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Erdosteine and ebselen as useful agents in intestinal ischemia/reperfusion injury.
J Surg Res. 2009 Aug; 155(2):210-6.JS

Abstract

BACKGROUND

Reactive oxygen and nitrogen species generated during reperfusion of the tissue are characteristic of ischemia/reperfusion (I/R) injury. The purpose of the present study was to investigate whether erdosteine and ebselen, molecules with antioxidant properties and peroxynitrite scavenging capability, respectively, can reduce oxidative stress and histological damage in the rat small bowel subjected to mesenteric I/R injury.

MATERIALS AND METHODS

Forty Sprague-Dawley rats were divided into five groups equally: sham, I/R, I/R plus erdosteine, I/R plus ebselen, and I/R plus erdosteine and ebselen. Intestinal ischemia for 45 min and reperfusion for 3 d were carried out. Ileal specimens were obtained to determine the tissue levels of malondialdehide (MDA), protein carbonyl content (PCC), superoxide dismutase (SOD), glutathione peroxidase (GPx), nitrite/nitrate (NO(x)) level and histological changes.

RESULTS

Intestinal I/R resulted in increased tissue MDA, PCC, and NO(x) levels and decreased SOD and GPx activities. Both erdosteine and ebselen alone significantly decreased MDA, PCC, and NO(x) levels and increased antioxidant enzymes activities, but all values were different from control. These changes almost returned to control values in the group treated with erdostein and ebselen. Histopathologically, the intestinal injury in rats treated with erdosteine and ebselen as well as combination were less than I/R group.

CONCLUSIONS

Both erdosteine and ebselen were able to attenuate I/R injury of the intestine via inhibition of lipid peroxidation and protein oxidation, maintenance of antioxidant, and free radical scavenger properties. Nevertheless, combination treatment showed more promising results, suggesting that scavenging peroxynitrite nearby antioxidant activity is important in preventing intestinal I/R injury.

Authors+Show Affiliations

Department of Pediatrics, Gulhane Military Medical Academy, Etlik, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19505699

Citation

Tunc, Turan, et al. "Erdosteine and Ebselen as Useful Agents in Intestinal Ischemia/reperfusion Injury." The Journal of Surgical Research, vol. 155, no. 2, 2009, pp. 210-6.
Tunc T, Uysal B, Atabek C, et al. Erdosteine and ebselen as useful agents in intestinal ischemia/reperfusion injury. J Surg Res. 2009;155(2):210-6.
Tunc, T., Uysal, B., Atabek, C., Kesik, V., Caliskan, B., Oztas, E., Ersoz, N., Oter, S., & Guven, A. (2009). Erdosteine and ebselen as useful agents in intestinal ischemia/reperfusion injury. The Journal of Surgical Research, 155(2), 210-6. https://doi.org/10.1016/j.jss.2008.06.017
Tunc T, et al. Erdosteine and Ebselen as Useful Agents in Intestinal Ischemia/reperfusion Injury. J Surg Res. 2009;155(2):210-6. PubMed PMID: 19505699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Erdosteine and ebselen as useful agents in intestinal ischemia/reperfusion injury. AU - Tunc,Turan, AU - Uysal,Bulent, AU - Atabek,Cuneyt, AU - Kesik,Vural, AU - Caliskan,Bahadir, AU - Oztas,Emin, AU - Ersoz,Nail, AU - Oter,Sukru, AU - Guven,Ahmet, Y1 - 2008/07/15/ PY - 2007/12/27/received PY - 2008/03/07/revised PY - 2008/06/13/accepted PY - 2009/6/10/entrez PY - 2009/6/10/pubmed PY - 2009/8/15/medline SP - 210 EP - 6 JF - The Journal of surgical research JO - J Surg Res VL - 155 IS - 2 N2 - BACKGROUND: Reactive oxygen and nitrogen species generated during reperfusion of the tissue are characteristic of ischemia/reperfusion (I/R) injury. The purpose of the present study was to investigate whether erdosteine and ebselen, molecules with antioxidant properties and peroxynitrite scavenging capability, respectively, can reduce oxidative stress and histological damage in the rat small bowel subjected to mesenteric I/R injury. MATERIALS AND METHODS: Forty Sprague-Dawley rats were divided into five groups equally: sham, I/R, I/R plus erdosteine, I/R plus ebselen, and I/R plus erdosteine and ebselen. Intestinal ischemia for 45 min and reperfusion for 3 d were carried out. Ileal specimens were obtained to determine the tissue levels of malondialdehide (MDA), protein carbonyl content (PCC), superoxide dismutase (SOD), glutathione peroxidase (GPx), nitrite/nitrate (NO(x)) level and histological changes. RESULTS: Intestinal I/R resulted in increased tissue MDA, PCC, and NO(x) levels and decreased SOD and GPx activities. Both erdosteine and ebselen alone significantly decreased MDA, PCC, and NO(x) levels and increased antioxidant enzymes activities, but all values were different from control. These changes almost returned to control values in the group treated with erdostein and ebselen. Histopathologically, the intestinal injury in rats treated with erdosteine and ebselen as well as combination were less than I/R group. CONCLUSIONS: Both erdosteine and ebselen were able to attenuate I/R injury of the intestine via inhibition of lipid peroxidation and protein oxidation, maintenance of antioxidant, and free radical scavenger properties. Nevertheless, combination treatment showed more promising results, suggesting that scavenging peroxynitrite nearby antioxidant activity is important in preventing intestinal I/R injury. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/19505699/Erdosteine_and_ebselen_as_useful_agents_in_intestinal_ischemia/reperfusion_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(08)00409-5 DB - PRIME DP - Unbound Medicine ER -