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Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats.
J Ethnopharmacol 2009; 124(1):142-50JE

Abstract

BACKGROUND/AIMS

Hepatic fibrosis is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. TCM 319 recipe is a Chinese Medicine formula which consists of six Chinese herbs. In this study, we investigated the anti-fibrotic efficacy and mechanisms of TCM 319 recipe.

METHODS

Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl4). 34 male adult SD rats were allocated into five groups (group 1-concomitant CCl4 and TCM 319 recipe for 8 weeks; group 2-CCl4 for 4 weeks and then CCl4 and TCM 319 recipe for 4 weeks; group 3-CCl4 alone for 8 weeks; group 4-TCM 319 recipe only for 8 weeks; group 5-untreated controls). After 8 weeks of treatment, serum ALT assay, liver tissue histological examination and immunostaining were carried out to examine the liver function and fibrosis degree. The expression levels of platelet derived growth factor (PDGF-B), PDGF-Rbeta, and transforming growth factor-beta 1 (TGF-beta1) were measured by quantitative RT-PCR and western blot.

RESULTS

TCM 319 recipe reduced liver injury and attenuated hepatic fibrosis in group 1 compared with that in group 3. TCM 319 recipe suppressed the mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1). In addition, treatment with TCM 319 recipe significantly down-regulated mRNA expression of PDGF-B and PDGF-Rbeta, and it also suppressed protein expression of PDGF-Rbeta and TGF-beta1.

CONCLUSIONS

TCM 319 recipe extracts could attenuate hepatic fibrosis induced by CCl4 in rats. The anti-fibrotic effect of TCM 319 recipe is associated with the down-regulation of mRNA expression of TIMP-1, PDGF-B and PDGF-Rbeta, and with the suppression of protein expression of PDGF-Rbeta and TGF-beta1.

Authors+Show Affiliations

Department of Medicine, The University of Hong Kong, HKSAR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19501992

Citation

Cheung, K F., et al. "Therapeutic Efficacy of Traditional Chinese Medicine 319 Recipe On Hepatic Fibrosis Induced By Carbon Tetrachloride in Rats." Journal of Ethnopharmacology, vol. 124, no. 1, 2009, pp. 142-50.
Cheung KF, Ye DW, Yang ZF, et al. Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats. J Ethnopharmacol. 2009;124(1):142-50.
Cheung, K. F., Ye, D. W., Yang, Z. F., Lu, L., Liu, C. H., Wang, X. L., ... Lau, G. K. (2009). Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats. Journal of Ethnopharmacology, 124(1), pp. 142-50. doi:10.1016/j.jep.2009.03.005.
Cheung KF, et al. Therapeutic Efficacy of Traditional Chinese Medicine 319 Recipe On Hepatic Fibrosis Induced By Carbon Tetrachloride in Rats. J Ethnopharmacol. 2009 Jul 6;124(1):142-50. PubMed PMID: 19501992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats. AU - Cheung,K F, AU - Ye,D W, AU - Yang,Z F, AU - Lu,L, AU - Liu,C H, AU - Wang,X L, AU - Poon,R T P, AU - Tong,Y, AU - Liu,P, AU - Chen,Y C, AU - Lau,George K K, Y1 - 2009/03/20/ PY - 2008/09/25/received PY - 2009/03/02/revised PY - 2009/03/07/accepted PY - 2009/6/9/entrez PY - 2009/6/9/pubmed PY - 2009/9/10/medline SP - 142 EP - 50 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 124 IS - 1 N2 - BACKGROUND/AIMS: Hepatic fibrosis is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. TCM 319 recipe is a Chinese Medicine formula which consists of six Chinese herbs. In this study, we investigated the anti-fibrotic efficacy and mechanisms of TCM 319 recipe. METHODS: Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl4). 34 male adult SD rats were allocated into five groups (group 1-concomitant CCl4 and TCM 319 recipe for 8 weeks; group 2-CCl4 for 4 weeks and then CCl4 and TCM 319 recipe for 4 weeks; group 3-CCl4 alone for 8 weeks; group 4-TCM 319 recipe only for 8 weeks; group 5-untreated controls). After 8 weeks of treatment, serum ALT assay, liver tissue histological examination and immunostaining were carried out to examine the liver function and fibrosis degree. The expression levels of platelet derived growth factor (PDGF-B), PDGF-Rbeta, and transforming growth factor-beta 1 (TGF-beta1) were measured by quantitative RT-PCR and western blot. RESULTS: TCM 319 recipe reduced liver injury and attenuated hepatic fibrosis in group 1 compared with that in group 3. TCM 319 recipe suppressed the mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1). In addition, treatment with TCM 319 recipe significantly down-regulated mRNA expression of PDGF-B and PDGF-Rbeta, and it also suppressed protein expression of PDGF-Rbeta and TGF-beta1. CONCLUSIONS: TCM 319 recipe extracts could attenuate hepatic fibrosis induced by CCl4 in rats. The anti-fibrotic effect of TCM 319 recipe is associated with the down-regulation of mRNA expression of TIMP-1, PDGF-B and PDGF-Rbeta, and with the suppression of protein expression of PDGF-Rbeta and TGF-beta1. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19501992/Therapeutic_efficacy_of_Traditional_Chinese_Medicine_319_recipe_on_hepatic_fibrosis_induced_by_carbon_tetrachloride_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00144-5 DB - PRIME DP - Unbound Medicine ER -