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Transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 immunoreactive nerve fibers in human skin from individuals with and without Norrbottnian congenital insensitivity to pain.
Neuroscience. 2009 Sep 15; 162(4):1322-32.N

Abstract

Transient receptor potential vanilloid 1 (TRPV1), vanilloid 2 (TRPV2) and melastatin 8 (TRPM8) are thermosensitive cation channels expressed on primary sensory neurons. In contrast to TRPV1, which is present on nociceptive primary afferents and keratinocytes in human skin, less is known about the distribution of TRPV2 and TRPM8 in this tissue. Immunohistochemistry of human forearm skin identified TRPV2 and TRPM8 immunoreactive nerve fibers in epidermis-papillary dermis and around blood vessels and hair follicles in dermis, although these nerve fibers were less abundant than TRPV1 immunoreactive nerve fibers throughout the skin. The TRPV2 and TRPM8 immunoreactive nerve fibers also showed immunoreactivity for calcitonin gene-related peptide (CGRP) and to a lesser extent substance P (SP). Neither of the TRP ion channels co-localized with neurofilament 200 kDa (NF200), vasoactive intestinal peptide (VIP) or tyrosine hydroxylase (TH). Nerve fibers immunoreactive for TRPV1, TRPV2, TRPM8, CGRP and SP were absent or substantially reduced in number in individuals with Norrbottnian congenital insensitivity to pain, an autosomal disease selectively affecting the development of C-fiber and Adelta-fiber primary afferents. Quantitative real time PCR detected mRNA transcripts encoding TRPV1 and TRPV2, but not TRPM8, in skin from healthy volunteers, suggesting that these ion channels are also expressed extraneuronally. In conclusion, nerve fibers in human skin express TRPV1, TRPV2 and TRPM8 that co-localize with the sensory neuropeptides CGRP and SP, but not with NF200, VIP or TH. A dramatic loss of such nerve fibers was seen in skin from individuals with Norrbottnian congenital insensitivity to pain, further suggesting that these ion channels are expressed primarily on nociceptive primary sensory neurons in human skin.

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Authors+Show Affiliations

Axelsson HE
Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lund University Hospital, SE-221 85 Lund, Sweden.
Minde JK
No affiliation info available
Sonesson A
No affiliation info available
Toolanen G
No affiliation info available
Högestätt ED
No affiliation info available
Zygmunt PM
No affiliation info available

MeSH

AdolescentAdultAgedFemaleHumansMaleMiddle AgedNerve FibersPain Insensitivity, CongenitalSkinTRPM Cation ChannelsTRPV Cation ChannelsYoung Adult

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19482060

Citation

Axelsson, H E., et al. "Transient Receptor Potential Vanilloid 1, Vanilloid 2 and Melastatin 8 Immunoreactive Nerve Fibers in Human Skin From Individuals With and Without Norrbottnian Congenital Insensitivity to Pain." Neuroscience, vol. 162, no. 4, 2009, pp. 1322-32.
Axelsson HE, Minde JK, Sonesson A, et al. Transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 immunoreactive nerve fibers in human skin from individuals with and without Norrbottnian congenital insensitivity to pain. Neuroscience. 2009;162(4):1322-32.
Axelsson, H. E., Minde, J. K., Sonesson, A., Toolanen, G., Högestätt, E. D., & Zygmunt, P. M. (2009). Transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 immunoreactive nerve fibers in human skin from individuals with and without Norrbottnian congenital insensitivity to pain. Neuroscience, 162(4), 1322-32. https://doi.org/10.1016/j.neuroscience.2009.05.052
Axelsson HE, et al. Transient Receptor Potential Vanilloid 1, Vanilloid 2 and Melastatin 8 Immunoreactive Nerve Fibers in Human Skin From Individuals With and Without Norrbottnian Congenital Insensitivity to Pain. Neuroscience. 2009 Sep 15;162(4):1322-32. PubMed PMID: 19482060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 immunoreactive nerve fibers in human skin from individuals with and without Norrbottnian congenital insensitivity to pain. AU - Axelsson,H E, AU - Minde,J K, AU - Sonesson,A, AU - Toolanen,G, AU - Högestätt,E D, AU - Zygmunt,P M, Y1 - 2009/05/29/ PY - 2009/01/16/received PY - 2009/05/13/revised PY - 2009/05/15/accepted PY - 2009/6/2/entrez PY - 2009/6/2/pubmed PY - 2009/11/11/medline SP - 1322 EP - 32 JF - Neuroscience JO - Neuroscience VL - 162 IS - 4 N2 - Transient receptor potential vanilloid 1 (TRPV1), vanilloid 2 (TRPV2) and melastatin 8 (TRPM8) are thermosensitive cation channels expressed on primary sensory neurons. In contrast to TRPV1, which is present on nociceptive primary afferents and keratinocytes in human skin, less is known about the distribution of TRPV2 and TRPM8 in this tissue. Immunohistochemistry of human forearm skin identified TRPV2 and TRPM8 immunoreactive nerve fibers in epidermis-papillary dermis and around blood vessels and hair follicles in dermis, although these nerve fibers were less abundant than TRPV1 immunoreactive nerve fibers throughout the skin. The TRPV2 and TRPM8 immunoreactive nerve fibers also showed immunoreactivity for calcitonin gene-related peptide (CGRP) and to a lesser extent substance P (SP). Neither of the TRP ion channels co-localized with neurofilament 200 kDa (NF200), vasoactive intestinal peptide (VIP) or tyrosine hydroxylase (TH). Nerve fibers immunoreactive for TRPV1, TRPV2, TRPM8, CGRP and SP were absent or substantially reduced in number in individuals with Norrbottnian congenital insensitivity to pain, an autosomal disease selectively affecting the development of C-fiber and Adelta-fiber primary afferents. Quantitative real time PCR detected mRNA transcripts encoding TRPV1 and TRPV2, but not TRPM8, in skin from healthy volunteers, suggesting that these ion channels are also expressed extraneuronally. In conclusion, nerve fibers in human skin express TRPV1, TRPV2 and TRPM8 that co-localize with the sensory neuropeptides CGRP and SP, but not with NF200, VIP or TH. A dramatic loss of such nerve fibers was seen in skin from individuals with Norrbottnian congenital insensitivity to pain, further suggesting that these ion channels are expressed primarily on nociceptive primary sensory neurons in human skin. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/19482060/Transient_receptor_potential_vanilloid_1_vanilloid_2_and_melastatin_8_immunoreactive_nerve_fibers_in_human_skin_from_individuals_with_and_without_Norrbottnian_congenital_insensitivity_to_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(09)00847-1 DB - PRIME DP - Unbound Medicine ER -