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[Expression of inflammatory factors in lung tissue of acute paraquat poisoned rats].

Abstract

OBJECTIVE

To investigate the expression of inflammatory factors in lung tissue of acute paraquat (PQ) poisoned rats.

METHODS

Fifty male SD rats were randomized divided into two groups: the normal control group (NC group, n = 10) and the PQ group (n = 40). The 1 ml saline was administered once in normal control group. The PQ group was administered with 20 mg/kg 1% PQ by intraperitoneal injection to establish the model of PQ induced lung injury. At six hours, at the first, the third and the seventh day the PQ group were sacrificed, while at the first day the normal control group was sacrificed. The level of tumor necrosis factor alpha (TNF-alpha) mRNA, interleukin 10 (IL-10) mRNA, high mobility group box 1 (HMGB-1) mRNA in lung of rats were detected. Meanwhile, pathological changes of the lung were examined under optical microscope.

RESULTS

Compared with that in normal control group, TNF-alpha mRNA expression in lung tissue of PQ group reached the peak at the six hour and decreased slowly at the first day [(0.740 +/- 0.100) and (0.584 +/- 0.049) respectively]. At the six hour and the first day in PQ group it was significantly higher than that in normal control group (P < 0.05 or P < 0.01). IL-10 mRNA expression in lung tissue of PQ group was elevated at the six hour, reached the peak at the first day, at the third day [(0.551 +/- 0.016) and (0.524 +/- 0.010) respectively] and the seventh day also higher than that in normal control group. At the first and the seventh day in the PQ group it was significantly higher than that in normal control group (P < 0.01). Meanwhile, HMGB-1 mRNA expression in lung tissue of PQ group was also elevated at the six hour, reached the peak at the first day, at the third [(0.695 +/- 0.060), (0.871 +/- 0.154) and (0.819 +/- 0.188) respectively] and the seventh day also higher than that in normal control group. At six hour, the first and the third day in the PQ group it was significantly higher than that in normal control group (P < 0.01). The histological changes such as alveolar edema, hemorrhage and inflammatory cell infiltration in the PQ group were more than those in the normal control group.

CONCLUSION

In rats after PQ intoxication the levels of the inflammatory factors TNF-alpha, IL-10 and HMGB-1 are higher than normal rats, and inflammatory could play an important role in lung injury of poisoned rats.

Authors+Show Affiliations

Emergercy Medical Department, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19497220

Citation

He, Xiao-yan, et al. "[Expression of Inflammatory Factors in Lung Tissue of Acute Paraquat Poisoned Rats]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 27, no. 3, 2009, pp. 149-52.
He XY, Sun Q, Li ZW, et al. [Expression of inflammatory factors in lung tissue of acute paraquat poisoned rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009;27(3):149-52.
He, X. Y., Sun, Q., Li, Z. W., Lu, Z. Q., Hong, G. L., Liang, H., Qiu, Q. M., & Hu, G. X. (2009). [Expression of inflammatory factors in lung tissue of acute paraquat poisoned rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 27(3), 149-52.
He XY, et al. [Expression of Inflammatory Factors in Lung Tissue of Acute Paraquat Poisoned Rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009;27(3):149-52. PubMed PMID: 19497220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of inflammatory factors in lung tissue of acute paraquat poisoned rats]. AU - He,Xiao-yan, AU - Sun,Qi, AU - Li,Zhong-wang, AU - Lu,Zhong-qiu, AU - Hong,Guang-liang, AU - Liang,Huan, AU - Qiu,Qiao-meng, AU - Hu,Guo-xin, PY - 2009/6/6/entrez PY - 2009/6/6/pubmed PY - 2012/7/20/medline SP - 149 EP - 52 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 27 IS - 3 N2 - OBJECTIVE: To investigate the expression of inflammatory factors in lung tissue of acute paraquat (PQ) poisoned rats. METHODS: Fifty male SD rats were randomized divided into two groups: the normal control group (NC group, n = 10) and the PQ group (n = 40). The 1 ml saline was administered once in normal control group. The PQ group was administered with 20 mg/kg 1% PQ by intraperitoneal injection to establish the model of PQ induced lung injury. At six hours, at the first, the third and the seventh day the PQ group were sacrificed, while at the first day the normal control group was sacrificed. The level of tumor necrosis factor alpha (TNF-alpha) mRNA, interleukin 10 (IL-10) mRNA, high mobility group box 1 (HMGB-1) mRNA in lung of rats were detected. Meanwhile, pathological changes of the lung were examined under optical microscope. RESULTS: Compared with that in normal control group, TNF-alpha mRNA expression in lung tissue of PQ group reached the peak at the six hour and decreased slowly at the first day [(0.740 +/- 0.100) and (0.584 +/- 0.049) respectively]. At the six hour and the first day in PQ group it was significantly higher than that in normal control group (P < 0.05 or P < 0.01). IL-10 mRNA expression in lung tissue of PQ group was elevated at the six hour, reached the peak at the first day, at the third day [(0.551 +/- 0.016) and (0.524 +/- 0.010) respectively] and the seventh day also higher than that in normal control group. At the first and the seventh day in the PQ group it was significantly higher than that in normal control group (P < 0.01). Meanwhile, HMGB-1 mRNA expression in lung tissue of PQ group was also elevated at the six hour, reached the peak at the first day, at the third [(0.695 +/- 0.060), (0.871 +/- 0.154) and (0.819 +/- 0.188) respectively] and the seventh day also higher than that in normal control group. At six hour, the first and the third day in the PQ group it was significantly higher than that in normal control group (P < 0.01). The histological changes such as alveolar edema, hemorrhage and inflammatory cell infiltration in the PQ group were more than those in the normal control group. CONCLUSION: In rats after PQ intoxication the levels of the inflammatory factors TNF-alpha, IL-10 and HMGB-1 are higher than normal rats, and inflammatory could play an important role in lung injury of poisoned rats. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/19497220/[Expression_of_inflammatory_factors_in_lung_tissue_of_acute_paraquat_poisoned_rats]_ DB - PRIME DP - Unbound Medicine ER -