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Circulating endothelial cells, endothelial apoptosis and soluble markers of endothelial dysfunction in patients with systemic lupus erythematosus-related vasculitis.
Int Angiol. 2009 Jun; 28(3):192-201.IA

Abstract

AIM

Evidence is accumulating for endothelial cell dysfunction as one of the main factors initiating vessel wall damage in SLE. Enhanced expression of endothelial adhesion molecules is suggested to play a crucial role in the pathogenesis of vasculitis, while the number of circulating endothelial cells (CECs) is believed to be a reliable marker of endothelial damage. It therefore seems relevant to investigate CECs counts and soluble markers of endothelial dysfunction in SLE patients with inflammatory microangiopathy. The aim of this study was to assess the number of CECs, including apoptotic CECs, as well as to determine serum levels of sVCAM-1, sICAM-1 and sE-selectin in patients with SLE-related vasculitis.

METHODS

The study included 51 women with SLE, divided into 2 subgroups: I patients with severe disease activity according to SLEDAI score, developing vascular complications, such as central nervous system affection and/or vasculitis and/or glomerulonephritis, II patients with mild or moderate disease activity, without vascular complications. The control group consisted of 16 healthy female volunteers. CECs, including apoptotic CECs, were isolated using anti-CD146-coated immunomagnetic Dynabeads. Serum levels of sVCAM-1, sICAM-1 and sE-selectin levels were determined with ELISA.

RESULTS

In patients with SLE, CEC counts were significantly higher than in healthy controls, and strongly correlated with disease activity assessed by SLEDAI score. The number of apoptotic CECs, as compared with healthy subjects, increased considerably only in subgroup I. Serum sVCAM-1 levels were notably increased in subgroup I in relation to subgroup II and in subgroup II in relation to the control group, while serum sICAM-1 and sE-selectin levels in both subgroups were comparable and significantly higher than those of healthy subjects.

CONCLUSIONS

The study showed that the number of CECs increases in SLE and strongly correlates with disease activity, reaching maximum values at the stage of inflammatory microangiopathy-related complications. Severe SLE flares are characterized by enhanced endothelial cell apoptosis. Progressive increase in serum sVCAM-1 levels is connected with disease activity aggravation and development of lupus microangiopathy.

Authors+Show Affiliations

Department of Angiology, Arterial Hypertension and Diabetology, Wroclaw Medical University, Wroclaw, Poland. joannakluz@wp.plNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19506538

Citation

Kluz, J, et al. "Circulating Endothelial Cells, Endothelial Apoptosis and Soluble Markers of Endothelial Dysfunction in Patients With Systemic Lupus Erythematosus-related Vasculitis." International Angiology : a Journal of the International Union of Angiology, vol. 28, no. 3, 2009, pp. 192-201.
Kluz J, Kopeć W, Jakobsche-Policht U, et al. Circulating endothelial cells, endothelial apoptosis and soluble markers of endothelial dysfunction in patients with systemic lupus erythematosus-related vasculitis. Int Angiol. 2009;28(3):192-201.
Kluz, J., Kopeć, W., Jakobsche-Policht, U., & Adamiec, R. (2009). Circulating endothelial cells, endothelial apoptosis and soluble markers of endothelial dysfunction in patients with systemic lupus erythematosus-related vasculitis. International Angiology : a Journal of the International Union of Angiology, 28(3), 192-201.
Kluz J, et al. Circulating Endothelial Cells, Endothelial Apoptosis and Soluble Markers of Endothelial Dysfunction in Patients With Systemic Lupus Erythematosus-related Vasculitis. Int Angiol. 2009;28(3):192-201. PubMed PMID: 19506538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating endothelial cells, endothelial apoptosis and soluble markers of endothelial dysfunction in patients with systemic lupus erythematosus-related vasculitis. AU - Kluz,J, AU - Kopeć,W, AU - Jakobsche-Policht,U, AU - Adamiec,R, PY - 2009/6/10/entrez PY - 2009/6/10/pubmed PY - 2010/10/13/medline SP - 192 EP - 201 JF - International angiology : a journal of the International Union of Angiology JO - Int Angiol VL - 28 IS - 3 N2 - AIM: Evidence is accumulating for endothelial cell dysfunction as one of the main factors initiating vessel wall damage in SLE. Enhanced expression of endothelial adhesion molecules is suggested to play a crucial role in the pathogenesis of vasculitis, while the number of circulating endothelial cells (CECs) is believed to be a reliable marker of endothelial damage. It therefore seems relevant to investigate CECs counts and soluble markers of endothelial dysfunction in SLE patients with inflammatory microangiopathy. The aim of this study was to assess the number of CECs, including apoptotic CECs, as well as to determine serum levels of sVCAM-1, sICAM-1 and sE-selectin in patients with SLE-related vasculitis. METHODS: The study included 51 women with SLE, divided into 2 subgroups: I patients with severe disease activity according to SLEDAI score, developing vascular complications, such as central nervous system affection and/or vasculitis and/or glomerulonephritis, II patients with mild or moderate disease activity, without vascular complications. The control group consisted of 16 healthy female volunteers. CECs, including apoptotic CECs, were isolated using anti-CD146-coated immunomagnetic Dynabeads. Serum levels of sVCAM-1, sICAM-1 and sE-selectin levels were determined with ELISA. RESULTS: In patients with SLE, CEC counts were significantly higher than in healthy controls, and strongly correlated with disease activity assessed by SLEDAI score. The number of apoptotic CECs, as compared with healthy subjects, increased considerably only in subgroup I. Serum sVCAM-1 levels were notably increased in subgroup I in relation to subgroup II and in subgroup II in relation to the control group, while serum sICAM-1 and sE-selectin levels in both subgroups were comparable and significantly higher than those of healthy subjects. CONCLUSIONS: The study showed that the number of CECs increases in SLE and strongly correlates with disease activity, reaching maximum values at the stage of inflammatory microangiopathy-related complications. Severe SLE flares are characterized by enhanced endothelial cell apoptosis. Progressive increase in serum sVCAM-1 levels is connected with disease activity aggravation and development of lupus microangiopathy. SN - 0392-9590 UR - https://www.unboundmedicine.com/medline/citation/19506538/Circulating_endothelial_cells_endothelial_apoptosis_and_soluble_markers_of_endothelial_dysfunction_in_patients_with_systemic_lupus_erythematosus_related_vasculitis_ L2 - http://www.minervamedica.it/index2.t?show=R34Y2009N03A0192 DB - PRIME DP - Unbound Medicine ER -