Prime

Type your tag names separated by a space and hit enter

[Effect of electroacupuncture scalp point-through-point therapy on the expression of tyrosine hydroxylase and dopamine transporter mRNAs in substantia nigra of Parkinson's disease model rats].

Abstract

OBJECTIVE

To explore the mechanism of electroacupuncture scalp point-through-point therapy in the treatment of Parkinson's disease.

METHODS

Thirty-six Wistar rats were randomly divided into a normal group, a sham-operation group, a model group and a point-through-point therapy group. 6-()HDA was injected into left striatum to made lateralization Parkinson's disease rat model. The point-through-point therapy group was treated with electroacupuncture at "Baihui" (GV 20 )-through-"Taiyang" (EX-NH 5), once each day, 6 days constituting one course, for 2 courses, and the rats of other groups were not treated. HE staining method was used for observation of the histo-morphologic changes of the substantia nigra neurons, and RT-PCR for the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) mRNAs.

RESULTS

The expressions of TH mRNA (1.22 +/- 0. 19) and DAT mRNA (0.62-0.11) in the point-through-point therapy group were significantly higher than (0.65 +/- 0.17) and (0.41 +/- 0.08) in the model group, respectively (all P < 0.05). As compared with the model group, the number of neurons in the substantia nigra increased and degeneration of the neurons relieved in the point-through-point therapy group.

CONCLUSION

The electroacupuncture scalp point-through-point therapy can increase expressions of TH mRNA and DAT mRNA in the substantia nigra in the Parkinson's disease model rat, and promote synthesis and reuptake of dopamine, hence Parkinson's disease is cured.

Authors+Show Affiliations

,

Heilongjiang Academy of TCM, Harbin 150036, China. hljwang@yahoo.com.cn

,

Source

MeSH

Acupuncture Points
Animals
Disease Models, Animal
Dopamine Plasma Membrane Transport Proteins
Electroacupuncture
Female
Gene Expression
Humans
Male
Parkinson Disease
RNA, Messenger
Random Allocation
Rats
Rats, Wistar
Substantia Nigra
Tyrosine 3-Monooxygenase

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19489498

Citation

TY - JOUR T1 - [Effect of electroacupuncture scalp point-through-point therapy on the expression of tyrosine hydroxylase and dopamine transporter mRNAs in substantia nigra of Parkinson's disease model rats]. AU - Wang,Shun, AU - Qi,Xiu-jie, AU - Han,Di, PY - 2009/6/4/entrez PY - 2009/6/6/pubmed PY - 2009/6/25/medline SP - 391 EP - 4 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 29 IS - 5 N2 - OBJECTIVE: To explore the mechanism of electroacupuncture scalp point-through-point therapy in the treatment of Parkinson's disease. METHODS: Thirty-six Wistar rats were randomly divided into a normal group, a sham-operation group, a model group and a point-through-point therapy group. 6-()HDA was injected into left striatum to made lateralization Parkinson's disease rat model. The point-through-point therapy group was treated with electroacupuncture at "Baihui" (GV 20 )-through-"Taiyang" (EX-NH 5), once each day, 6 days constituting one course, for 2 courses, and the rats of other groups were not treated. HE staining method was used for observation of the histo-morphologic changes of the substantia nigra neurons, and RT-PCR for the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) mRNAs. RESULTS: The expressions of TH mRNA (1.22 +/- 0. 19) and DAT mRNA (0.62-0.11) in the point-through-point therapy group were significantly higher than (0.65 +/- 0.17) and (0.41 +/- 0.08) in the model group, respectively (all P < 0.05). As compared with the model group, the number of neurons in the substantia nigra increased and degeneration of the neurons relieved in the point-through-point therapy group. CONCLUSION: The electroacupuncture scalp point-through-point therapy can increase expressions of TH mRNA and DAT mRNA in the substantia nigra in the Parkinson's disease model rat, and promote synthesis and reuptake of dopamine, hence Parkinson's disease is cured. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/19489498/ L2 - https://medlineplus.gov/parkinsonsdisease.html ER -