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Evaluation of enzymatic biomarkers and lipoperoxidation level in Hediste diversicolor exposed to copper and benzo[a]pyrene.
Ecotoxicol Environ Saf 2009; 72(7):1893-8EE

Abstract

This study aims to evaluate the effects of exposure to copper, benzo[a]pyrene, and to their mixture on enzymatic and lipid peroxidation biomarkers in Hediste diversicolor. Worms were submitted to 1 microM of both single compounds and to their mixture during a period of test of 12, 24, 36, and 48 h. The biomarkers selected in this work were the activities of cytochrome P450-dependent NADPH cytochrome c reductase (NADPH red) as phase I enzyme, glutathione-S-transferase (GST) as phase II enzyme, and the acetylcholinesterase (AChE) activity as neurotoxicity marker. Oxidative stress was evaluated using catalase activity (CAT) and malondialdehyde accumulation (MDA). The NADPH red activity was not significantly affected by copper exposure; it shows a drastic increase in both B[a]P and mixture-exposed organisms. GST activities were significant in B[a]P-exposed worms only after 36 h, and in animals exposed to the mixture after 12 and 48 h. The ACHE activity was inhibited only in B[a]P-exposed worms.

Authors+Show Affiliations

Laboratoire de Biochimie et de Toxicologie Environnementale, Institut Supérieur Agronomique, Chott-Mariem, Sousse, Tunisia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19501399

Citation

Bouraoui, Zied, et al. "Evaluation of Enzymatic Biomarkers and Lipoperoxidation Level in Hediste Diversicolor Exposed to Copper and Benzo[a]pyrene." Ecotoxicology and Environmental Safety, vol. 72, no. 7, 2009, pp. 1893-8.
Bouraoui Z, Banni M, Ghedira J, et al. Evaluation of enzymatic biomarkers and lipoperoxidation level in Hediste diversicolor exposed to copper and benzo[a]pyrene. Ecotoxicol Environ Saf. 2009;72(7):1893-8.
Bouraoui, Z., Banni, M., Ghedira, J., Clerandeau, C., Narbonne, J. F., & Boussetta, H. (2009). Evaluation of enzymatic biomarkers and lipoperoxidation level in Hediste diversicolor exposed to copper and benzo[a]pyrene. Ecotoxicology and Environmental Safety, 72(7), pp. 1893-8. doi:10.1016/j.ecoenv.2009.05.011.
Bouraoui Z, et al. Evaluation of Enzymatic Biomarkers and Lipoperoxidation Level in Hediste Diversicolor Exposed to Copper and Benzo[a]pyrene. Ecotoxicol Environ Saf. 2009;72(7):1893-8. PubMed PMID: 19501399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of enzymatic biomarkers and lipoperoxidation level in Hediste diversicolor exposed to copper and benzo[a]pyrene. AU - Bouraoui,Zied, AU - Banni,Mohamed, AU - Ghedira,Jihen, AU - Clerandeau,Christelle, AU - Narbonne,Jean François, AU - Boussetta,Hamadi, Y1 - 2009/06/06/ PY - 2009/03/01/received PY - 2009/04/29/revised PY - 2009/05/17/accepted PY - 2009/6/9/entrez PY - 2009/6/9/pubmed PY - 2009/12/16/medline SP - 1893 EP - 8 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 72 IS - 7 N2 - This study aims to evaluate the effects of exposure to copper, benzo[a]pyrene, and to their mixture on enzymatic and lipid peroxidation biomarkers in Hediste diversicolor. Worms were submitted to 1 microM of both single compounds and to their mixture during a period of test of 12, 24, 36, and 48 h. The biomarkers selected in this work were the activities of cytochrome P450-dependent NADPH cytochrome c reductase (NADPH red) as phase I enzyme, glutathione-S-transferase (GST) as phase II enzyme, and the acetylcholinesterase (AChE) activity as neurotoxicity marker. Oxidative stress was evaluated using catalase activity (CAT) and malondialdehyde accumulation (MDA). The NADPH red activity was not significantly affected by copper exposure; it shows a drastic increase in both B[a]P and mixture-exposed organisms. GST activities were significant in B[a]P-exposed worms only after 36 h, and in animals exposed to the mixture after 12 and 48 h. The ACHE activity was inhibited only in B[a]P-exposed worms. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/19501399/Evaluation_of_enzymatic_biomarkers_and_lipoperoxidation_level_in_Hediste_diversicolor_exposed_to_copper_and_benzo[a]pyrene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(09)00126-2 DB - PRIME DP - Unbound Medicine ER -