Tags

Type your tag names separated by a space and hit enter

High genetic diversity of Sappinia-like strains (Amoebozoa, Thecamoebidae) revealed by SSU rRNA investigations.
Parasitol Res. 2009 Sep; 105(3):869-73.PR

Abstract

Sappinia diploidea is known as a free-living amoeba of worldwide distribution and has also been reported as causative agent of a brain infection in an immunocompetent young man. In the current study, we were able to isolate eight strains of S. diploidea-like amoebae identified by light microscopy from different habitats. Cultures of all strains were established successfully for molecular characterization. The small subunit ribosomal RNA genes of all strains were sequenced and compared to one another, to the neotype of S. diploidea, and to strains of Sappinia pedata, the only other Sappinia species known to date, from GenBank by multiple sequence alignment and cluster analysis. Altogether, the phylogenetic position of the genus Sappinia within the Thecamoebidae was corroborated; however, it was shown that the genus splits into several well-separated clusters making the establishment of new species within this genus inevitable. Furthermore, two of the S. diploidea-like strains were actually more closely related to S. pedata than to S. diploidea, although the diagnostically relevant standing form which seems to be characteristic for S. pedata was not observed in either of the two strains.

Authors+Show Affiliations

Department of General Ecology, Zoological Institute, University of Cologne, Cologne, Germany. claudia.wylezich@io-warnemuende.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19495795

Citation

Wylezich, Claudia, et al. "High Genetic Diversity of Sappinia-like Strains (Amoebozoa, Thecamoebidae) Revealed By SSU rRNA Investigations." Parasitology Research, vol. 105, no. 3, 2009, pp. 869-73.
Wylezich C, Walochnik J, Michel R. High genetic diversity of Sappinia-like strains (Amoebozoa, Thecamoebidae) revealed by SSU rRNA investigations. Parasitol Res. 2009;105(3):869-73.
Wylezich, C., Walochnik, J., & Michel, R. (2009). High genetic diversity of Sappinia-like strains (Amoebozoa, Thecamoebidae) revealed by SSU rRNA investigations. Parasitology Research, 105(3), 869-73. https://doi.org/10.1007/s00436-009-1482-1
Wylezich C, Walochnik J, Michel R. High Genetic Diversity of Sappinia-like Strains (Amoebozoa, Thecamoebidae) Revealed By SSU rRNA Investigations. Parasitol Res. 2009;105(3):869-73. PubMed PMID: 19495795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High genetic diversity of Sappinia-like strains (Amoebozoa, Thecamoebidae) revealed by SSU rRNA investigations. AU - Wylezich,Claudia, AU - Walochnik,Julia, AU - Michel,Rolf, Y1 - 2009/06/03/ PY - 2009/04/07/received PY - 2009/05/08/accepted PY - 2009/6/5/entrez PY - 2009/6/6/pubmed PY - 2009/9/22/medline SP - 869 EP - 73 JF - Parasitology research JO - Parasitol Res VL - 105 IS - 3 N2 - Sappinia diploidea is known as a free-living amoeba of worldwide distribution and has also been reported as causative agent of a brain infection in an immunocompetent young man. In the current study, we were able to isolate eight strains of S. diploidea-like amoebae identified by light microscopy from different habitats. Cultures of all strains were established successfully for molecular characterization. The small subunit ribosomal RNA genes of all strains were sequenced and compared to one another, to the neotype of S. diploidea, and to strains of Sappinia pedata, the only other Sappinia species known to date, from GenBank by multiple sequence alignment and cluster analysis. Altogether, the phylogenetic position of the genus Sappinia within the Thecamoebidae was corroborated; however, it was shown that the genus splits into several well-separated clusters making the establishment of new species within this genus inevitable. Furthermore, two of the S. diploidea-like strains were actually more closely related to S. pedata than to S. diploidea, although the diagnostically relevant standing form which seems to be characteristic for S. pedata was not observed in either of the two strains. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/19495795/High_genetic_diversity_of_Sappinia_like_strains__Amoebozoa_Thecamoebidae__revealed_by_SSU_rRNA_investigations_ L2 - https://dx.doi.org/10.1007/s00436-009-1482-1 DB - PRIME DP - Unbound Medicine ER -