the QRDR and two PMQR determinants (qnrS1 and aac(6')-Ib-cr). The CTX-M-14-producing isolate exhibited reduced susceptibility or resistance to fluoroquinolones without resistance to nalidixic acid. This strain harboured only a qnr gene on a single 170 kb transferable plasmid, without any mutation in the QRDR. In conclusion, our study showed that aac(6')-Ib-cr gene had occurred in multiclonal ESBL-producing enterobacterial isolates collected at Nantes University hospital in 2006, with a higher prevalence than qnr genes.

Authors+Show Affiliations

Service de bactériologie-hygiène hospitalière, CHU de Nantes, 9, quai Moncousu, 44093 Nantes cedex 01, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19481883

Citation

Crémet, L, et al. "Prevalence of Plasmid-mediated Quinolone Resistance Determinants in ESBL Enterobacteriaceae Clinical Isolates Over a 1-year Period in a French Hospital." Pathologie-biologie, vol. 59, no. 3, 2011, pp. 151-6.
Crémet L, Caroff N, Dauvergne S, et al. Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. Pathol Biol. 2011;59(3):151-6.
Crémet, L., Caroff, N., Dauvergne, S., Reynaud, A., Lepelletier, D., & Corvec, S. (2011). Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. Pathologie-biologie, 59(3), 151-6. https://doi.org/10.1016/j.patbio.2009.04.003
Crémet L, et al. Prevalence of Plasmid-mediated Quinolone Resistance Determinants in ESBL Enterobacteriaceae Clinical Isolates Over a 1-year Period in a French Hospital. Pathol Biol. 2011;59(3):151-6. PubMed PMID: 19481883.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. AU - Crémet,L, AU - Caroff,N, AU - Dauvergne,S, AU - Reynaud,A, AU - Lepelletier,D, AU - Corvec,S, Y1 - 2009/05/29/ PY - 2009/03/18/received PY - 2009/04/03/accepted PY - 2009/6/2/entrez PY - 2009/6/2/pubmed PY - 2011/10/22/medline SP - 151 EP - 6 JF - Pathologie-biologie JO - Pathol. Biol. VL - 59 IS - 3 N2 - The prevalence of plasmid-mediated quinolone resistance (PMQR) determinants (qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA) was investigated in a collection of 47 extended-spectrum β-lactamase (ESBL) producing enterobacterial isolates with reduced susceptibility to fluoroquinolones, recovered at Nantes University hospital, in 2006. qnr, aac(6')-Ib-cr, and qepA genes were screened by PCR, and positive results were subsequently confirmed by sequencing. The epidemiological relationship between positive isolates was studied by pulsed-field gel electrophoresis (PFGE). qnr-positive isolates were analyzed for antimicrobial susceptibility and presence of mutations in the quinolone resistance-determining region (QRDR) of gyrA and parC genes. ESBL genes were characterized by PCR and sequencing. Conjugation experiments were performed to determine whether the qnr-carrying plasmids were self-transferable. Two Klebsiella pneumoniae isolates (4.3%), not clonally related, harboured a qnrS1 gene, whereas no qnrA- or qnrB-positive isolate was detected. The aac(6')-Ib-cr gene was detected in 11 Escherichia coli and one K. pneumoniae isolates. None of the 47 isolates carried the qepA gene. ESBLs associated with QnrS1 were CTX-M-14 and CTX-M-15. The CTX-M-15 producing isolate was highly resistant to fluoroquinolones and harboured three mutations in the QRDR and two PMQR determinants (qnrS1 and aac(6')-Ib-cr). The CTX-M-14-producing isolate exhibited reduced susceptibility or resistance to fluoroquinolones without resistance to nalidixic acid. This strain harboured only a qnr gene on a single 170 kb transferable plasmid, without any mutation in the QRDR. In conclusion, our study showed that aac(6')-Ib-cr gene had occurred in multiclonal ESBL-producing enterobacterial isolates collected at Nantes University hospital in 2006, with a higher prevalence than qnr genes. SN - 1768-3114 UR - https://www.unboundmedicine.com/medline/citation/19481883/Prevalence_of_plasmid_mediated_quinolone_resistance_determinants_in_ESBL_Enterobacteriaceae_clinical_isolates_over_a_1_year_period_in_a_French_hospital_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0369-8114(09)00049-2 DB - PRIME DP - Unbound Medicine ER -