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Lung toxicity induced by intratracheal instillation of size-fractionated tire particles.
Toxicol Lett. 2009 Sep 28; 189(3):206-14.TL

Abstract

Tire particles (TP) represent a significant component of urban air pollution (PM), constituting more than 10% of PM10 mass at urban locations with heavy traffic. The purpose of this study was to evaluate the effects of size-fractionated TP in an animal exposure model frequently used to assess the health effects of air pollutants. Potential pro-inflammatory and toxic effects of TP2.5 (<2.5 microm) and TP10 (<10 microm) were investigated through instillation of suspensions of these materials in BALB/c mice. Bronchoalveolar lavage fluid (BALF) was screened for total protein, lactate dehydrogenase (LDH), alkaline phosphatase (AP), and beta-glucuronidase (B-Gluc) as markers of cytotoxicity; glutathione (GSH) and superoxide dismutase (SOD) as markers of oxidative potential; and tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-2 (MIP-2), and inflammatory cells as markers of inflammation. Concomitantly, histological analysis of TP-exposed lungs was performed. A single intratracheal instillation of 10 microg/100 microl, 100 microg/100 microl or 200 microg/100 microl was performed, and after 24h mice were euthanized and BALF examined. Inflammatory cellular profiles showed dose-dependent responses after TP10 exposure, while strong cytotoxic effects, including increases in total protein, LDH and AP, were observed to be associated to TP2.5 exposure. Histologically, TP10-treated lungs mainly showed inflammatory tissue infiltration, in contrast to TP2.5-treated lungs, where lysis of the alveolar barrier appeared to be the most characteristic lesion. Our biochemical, cytological, and histological results indicated differential lung toxicity mechanisms elicited by size-fractionated TP, in agreement with other studies performed in in vivo systems that have shown that lung responses to inhaled or instilled particles are affected by particle size. We conclude that lung toxicity induced by TP10 was primarily due to macrophage-mediated inflammatory events, while toxicity induced by TP2.5 appeared to be related more closely to cytotoxicity.

Authors+Show Affiliations

Department of Environmental Sciences, POLARIS Research Center, University of Milano-Bicocca, 1 piazza della Scienza, Milan 20126, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19501637

Citation

Mantecca, Paride, et al. "Lung Toxicity Induced By Intratracheal Instillation of Size-fractionated Tire Particles." Toxicology Letters, vol. 189, no. 3, 2009, pp. 206-14.
Mantecca P, Sancini G, Moschini E, et al. Lung toxicity induced by intratracheal instillation of size-fractionated tire particles. Toxicol Lett. 2009;189(3):206-14.
Mantecca, P., Sancini, G., Moschini, E., Farina, F., Gualtieri, M., Rohr, A., Miserocchi, G., Palestini, P., & Camatini, M. (2009). Lung toxicity induced by intratracheal instillation of size-fractionated tire particles. Toxicology Letters, 189(3), 206-14. https://doi.org/10.1016/j.toxlet.2009.05.023
Mantecca P, et al. Lung Toxicity Induced By Intratracheal Instillation of Size-fractionated Tire Particles. Toxicol Lett. 2009 Sep 28;189(3):206-14. PubMed PMID: 19501637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lung toxicity induced by intratracheal instillation of size-fractionated tire particles. AU - Mantecca,Paride, AU - Sancini,Giulio, AU - Moschini,Elisa, AU - Farina,Francesca, AU - Gualtieri,Maurizio, AU - Rohr,Annette, AU - Miserocchi,Giuseppe, AU - Palestini,Paola, AU - Camatini,Marina, Y1 - 2009/06/06/ PY - 2009/02/10/received PY - 2009/05/22/revised PY - 2009/05/27/accepted PY - 2009/6/9/entrez PY - 2009/6/9/pubmed PY - 2009/7/31/medline SP - 206 EP - 14 JF - Toxicology letters JO - Toxicol Lett VL - 189 IS - 3 N2 - Tire particles (TP) represent a significant component of urban air pollution (PM), constituting more than 10% of PM10 mass at urban locations with heavy traffic. The purpose of this study was to evaluate the effects of size-fractionated TP in an animal exposure model frequently used to assess the health effects of air pollutants. Potential pro-inflammatory and toxic effects of TP2.5 (<2.5 microm) and TP10 (<10 microm) were investigated through instillation of suspensions of these materials in BALB/c mice. Bronchoalveolar lavage fluid (BALF) was screened for total protein, lactate dehydrogenase (LDH), alkaline phosphatase (AP), and beta-glucuronidase (B-Gluc) as markers of cytotoxicity; glutathione (GSH) and superoxide dismutase (SOD) as markers of oxidative potential; and tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-2 (MIP-2), and inflammatory cells as markers of inflammation. Concomitantly, histological analysis of TP-exposed lungs was performed. A single intratracheal instillation of 10 microg/100 microl, 100 microg/100 microl or 200 microg/100 microl was performed, and after 24h mice were euthanized and BALF examined. Inflammatory cellular profiles showed dose-dependent responses after TP10 exposure, while strong cytotoxic effects, including increases in total protein, LDH and AP, were observed to be associated to TP2.5 exposure. Histologically, TP10-treated lungs mainly showed inflammatory tissue infiltration, in contrast to TP2.5-treated lungs, where lysis of the alveolar barrier appeared to be the most characteristic lesion. Our biochemical, cytological, and histological results indicated differential lung toxicity mechanisms elicited by size-fractionated TP, in agreement with other studies performed in in vivo systems that have shown that lung responses to inhaled or instilled particles are affected by particle size. We conclude that lung toxicity induced by TP10 was primarily due to macrophage-mediated inflammatory events, while toxicity induced by TP2.5 appeared to be related more closely to cytotoxicity. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/19501637/Lung_toxicity_induced_by_intratracheal_instillation_of_size_fractionated_tire_particles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(09)00271-9 DB - PRIME DP - Unbound Medicine ER -