Tags

Type your tag names separated by a space and hit enter

Xanthine oxidase/laponite nanoparticles immobilized on glassy carbon electrode: direct electron transfer and multielectrocatalysis.
Biosens Bioelectron. 2009 Aug 15; 24(12):3556-61.BB

Abstract

In this work, colloidal laponite nanoparticles were further expanded into the design of the third-generation biosensor. Direct electrochemistry of the complex molybdoenzyme xanthine oxidase (XnOx) immobilized on glassy carbon electrode (GCE) by laponite nanoparticles was investigated for the first time. XnOx/laponite thin film modified electrode showed only one pair of well defined and reversible cyclic voltammetric peaks attributed to XnOx-FAD cofactor at about -0.370 V vs. SCE (pH 5). The formal potential of XnOx-FAD/FADH(2) couple varied linearly with the increase of pH in the range of 4.0-8.0 with a slope of -54.3 mV pH(-1), which indicated that two-proton transfer was accompanied with two-electron transfer in the electrochemical reaction. More interestingly, the immobilized XnOx retained its biological activity well and displayed an excellent electrocatalytic performance to both the oxidation of xanthine and the reduction of nitrate. The electrocatalytic response showed a linear dependence on the xanthine concentration ranging from 3.9 x 10(-8) to 2.1 x 10(-5)M with a detection limit of 1.0 x 10(-8)M based on S/N=3.

Authors+Show Affiliations

College of Chemistry & Chemical Engineering, Yangzhou University, Jiangsu 225002, China. danshan@yzu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19500969

Citation

Shan, Dan, et al. "Xanthine Oxidase/laponite Nanoparticles Immobilized On Glassy Carbon Electrode: Direct Electron Transfer and Multielectrocatalysis." Biosensors & Bioelectronics, vol. 24, no. 12, 2009, pp. 3556-61.
Shan D, Wang YN, Xue HG, et al. Xanthine oxidase/laponite nanoparticles immobilized on glassy carbon electrode: direct electron transfer and multielectrocatalysis. Biosens Bioelectron. 2009;24(12):3556-61.
Shan, D., Wang, Y. N., Xue, H. G., Cosnier, S., & Ding, S. N. (2009). Xanthine oxidase/laponite nanoparticles immobilized on glassy carbon electrode: direct electron transfer and multielectrocatalysis. Biosensors & Bioelectronics, 24(12), 3556-61. https://doi.org/10.1016/j.bios.2009.05.009
Shan D, et al. Xanthine Oxidase/laponite Nanoparticles Immobilized On Glassy Carbon Electrode: Direct Electron Transfer and Multielectrocatalysis. Biosens Bioelectron. 2009 Aug 15;24(12):3556-61. PubMed PMID: 19500969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xanthine oxidase/laponite nanoparticles immobilized on glassy carbon electrode: direct electron transfer and multielectrocatalysis. AU - Shan,Dan, AU - Wang,Yan-Na, AU - Xue,Huai-Guo, AU - Cosnier,Serge, AU - Ding,Shou-Nian, Y1 - 2009/05/18/ PY - 2009/01/21/received PY - 2009/04/17/revised PY - 2009/05/11/accepted PY - 2009/6/9/entrez PY - 2009/6/9/pubmed PY - 2009/10/1/medline SP - 3556 EP - 61 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 12 N2 - In this work, colloidal laponite nanoparticles were further expanded into the design of the third-generation biosensor. Direct electrochemistry of the complex molybdoenzyme xanthine oxidase (XnOx) immobilized on glassy carbon electrode (GCE) by laponite nanoparticles was investigated for the first time. XnOx/laponite thin film modified electrode showed only one pair of well defined and reversible cyclic voltammetric peaks attributed to XnOx-FAD cofactor at about -0.370 V vs. SCE (pH 5). The formal potential of XnOx-FAD/FADH(2) couple varied linearly with the increase of pH in the range of 4.0-8.0 with a slope of -54.3 mV pH(-1), which indicated that two-proton transfer was accompanied with two-electron transfer in the electrochemical reaction. More interestingly, the immobilized XnOx retained its biological activity well and displayed an excellent electrocatalytic performance to both the oxidation of xanthine and the reduction of nitrate. The electrocatalytic response showed a linear dependence on the xanthine concentration ranging from 3.9 x 10(-8) to 2.1 x 10(-5)M with a detection limit of 1.0 x 10(-8)M based on S/N=3. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19500969/Xanthine_oxidase/laponite_nanoparticles_immobilized_on_glassy_carbon_electrode:_direct_electron_transfer_and_multielectrocatalysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00271-1 DB - PRIME DP - Unbound Medicine ER -