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Pulmonary periarterial inflammation in fatal asthma.
Clin Exp Allergy. 2009 Oct; 39(10):1499-507.CE

Abstract

BACKGROUND

To date, little information has been available about pulmonary artery pathology in asthma. The pulmonary artery supplies the distal parts of the lungs and likely represents a site of immunological reaction in allergic inflammation. The objective of this study was to describe the inflammatory cell phenotype of pulmonary artery adventitial inflammation in lung tissue from patients who died of asthma.

METHODS

We quantified the different inflammatory cell types in the periarterial region of small pulmonary arteries in lung tissue from 22 patients who died of asthma [fatal asthma (FA)] and 10 control subjects. Using immunohistochemistry and image analysis, we quantified the cell density for T lymphocytes (CD3, CD4, CD8), B lymphocytes (CD20), eosinophils, mast cells (chymase and tryptase), and neutrophils in the adventitial layer of pulmonary arteries with a diameter smaller than 500 microm.

RESULTS

Our data (median/interquartile range) demonstrated increased cell density of mast cells [FA=271.8 (148.7) cells/mm2; controls=177.0 (130.3) cells/mm2, P=0.026], eosinophils [FA=23.1 (58.6) cells/mm2; controls=0.0 (2.3) cells/mm2, P=0.012], and neutrophils [FA=50.4 (85.5) cells/mm2; controls=2.9 (30.5) cells/mm2, P=0.009] in the periarterial space in FA. No significant differences were found for B and T lymphocytes or CD4+ or CD8+ subsets. Chymase/tryptase positive (MCCT) mast cells predominated over tryptase (MCT) mast cells in the perivascular arterial space in both asthma patients and controls [MCCT/(MCCT+MCT)=0.91 (0-1) in FA and 0.75 (0-1) in controls, P=0.86].

CONCLUSIONS

Our results show that the adventitial layer of the pulmonary artery participates in the inflammatory process in FA, demonstrating increased infiltration of mast cells, eosinophils, and neutrophils, but not of T and B lymphocytes.

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Authors+Show Affiliations

Shiang C
Department of Pathology, School of Medicine, São Paulo University, São Paulo, SP, Brazil.
Mauad T
No affiliation info available
Senhorini A
No affiliation info available
de Araújo BB
No affiliation info available
Ferreira DS
No affiliation info available
da Silva LF
No affiliation info available
Dolhnikoff M
No affiliation info available
Tsokos M
No affiliation info available
Rabe KF
No affiliation info available
Pabst R
No affiliation info available

MeSH

AdolescentAdultAgedAntigens, CDArteritisAsthmaChildChymasesFemaleHumansInflammationLeukocytesLungMaleMast CellsMiddle AgedPulmonary ArteryRetrospective StudiesTryptases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19486035

Citation

Shiang, C, et al. "Pulmonary Periarterial Inflammation in Fatal Asthma." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 39, no. 10, 2009, pp. 1499-507.
Shiang C, Mauad T, Senhorini A, et al. Pulmonary periarterial inflammation in fatal asthma. Clin Exp Allergy. 2009;39(10):1499-507.
Shiang, C., Mauad, T., Senhorini, A., de Araújo, B. B., Ferreira, D. S., da Silva, L. F., Dolhnikoff, M., Tsokos, M., Rabe, K. F., & Pabst, R. (2009). Pulmonary periarterial inflammation in fatal asthma. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 39(10), 1499-507. https://doi.org/10.1111/j.1365-2222.2009.03281.x
Shiang C, et al. Pulmonary Periarterial Inflammation in Fatal Asthma. Clin Exp Allergy. 2009;39(10):1499-507. PubMed PMID: 19486035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pulmonary periarterial inflammation in fatal asthma. AU - Shiang,C, AU - Mauad,T, AU - Senhorini,A, AU - de Araújo,B B, AU - Ferreira,D S, AU - da Silva,L F F, AU - Dolhnikoff,M, AU - Tsokos,M, AU - Rabe,K F, AU - Pabst,R, Y1 - 2009/05/22/ PY - 2009/6/3/entrez PY - 2009/6/3/pubmed PY - 2010/2/18/medline SP - 1499 EP - 507 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 39 IS - 10 N2 - BACKGROUND: To date, little information has been available about pulmonary artery pathology in asthma. The pulmonary artery supplies the distal parts of the lungs and likely represents a site of immunological reaction in allergic inflammation. The objective of this study was to describe the inflammatory cell phenotype of pulmonary artery adventitial inflammation in lung tissue from patients who died of asthma. METHODS: We quantified the different inflammatory cell types in the periarterial region of small pulmonary arteries in lung tissue from 22 patients who died of asthma [fatal asthma (FA)] and 10 control subjects. Using immunohistochemistry and image analysis, we quantified the cell density for T lymphocytes (CD3, CD4, CD8), B lymphocytes (CD20), eosinophils, mast cells (chymase and tryptase), and neutrophils in the adventitial layer of pulmonary arteries with a diameter smaller than 500 microm. RESULTS: Our data (median/interquartile range) demonstrated increased cell density of mast cells [FA=271.8 (148.7) cells/mm2; controls=177.0 (130.3) cells/mm2, P=0.026], eosinophils [FA=23.1 (58.6) cells/mm2; controls=0.0 (2.3) cells/mm2, P=0.012], and neutrophils [FA=50.4 (85.5) cells/mm2; controls=2.9 (30.5) cells/mm2, P=0.009] in the periarterial space in FA. No significant differences were found for B and T lymphocytes or CD4+ or CD8+ subsets. Chymase/tryptase positive (MCCT) mast cells predominated over tryptase (MCT) mast cells in the perivascular arterial space in both asthma patients and controls [MCCT/(MCCT+MCT)=0.91 (0-1) in FA and 0.75 (0-1) in controls, P=0.86]. CONCLUSIONS: Our results show that the adventitial layer of the pulmonary artery participates in the inflammatory process in FA, demonstrating increased infiltration of mast cells, eosinophils, and neutrophils, but not of T and B lymphocytes. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/19486035/Pulmonary_periarterial_inflammation_in_fatal_asthma_ L2 - https://doi.org/10.1111/j.1365-2222.2009.03281.x DB - PRIME DP - Unbound Medicine ER -