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Elevated concentrations of liver-expressed chemokine/CC chemokine ligand 16 in bronchoalveolar lavage fluid from patients with eosinophilic pneumonia.
Int Arch Allergy Immunol. 2009; 150(3):282-90.IA

Abstract

BACKGROUND

Eosinophilic pneumonia is characterized by the prominent accumulation of eosinophils and lymphocytes in the lung parenchyma. Liver-expressed chemokine (LEC)/CC chemokine ligand 16 (CCL16) is a novel functional ligand for H4 which is expressed on eosinophils and also an affinity ligand for CCR1, CCR2, CCR5 and CCR8 which are expressed on T lymphocytes and monocytes. The purpose of this study is to clarify the role of LEC/CCL16 in eosinophilic pneumonia.

METHODS

The LEC/CCL16 level was measured using an enzyme-linked immunosorbent assay in the bronchoalveolar lavage fluid (BALF) of 33 patients with eosinophilic pneumonia, 26 patients with sarcoidosis and 10 healthy volunteers. The cell sources of LEC/CCL16 in BALF were evaluated by immunocytochemistry.

RESULTS

The LEC/CCL16 levels in BALF from patients with eosinophilic pneumonia were significantly higher than those from patients with sarcoidosis and healthy volunteers. The BALF LEC/CCL16 levels correlated with the numbers of BALF eosinophils and lymphocytes, respectively. The BALF LEC/CCL16 levels were significantly decreased after remission in eosinophilic pneumonia. In immunocytochemistry, the LEC/CCL16 expression was clearly observed in CD1a-positive dendritic cells as well as in CD68-positive macrophages harvested from patients with eosinophilic pneumonia, but not from the controls.

CONCLUSIONS

These results suggest that LEC/CCL16 produced by dendritic cells as well as by alveolar macrophages contributes to the accumulation of eosinophils and lymphocytes into the inflamed lungs of patients with eosinophilic pneumonia.

Authors+Show Affiliations

Department of Internal Medicine 3, Faculty of Medicine, Oita University, Oita, Japan. nureki@med.oita-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19494526

Citation

Nureki, Shin-ichi, et al. "Elevated Concentrations of Liver-expressed chemokine/CC Chemokine Ligand 16 in Bronchoalveolar Lavage Fluid From Patients With Eosinophilic Pneumonia." International Archives of Allergy and Immunology, vol. 150, no. 3, 2009, pp. 282-90.
Nureki S, Miyazaki E, Usagawa Y, et al. Elevated concentrations of liver-expressed chemokine/CC chemokine ligand 16 in bronchoalveolar lavage fluid from patients with eosinophilic pneumonia. Int Arch Allergy Immunol. 2009;150(3):282-90.
Nureki, S., Miyazaki, E., Usagawa, Y., Ueno, T., Ando, M., Takenaka, R., Ito, T., Ishii, T., & Kumamoto, T. (2009). Elevated concentrations of liver-expressed chemokine/CC chemokine ligand 16 in bronchoalveolar lavage fluid from patients with eosinophilic pneumonia. International Archives of Allergy and Immunology, 150(3), 282-90. https://doi.org/10.1159/000222681
Nureki S, et al. Elevated Concentrations of Liver-expressed chemokine/CC Chemokine Ligand 16 in Bronchoalveolar Lavage Fluid From Patients With Eosinophilic Pneumonia. Int Arch Allergy Immunol. 2009;150(3):282-90. PubMed PMID: 19494526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated concentrations of liver-expressed chemokine/CC chemokine ligand 16 in bronchoalveolar lavage fluid from patients with eosinophilic pneumonia. AU - Nureki,Shin-ichi, AU - Miyazaki,Eishi, AU - Usagawa,Yuko, AU - Ueno,Takuya, AU - Ando,Masaru, AU - Takenaka,Ryuichi, AU - Ito,Takeo, AU - Ishii,Toshihiro, AU - Kumamoto,Toshihide, Y1 - 2009/06/04/ PY - 2008/10/15/received PY - 2009/02/16/accepted PY - 2009/6/5/entrez PY - 2009/6/6/pubmed PY - 2009/12/30/medline SP - 282 EP - 90 JF - International archives of allergy and immunology JO - Int Arch Allergy Immunol VL - 150 IS - 3 N2 - BACKGROUND: Eosinophilic pneumonia is characterized by the prominent accumulation of eosinophils and lymphocytes in the lung parenchyma. Liver-expressed chemokine (LEC)/CC chemokine ligand 16 (CCL16) is a novel functional ligand for H4 which is expressed on eosinophils and also an affinity ligand for CCR1, CCR2, CCR5 and CCR8 which are expressed on T lymphocytes and monocytes. The purpose of this study is to clarify the role of LEC/CCL16 in eosinophilic pneumonia. METHODS: The LEC/CCL16 level was measured using an enzyme-linked immunosorbent assay in the bronchoalveolar lavage fluid (BALF) of 33 patients with eosinophilic pneumonia, 26 patients with sarcoidosis and 10 healthy volunteers. The cell sources of LEC/CCL16 in BALF were evaluated by immunocytochemistry. RESULTS: The LEC/CCL16 levels in BALF from patients with eosinophilic pneumonia were significantly higher than those from patients with sarcoidosis and healthy volunteers. The BALF LEC/CCL16 levels correlated with the numbers of BALF eosinophils and lymphocytes, respectively. The BALF LEC/CCL16 levels were significantly decreased after remission in eosinophilic pneumonia. In immunocytochemistry, the LEC/CCL16 expression was clearly observed in CD1a-positive dendritic cells as well as in CD68-positive macrophages harvested from patients with eosinophilic pneumonia, but not from the controls. CONCLUSIONS: These results suggest that LEC/CCL16 produced by dendritic cells as well as by alveolar macrophages contributes to the accumulation of eosinophils and lymphocytes into the inflamed lungs of patients with eosinophilic pneumonia. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/19494526/Elevated_concentrations_of_liver_expressed_chemokine/CC_chemokine_ligand_16_in_bronchoalveolar_lavage_fluid_from_patients_with_eosinophilic_pneumonia_ L2 - https://www.karger.com?DOI=10.1159/000222681 DB - PRIME DP - Unbound Medicine ER -