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Diagnosis of histoplasmosis in bronchoalveolar lavage fluid by intracytoplasmic localization of silver-positive yeast.
Acta Cytol. 1991 Nov-Dec; 35(6):710-2.AC

Abstract

A case of histoplasmosis in a patient with acquired immunodeficiency syndrome is presented. Intracytoplasmic organisms were found in Diff-Quik- and Papanicolaou-stained bronchoalveolar lavage fluid sediment. Budding yeasts were found in the silver methenamine-stained material. It was initially thought that the yeasts might represent another infecting organism, especially in view of the patient's history of esophageal candidiasis. When the silver-stained material was counterstained with hematoxylin and eosin, simultaneous demonstration of cytologic detail and silver positivity showed that the silver-positive yeasts were intracellular, confirming that they were Histoplasma capsulatum. Counterstaining with hematoxylin and eosin allows localization of argyrophilic organisms to either intracellular or extracellular sites and is technically simple to perform. It is useful in acquired immunodeficiency syndrome patients and other immunocompromised hosts, in whom multiple organisms are often found.

Authors+Show Affiliations

Department of Pathology, University of California-San Francisco.No affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

1950319

Citation

Blumenfeld, W, and G L. Gan. "Diagnosis of Histoplasmosis in Bronchoalveolar Lavage Fluid By Intracytoplasmic Localization of Silver-positive Yeast." Acta Cytologica, vol. 35, no. 6, 1991, pp. 710-2.
Blumenfeld W, Gan GL. Diagnosis of histoplasmosis in bronchoalveolar lavage fluid by intracytoplasmic localization of silver-positive yeast. Acta Cytol. 1991;35(6):710-2.
Blumenfeld, W., & Gan, G. L. (1991). Diagnosis of histoplasmosis in bronchoalveolar lavage fluid by intracytoplasmic localization of silver-positive yeast. Acta Cytologica, 35(6), 710-2.
Blumenfeld W, Gan GL. Diagnosis of Histoplasmosis in Bronchoalveolar Lavage Fluid By Intracytoplasmic Localization of Silver-positive Yeast. Acta Cytol. 1991 Nov-Dec;35(6):710-2. PubMed PMID: 1950319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of histoplasmosis in bronchoalveolar lavage fluid by intracytoplasmic localization of silver-positive yeast. AU - Blumenfeld,W, AU - Gan,G L, PY - 1991/11/1/pubmed PY - 1991/11/1/medline PY - 1991/11/1/entrez SP - 710 EP - 2 JF - Acta cytologica JO - Acta Cytol VL - 35 IS - 6 N2 - A case of histoplasmosis in a patient with acquired immunodeficiency syndrome is presented. Intracytoplasmic organisms were found in Diff-Quik- and Papanicolaou-stained bronchoalveolar lavage fluid sediment. Budding yeasts were found in the silver methenamine-stained material. It was initially thought that the yeasts might represent another infecting organism, especially in view of the patient's history of esophageal candidiasis. When the silver-stained material was counterstained with hematoxylin and eosin, simultaneous demonstration of cytologic detail and silver positivity showed that the silver-positive yeasts were intracellular, confirming that they were Histoplasma capsulatum. Counterstaining with hematoxylin and eosin allows localization of argyrophilic organisms to either intracellular or extracellular sites and is technically simple to perform. It is useful in acquired immunodeficiency syndrome patients and other immunocompromised hosts, in whom multiple organisms are often found. SN - 0001-5547 UR - https://www.unboundmedicine.com/medline/citation/1950319/Diagnosis_of_histoplasmosis_in_bronchoalveolar_lavage_fluid_by_intracytoplasmic_localization_of_silver_positive_yeast_ L2 - https://medlineplus.gov/histoplasmosis.html DB - PRIME DP - Unbound Medicine ER -