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Gas-phase fragmentation study of biotin reagents using electrospray ionization tandem mass spectrometry on a quadrupole orthogonal time-of-flight hybrid instrument.
Rapid Commun Mass Spectrom. 2009 Jul; 23(13):1941-56.RC

Abstract

In this study, we evaluated, by electrospray ionization mass spectrometry (ESI-MS) and collision-induced dissociation tandem mass spectrometry (CID-MS/MS) using a quadrupole orthogonal time-of-flight (QqToF)-MS/MS hybrid instrument, the gas-phase fragmentations of some commercially available biotinyl reagents. The biotin reagents used were: psoralen-BPE 1, p-diazobenzoyl biocytin (DBB) 2, photoreactive biotin 3, biotinyl-hexaethyleneglycol dimer 4, and the sulfo-SBED 5. The results showed that, during ESI-MS and CID-MS/MS analyses, the biotin reagents followed a similar gas-phase fragmentation pattern and the cleavages usually occurred at either end of the spacer arm of the biotin reagents. In general we have observed that the CID-MS/MS fragmentation routes of the five precursor protonated molecules obtained from the biotin linkers 1-5 afforded a series of product ions formed essentially by similar routes. The genesis and the structural identities of all the product ions obtained from the biotin linkers 1-5 have been assigned. All the exact mass assignments of the protonated molecules and the product ions were verified by conducting separate CID-MS/MS analysis of the deuterium-labelled precursor ions.

Authors+Show Affiliations

Department of Chemistry, Memorial University of Newfoundland, St. John's, Newfoundland, A1B 3V6, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19496066

Citation

Sioud, Salim, et al. "Gas-phase Fragmentation Study of Biotin Reagents Using Electrospray Ionization Tandem Mass Spectrometry On a Quadrupole Orthogonal Time-of-flight Hybrid Instrument." Rapid Communications in Mass Spectrometry : RCM, vol. 23, no. 13, 2009, pp. 1941-56.
Sioud S, Genestie B, Jahouh F, et al. Gas-phase fragmentation study of biotin reagents using electrospray ionization tandem mass spectrometry on a quadrupole orthogonal time-of-flight hybrid instrument. Rapid Commun Mass Spectrom. 2009;23(13):1941-56.
Sioud, S., Genestie, B., Jahouh, F., Martin, P., & Banoub, J. (2009). Gas-phase fragmentation study of biotin reagents using electrospray ionization tandem mass spectrometry on a quadrupole orthogonal time-of-flight hybrid instrument. Rapid Communications in Mass Spectrometry : RCM, 23(13), 1941-56. https://doi.org/10.1002/rcm.4091
Sioud S, et al. Gas-phase Fragmentation Study of Biotin Reagents Using Electrospray Ionization Tandem Mass Spectrometry On a Quadrupole Orthogonal Time-of-flight Hybrid Instrument. Rapid Commun Mass Spectrom. 2009;23(13):1941-56. PubMed PMID: 19496066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gas-phase fragmentation study of biotin reagents using electrospray ionization tandem mass spectrometry on a quadrupole orthogonal time-of-flight hybrid instrument. AU - Sioud,Salim, AU - Genestie,Benoit, AU - Jahouh,Farid, AU - Martin,Patrick, AU - Banoub,Joseph, PY - 2009/6/5/entrez PY - 2009/6/6/pubmed PY - 2009/8/14/medline SP - 1941 EP - 56 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 13 N2 - In this study, we evaluated, by electrospray ionization mass spectrometry (ESI-MS) and collision-induced dissociation tandem mass spectrometry (CID-MS/MS) using a quadrupole orthogonal time-of-flight (QqToF)-MS/MS hybrid instrument, the gas-phase fragmentations of some commercially available biotinyl reagents. The biotin reagents used were: psoralen-BPE 1, p-diazobenzoyl biocytin (DBB) 2, photoreactive biotin 3, biotinyl-hexaethyleneglycol dimer 4, and the sulfo-SBED 5. The results showed that, during ESI-MS and CID-MS/MS analyses, the biotin reagents followed a similar gas-phase fragmentation pattern and the cleavages usually occurred at either end of the spacer arm of the biotin reagents. In general we have observed that the CID-MS/MS fragmentation routes of the five precursor protonated molecules obtained from the biotin linkers 1-5 afforded a series of product ions formed essentially by similar routes. The genesis and the structural identities of all the product ions obtained from the biotin linkers 1-5 have been assigned. All the exact mass assignments of the protonated molecules and the product ions were verified by conducting separate CID-MS/MS analysis of the deuterium-labelled precursor ions. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/19496066/Gas_phase_fragmentation_study_of_biotin_reagents_using_electrospray_ionization_tandem_mass_spectrometry_on_a_quadrupole_orthogonal_time_of_flight_hybrid_instrument_ L2 - https://doi.org/10.1002/rcm.4091 DB - PRIME DP - Unbound Medicine ER -