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Automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay.
Anal Chem. 2009 Jul 01; 81(13):5484-9.AC

Abstract

An automated support-resolution strategy was designed to couple with a flow-through immunosensing system for performing a one-way chemiluminescent (CL) multiplex immunoassay. Different from multilabel and multichannel-based detection techniques, this immunoassay method employed a single horseradish peroxidase (HRP) label in one way. With the use of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as model analytes, the capture antibodies for CEA and AFP were immobilized on the inner wall of a glass tube and the surface of paramagnetic microspheres (PMs), respectively. The on-line incubation could be performed in the glass tube after introducing the mixture of CEA, HRP-labeled anti-CEA antibody, AFP, anti-AFP immobilized PMs, and HRP-labeled anti-AFP antibody. With the use of a wash step, the formed sandwich immunocomplexes were separated automatically and the immunocomplex immobilized PMs were captured in another unmodified glass tube with a magnet. The CL signals from the two glass tubes were near-simultaneously collected with the aid of an optical shutter to perform quantitative detection. CEA and AFP could be rapidly assayed in the ranges of 1.0-60 and 1.0-80 ng/mL within 27 min. The assay results of clinical serum samples with the proposed method were in an acceptable agreement with the reference values. This system provides a promising multiplex immunoassay approach for clinical applications.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19499927

Citation

Yang, Zhanjun, et al. "Automated Support-resolution Strategy for a One-way Chemiluminescent Multiplex Immunoassay." Analytical Chemistry, vol. 81, no. 13, 2009, pp. 5484-9.
Yang Z, Liu H, Zong C, et al. Automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay. Anal Chem. 2009;81(13):5484-9.
Yang, Z., Liu, H., Zong, C., Yan, F., & Ju, H. (2009). Automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay. Analytical Chemistry, 81(13), 5484-9. https://doi.org/10.1021/ac900724m
Yang Z, et al. Automated Support-resolution Strategy for a One-way Chemiluminescent Multiplex Immunoassay. Anal Chem. 2009 Jul 1;81(13):5484-9. PubMed PMID: 19499927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay. AU - Yang,Zhanjun, AU - Liu,Hong, AU - Zong,Chen, AU - Yan,Feng, AU - Ju,Huangxian, PY - 2009/6/9/entrez PY - 2009/6/9/pubmed PY - 2009/9/30/medline SP - 5484 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 13 N2 - An automated support-resolution strategy was designed to couple with a flow-through immunosensing system for performing a one-way chemiluminescent (CL) multiplex immunoassay. Different from multilabel and multichannel-based detection techniques, this immunoassay method employed a single horseradish peroxidase (HRP) label in one way. With the use of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as model analytes, the capture antibodies for CEA and AFP were immobilized on the inner wall of a glass tube and the surface of paramagnetic microspheres (PMs), respectively. The on-line incubation could be performed in the glass tube after introducing the mixture of CEA, HRP-labeled anti-CEA antibody, AFP, anti-AFP immobilized PMs, and HRP-labeled anti-AFP antibody. With the use of a wash step, the formed sandwich immunocomplexes were separated automatically and the immunocomplex immobilized PMs were captured in another unmodified glass tube with a magnet. The CL signals from the two glass tubes were near-simultaneously collected with the aid of an optical shutter to perform quantitative detection. CEA and AFP could be rapidly assayed in the ranges of 1.0-60 and 1.0-80 ng/mL within 27 min. The assay results of clinical serum samples with the proposed method were in an acceptable agreement with the reference values. This system provides a promising multiplex immunoassay approach for clinical applications. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19499927/Automated_support_resolution_strategy_for_a_one_way_chemiluminescent_multiplex_immunoassay_ L2 - https://doi.org/10.1021/ac900724m DB - PRIME DP - Unbound Medicine ER -