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Water extracts of Brassica oleracea var. costata potentiate paraquat toxicity to rat hepatocytes in vitro.
Toxicol In Vitro. 2009 Sep; 23(6):1131-8.TV

Abstract

Tronchuda cabbage extracts have been proven to have antioxidant potential against various oxidative species in cell free systems, though its antioxidant potential in cellular models remained to be demonstrated. In the present study, we used primary cultures of rat hepatocytes for the cellular assay system and paraquat PQ exposure as a pro-oxidant model agent, to test whether tronchuda cabbage hydrolysed water extracts provide protective or aggravating effects towards PQ-induced oxidative stress and cell death. For this purpose cellular parameters related to oxidative stress were measured, namely the generation of superoxide anion, glutathione oxidation, lipid peroxidation, intracellular ATP levels, activation of nuclear factor-kappaB (NF-kappaB), activity of antioxidant enzymes, and cell death. The obtained results demonstrated that the studied hydrolysed water extracts of tronchuda cabbage, especially rich in kaempferol (84%) and other polyphenols, namely hydroxycinnamic acids and traces of quercetin, can potentiate the toxicity of PQ in primary cultures of rat hepatocytes. These results highlight that prospective antioxidant effects of plant extracts, observed in vitro, using non-cellular systems, are not always confirmed in cellular models, in which the concentrations required to scavenge pro-oxidant species may be highly detrimental to the cells.

Authors+Show Affiliations

REQUIMTE, Toxicology Department, Faculty of Pharmacy, University of Porto, 4099-030 Porto, Portugal. csousa@ff.up.ptNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19486935

Citation

Sousa, C, et al. "Water Extracts of Brassica Oleracea Var. Costata Potentiate Paraquat Toxicity to Rat Hepatocytes in Vitro." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 23, no. 6, 2009, pp. 1131-8.
Sousa C, Pontes H, Carmo H, et al. Water extracts of Brassica oleracea var. costata potentiate paraquat toxicity to rat hepatocytes in vitro. Toxicol In Vitro. 2009;23(6):1131-8.
Sousa, C., Pontes, H., Carmo, H., Dinis-Oliveira, R. J., Valentão, P., Andrade, P. B., Remião, F., Bastos, M. L., & Carvalho, F. (2009). Water extracts of Brassica oleracea var. costata potentiate paraquat toxicity to rat hepatocytes in vitro. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 23(6), 1131-8. https://doi.org/10.1016/j.tiv.2009.05.012
Sousa C, et al. Water Extracts of Brassica Oleracea Var. Costata Potentiate Paraquat Toxicity to Rat Hepatocytes in Vitro. Toxicol In Vitro. 2009;23(6):1131-8. PubMed PMID: 19486935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Water extracts of Brassica oleracea var. costata potentiate paraquat toxicity to rat hepatocytes in vitro. AU - Sousa,C, AU - Pontes,H, AU - Carmo,H, AU - Dinis-Oliveira,R J, AU - Valentão,P, AU - Andrade,P B, AU - Remião,F, AU - Bastos,M L, AU - Carvalho,F, Y1 - 2009/05/30/ PY - 2009/04/09/received PY - 2009/05/02/revised PY - 2009/05/26/accepted PY - 2009/6/3/entrez PY - 2009/6/3/pubmed PY - 2009/12/16/medline SP - 1131 EP - 8 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 23 IS - 6 N2 - Tronchuda cabbage extracts have been proven to have antioxidant potential against various oxidative species in cell free systems, though its antioxidant potential in cellular models remained to be demonstrated. In the present study, we used primary cultures of rat hepatocytes for the cellular assay system and paraquat PQ exposure as a pro-oxidant model agent, to test whether tronchuda cabbage hydrolysed water extracts provide protective or aggravating effects towards PQ-induced oxidative stress and cell death. For this purpose cellular parameters related to oxidative stress were measured, namely the generation of superoxide anion, glutathione oxidation, lipid peroxidation, intracellular ATP levels, activation of nuclear factor-kappaB (NF-kappaB), activity of antioxidant enzymes, and cell death. The obtained results demonstrated that the studied hydrolysed water extracts of tronchuda cabbage, especially rich in kaempferol (84%) and other polyphenols, namely hydroxycinnamic acids and traces of quercetin, can potentiate the toxicity of PQ in primary cultures of rat hepatocytes. These results highlight that prospective antioxidant effects of plant extracts, observed in vitro, using non-cellular systems, are not always confirmed in cellular models, in which the concentrations required to scavenge pro-oxidant species may be highly detrimental to the cells. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/19486935/Water_extracts_of_Brassica_oleracea_var__costata_potentiate_paraquat_toxicity_to_rat_hepatocytes_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(09)00116-7 DB - PRIME DP - Unbound Medicine ER -