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Antinociceptive and anti-inflammatory effects of Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae) in experimental animal models.
Med Princ Pract. 2009; 18(4):272-9.MP

Abstract

OBJECTIVE

The present study was carried out to explore the antinociceptive as well as the anti-inflammatory effects of an ethanol extract of Stachytarpheta jamaicensis (L.) Vahl (EESJ) using 3 models of nociception and 2 models of inflammation in experimental animals.

MATERIALS AND METHODS

EESJ was prepared by overnight soaking of the oven-dried (50 degrees C; 72 h) ground leaves (500 g) in 80% ethanol (1:5; w/v). The filtrate was evaporated to dryness (50 degrees C), resuspended in distilled water at concentrations to provide the desired doses of 50, 100 and 150 mg/kg. For antinociceptive effects, 3 models were used: acetic acid-induced abdominal writhing, hot-plate- and formalin-induced paw-licking tests; for anti-inflammatory effects, 2 models were used--carrageenan-induced paw edema and cotton-pellet-induced granuloma tests. Appropriate doses were administered intraperitoneally (i.p.) to mice/rats prior to each test. The mechanisms of antinociceptive action of the extract were also investigated by pretreatment with naloxone (5 mg/kg, i.p.).

RESULTS

The extract exhibited significant (p < 0.05) antinociceptive activity in all nociceptive models tested with dose-dependent activity observed using the abdominal writhing and formalin tests. Pretreatment with naloxone partially, but significantly (p < 0.05) reversed the antinociceptive activity of the extract when assessed using the abdominal-writhing- and formalin-induced paw-licking tests, and completely inhibited its activity when the hot-plate test was used. The extract also showed significant (p < 0.05) anti-inflammatory activity in both the acute (carrageenan-induced paw edema test) and the chronic (cotton-pellet granuloma test) tests.

CONCLUSION

This study showed the potential of EESJ to exert antinociceptive and anti-inflammatory activities, the former being modulated via peripheral and central mechanisms and involving, in part, activation of the opioid receptor system.

Authors+Show Affiliations

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia. shaza8174@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19494533

Citation

Sulaiman, M R., et al. "Antinociceptive and Anti-inflammatory Effects of Stachytarpheta Jamaicensis (L.) Vahl (Verbenaceae) in Experimental Animal Models." Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, vol. 18, no. 4, 2009, pp. 272-9.
Sulaiman MR, Zakaria ZA, Chiong HS, et al. Antinociceptive and anti-inflammatory effects of Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae) in experimental animal models. Med Princ Pract. 2009;18(4):272-9.
Sulaiman, M. R., Zakaria, Z. A., Chiong, H. S., Lai, S. K., Israf, D. A., & Azam Shah, T. M. (2009). Antinociceptive and anti-inflammatory effects of Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae) in experimental animal models. Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, 18(4), 272-9. https://doi.org/10.1159/000215723
Sulaiman MR, et al. Antinociceptive and Anti-inflammatory Effects of Stachytarpheta Jamaicensis (L.) Vahl (Verbenaceae) in Experimental Animal Models. Med Princ Pract. 2009;18(4):272-9. PubMed PMID: 19494533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antinociceptive and anti-inflammatory effects of Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae) in experimental animal models. AU - Sulaiman,M R, AU - Zakaria,Z A, AU - Chiong,H S, AU - Lai,S K, AU - Israf,D A, AU - Azam Shah,T M Tg, Y1 - 2009/06/02/ PY - 2007/10/02/received PY - 2007/11/25/accepted PY - 2009/6/5/entrez PY - 2009/6/6/pubmed PY - 2009/8/19/medline SP - 272 EP - 9 JF - Medical principles and practice : international journal of the Kuwait University, Health Science Centre JO - Med Princ Pract VL - 18 IS - 4 N2 - OBJECTIVE: The present study was carried out to explore the antinociceptive as well as the anti-inflammatory effects of an ethanol extract of Stachytarpheta jamaicensis (L.) Vahl (EESJ) using 3 models of nociception and 2 models of inflammation in experimental animals. MATERIALS AND METHODS: EESJ was prepared by overnight soaking of the oven-dried (50 degrees C; 72 h) ground leaves (500 g) in 80% ethanol (1:5; w/v). The filtrate was evaporated to dryness (50 degrees C), resuspended in distilled water at concentrations to provide the desired doses of 50, 100 and 150 mg/kg. For antinociceptive effects, 3 models were used: acetic acid-induced abdominal writhing, hot-plate- and formalin-induced paw-licking tests; for anti-inflammatory effects, 2 models were used--carrageenan-induced paw edema and cotton-pellet-induced granuloma tests. Appropriate doses were administered intraperitoneally (i.p.) to mice/rats prior to each test. The mechanisms of antinociceptive action of the extract were also investigated by pretreatment with naloxone (5 mg/kg, i.p.). RESULTS: The extract exhibited significant (p < 0.05) antinociceptive activity in all nociceptive models tested with dose-dependent activity observed using the abdominal writhing and formalin tests. Pretreatment with naloxone partially, but significantly (p < 0.05) reversed the antinociceptive activity of the extract when assessed using the abdominal-writhing- and formalin-induced paw-licking tests, and completely inhibited its activity when the hot-plate test was used. The extract also showed significant (p < 0.05) anti-inflammatory activity in both the acute (carrageenan-induced paw edema test) and the chronic (cotton-pellet granuloma test) tests. CONCLUSION: This study showed the potential of EESJ to exert antinociceptive and anti-inflammatory activities, the former being modulated via peripheral and central mechanisms and involving, in part, activation of the opioid receptor system. SN - 1423-0151 UR - https://www.unboundmedicine.com/medline/citation/19494533/Antinociceptive_and_anti_inflammatory_effects_of_Stachytarpheta_jamaicensis__L___Vahl__Verbenaceae__in_experimental_animal_models_ L2 - https://www.karger.com?DOI=10.1159/000215723 DB - PRIME DP - Unbound Medicine ER -