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A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry.
J Chromatogr A. 2009 Jul 10; 1216(28):5398-415.JC

Abstract

In the present study, a methodology based on liquid chromatography with diode array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface and a triple quadrupole mass spectrometer for the simultaneous identification of phenolic compounds in fruit juices has been developed. 72 available phenolic compound standards from diverse families present in fruits have been studied in order to analyze their fragmentation pattern. As a result, a general strategy for the characterization of unknown phenolic compounds in fruit juices was designed: (i) taking into account its UV-visible spectrum and elution order, assign the unknown polyphenol to a polyphenol class, (ii) identify the quasi-molecular ion using positive and negative MS spectra, being supported by adducts generated with solvent or sodium and molecular complexes, (iii) determinate the pattern of glycosylation in positive mode using ESI(+)-CID MS/MS product ion scan experiments, selecting the quasi-molecular ion as precursor ion, and finally, (iv) study the identity of the aglycone through ESI(+)-CID MS/MS product ion spectra from the protonated aglycone, [Y(0)](+). This strategy was successfully employed for the characterization of known and unknown phenolic compounds in juices from 17 different fruits.

Authors+Show Affiliations

Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, P.O. Box 644, 48080 Bilbao, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19500791

Citation

Abad-García, Beatriz, et al. "A General Analytical Strategy for the Characterization of Phenolic Compounds in Fruit Juices By High-performance Liquid Chromatography With Diode Array Detection Coupled to Electrospray Ionization and Triple Quadrupole Mass Spectrometry." Journal of Chromatography. A, vol. 1216, no. 28, 2009, pp. 5398-415.
Abad-García B, Berrueta LA, Garmón-Lobato S, et al. A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry. J Chromatogr A. 2009;1216(28):5398-415.
Abad-García, B., Berrueta, L. A., Garmón-Lobato, S., Gallo, B., & Vicente, F. (2009). A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry. Journal of Chromatography. A, 1216(28), 5398-415. https://doi.org/10.1016/j.chroma.2009.05.039
Abad-García B, et al. A General Analytical Strategy for the Characterization of Phenolic Compounds in Fruit Juices By High-performance Liquid Chromatography With Diode Array Detection Coupled to Electrospray Ionization and Triple Quadrupole Mass Spectrometry. J Chromatogr A. 2009 Jul 10;1216(28):5398-415. PubMed PMID: 19500791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A general analytical strategy for the characterization of phenolic compounds in fruit juices by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry. AU - Abad-García,Beatriz, AU - Berrueta,Luis A, AU - Garmón-Lobato,Sergio, AU - Gallo,Blanca, AU - Vicente,Francisca, Y1 - 2009/05/21/ PY - 2009/02/17/received PY - 2009/05/07/revised PY - 2009/05/14/accepted PY - 2009/6/9/entrez PY - 2009/6/9/pubmed PY - 2009/9/15/medline SP - 5398 EP - 415 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 28 N2 - In the present study, a methodology based on liquid chromatography with diode array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface and a triple quadrupole mass spectrometer for the simultaneous identification of phenolic compounds in fruit juices has been developed. 72 available phenolic compound standards from diverse families present in fruits have been studied in order to analyze their fragmentation pattern. As a result, a general strategy for the characterization of unknown phenolic compounds in fruit juices was designed: (i) taking into account its UV-visible spectrum and elution order, assign the unknown polyphenol to a polyphenol class, (ii) identify the quasi-molecular ion using positive and negative MS spectra, being supported by adducts generated with solvent or sodium and molecular complexes, (iii) determinate the pattern of glycosylation in positive mode using ESI(+)-CID MS/MS product ion scan experiments, selecting the quasi-molecular ion as precursor ion, and finally, (iv) study the identity of the aglycone through ESI(+)-CID MS/MS product ion spectra from the protonated aglycone, [Y(0)](+). This strategy was successfully employed for the characterization of known and unknown phenolic compounds in juices from 17 different fruits. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19500791/A_general_analytical_strategy_for_the_characterization_of_phenolic_compounds_in_fruit_juices_by_high_performance_liquid_chromatography_with_diode_array_detection_coupled_to_electrospray_ionization_and_triple_quadrupole_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00776-6 DB - PRIME DP - Unbound Medicine ER -