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Relative changes in the abundance of branchial Na(+)/K(+)-ATPase alpha-isoform-like proteins in marine euryhaline milkfish (Chanos chanos) acclimated to environments of different salinities.
J Exp Zool A Ecol Genet Physiol. 2009 Aug 01; 311(7):521-9.JE

Abstract

Previous studies revealed that upon salinity challenge, milkfish (Chanos chanos), the euryhaline teleost, exhibited adaptive changes in branchial Na(+)/K(+)-ATPase (NKA) activity with different Na(+) and K(+) affinities. Since alteration of activity and ion-affinity may be influenced by changes in different isoforms of NKA alpha-subunit (i.e., the catalytic subunit), it is, thus, intriguing to compare the patterns of protein abundance of three major NKA alpha-isoform-like proteins (i.e., alpha1, alpha2, and alpha3) in the gills of euryhaline milkfish following salinity challenge. The protein abundance of three NKA alpha-isoform-like proteins in gills of milkfish reared in seawater (SW), fresh water (FW), as well as hypersaline water (HSW, 60 per thousand) were analyzed by immunoblotting. In the acclimation experiments, the SW group revealed significantly higher levels of NKA alpha1- and alpha3-like proteins than the FW or HSW group. Time-course experiments on milkfish that were transferred from SW to HSW revealed the abundance of branchial NKA alpha1-like and alpha3-like proteins decreased significantly after 96 and 12 hr, respectively, and no significant difference was found in NKA alpha2-like protein. Furthermore, when fish were transferred from SW to FW, the amounts of NKA alpha1- and alpha3-like proteins was significantly decreased after 96 hr. Taken together, acute and chronic changes in the abundance of branchial NKA alpha1- and alpha3-like proteins may fulfill the requirements of altering NKA activity with different Na(+) or K(+) affinity for euryhaline milkfish acclimated to environments of various salinities.

Authors+Show Affiliations

Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19484743

Citation

Tang, Cheng-Hao, et al. "Relative Changes in the Abundance of Branchial Na(+)/K(+)-ATPase Alpha-isoform-like Proteins in Marine Euryhaline Milkfish (Chanos Chanos) Acclimated to Environments of Different Salinities." Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology, vol. 311, no. 7, 2009, pp. 521-9.
Tang CH, Chiu YH, Tsai SC, et al. Relative changes in the abundance of branchial Na(+)/K(+)-ATPase alpha-isoform-like proteins in marine euryhaline milkfish (Chanos chanos) acclimated to environments of different salinities. J Exp Zool A Ecol Genet Physiol. 2009;311(7):521-9.
Tang, C. H., Chiu, Y. H., Tsai, S. C., & Lee, T. H. (2009). Relative changes in the abundance of branchial Na(+)/K(+)-ATPase alpha-isoform-like proteins in marine euryhaline milkfish (Chanos chanos) acclimated to environments of different salinities. Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology, 311(7), 521-9. https://doi.org/10.1002/jez.547
Tang CH, et al. Relative Changes in the Abundance of Branchial Na(+)/K(+)-ATPase Alpha-isoform-like Proteins in Marine Euryhaline Milkfish (Chanos Chanos) Acclimated to Environments of Different Salinities. J Exp Zool A Ecol Genet Physiol. 2009 Aug 1;311(7):521-9. PubMed PMID: 19484743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relative changes in the abundance of branchial Na(+)/K(+)-ATPase alpha-isoform-like proteins in marine euryhaline milkfish (Chanos chanos) acclimated to environments of different salinities. AU - Tang,Cheng-Hao, AU - Chiu,Yu-Huei, AU - Tsai,Shu-Chuan, AU - Lee,Tsung-Han, PY - 2009/6/2/entrez PY - 2009/6/2/pubmed PY - 2009/9/18/medline SP - 521 EP - 9 JF - Journal of experimental zoology. Part A, Ecological genetics and physiology JO - J Exp Zool A Ecol Genet Physiol VL - 311 IS - 7 N2 - Previous studies revealed that upon salinity challenge, milkfish (Chanos chanos), the euryhaline teleost, exhibited adaptive changes in branchial Na(+)/K(+)-ATPase (NKA) activity with different Na(+) and K(+) affinities. Since alteration of activity and ion-affinity may be influenced by changes in different isoforms of NKA alpha-subunit (i.e., the catalytic subunit), it is, thus, intriguing to compare the patterns of protein abundance of three major NKA alpha-isoform-like proteins (i.e., alpha1, alpha2, and alpha3) in the gills of euryhaline milkfish following salinity challenge. The protein abundance of three NKA alpha-isoform-like proteins in gills of milkfish reared in seawater (SW), fresh water (FW), as well as hypersaline water (HSW, 60 per thousand) were analyzed by immunoblotting. In the acclimation experiments, the SW group revealed significantly higher levels of NKA alpha1- and alpha3-like proteins than the FW or HSW group. Time-course experiments on milkfish that were transferred from SW to HSW revealed the abundance of branchial NKA alpha1-like and alpha3-like proteins decreased significantly after 96 and 12 hr, respectively, and no significant difference was found in NKA alpha2-like protein. Furthermore, when fish were transferred from SW to FW, the amounts of NKA alpha1- and alpha3-like proteins was significantly decreased after 96 hr. Taken together, acute and chronic changes in the abundance of branchial NKA alpha1- and alpha3-like proteins may fulfill the requirements of altering NKA activity with different Na(+) or K(+) affinity for euryhaline milkfish acclimated to environments of various salinities. SN - 1932-5223 UR - https://www.unboundmedicine.com/medline/citation/19484743/Relative_changes_in_the_abundance_of_branchial_Na_+_/K_+__ATPase_alpha_isoform_like_proteins_in_marine_euryhaline_milkfish__Chanos_chanos__acclimated_to_environments_of_different_salinities_ L2 - https://doi.org/10.1002/jez.547 DB - PRIME DP - Unbound Medicine ER -