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Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells.
Toxicol In Vitro. 2009 Sep; 23(6):1076-84.TV

Abstract

Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells. Toxicity induced by silver (Ag(+)) ions was studied in parallel using AgNO(3) as the Ag(+) ion source. Using cation exchange treatment, we confirmed that the AgNP solution contained a negligible amount of free Ag(+) ions. Metal-responsive metallothionein 1b (MT1b) mRNA expression was not induced in AgNP-treated cells, while it was induced in AgNO(3)-treated cells. These results indicate that AgNP-treated cells have limited exposure to Ag(+) ions, despite the potential release of Ag(+) ions from AgNPs in cell culture. AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and induced intracellular oxidative stress. AgNPs exhibited cytotoxicity with a potency comparable to that of Ag(+) ions in in vitro cytotoxicity assays. However, the toxicity of AgNPs was prevented by use of the antioxidant N-acetylcysteine, and AgNP-induced DNA damage was also prevented by N-acetylcysteine. AgNO(3) treatment induced oxidative stress-related glutathione peroxidase 1 (GPx1) and catalase expression to a greater extent than AgNP exposure, but treatment with AgNO(3) and AgNPs induced comparable superoxide dismutase 1 (SOD1) expression levels. Our findings suggest that AgNP cytotoxicity is primarily the result of oxidative stress and is independent of the toxicity of Ag(+) ions.

Authors+Show Affiliations

College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19508889

Citation

Kim, Soohee, et al. "Oxidative Stress-dependent Toxicity of Silver Nanoparticles in Human Hepatoma Cells." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 23, no. 6, 2009, pp. 1076-84.
Kim S, Choi JE, Choi J, et al. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol In Vitro. 2009;23(6):1076-84.
Kim, S., Choi, J. E., Choi, J., Chung, K. H., Park, K., Yi, J., & Ryu, D. Y. (2009). Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 23(6), 1076-84. https://doi.org/10.1016/j.tiv.2009.06.001
Kim S, et al. Oxidative Stress-dependent Toxicity of Silver Nanoparticles in Human Hepatoma Cells. Toxicol In Vitro. 2009;23(6):1076-84. PubMed PMID: 19508889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. AU - Kim,Soohee, AU - Choi,Ji Eun, AU - Choi,Jinhee, AU - Chung,Kyu-Hyuck, AU - Park,Kwangsik, AU - Yi,Jongheop, AU - Ryu,Doug-Young, Y1 - 2009/06/07/ PY - 2009/03/05/received PY - 2009/05/04/revised PY - 2009/06/02/accepted PY - 2009/6/11/entrez PY - 2009/6/11/pubmed PY - 2009/12/16/medline SP - 1076 EP - 84 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 23 IS - 6 N2 - Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells. Toxicity induced by silver (Ag(+)) ions was studied in parallel using AgNO(3) as the Ag(+) ion source. Using cation exchange treatment, we confirmed that the AgNP solution contained a negligible amount of free Ag(+) ions. Metal-responsive metallothionein 1b (MT1b) mRNA expression was not induced in AgNP-treated cells, while it was induced in AgNO(3)-treated cells. These results indicate that AgNP-treated cells have limited exposure to Ag(+) ions, despite the potential release of Ag(+) ions from AgNPs in cell culture. AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and induced intracellular oxidative stress. AgNPs exhibited cytotoxicity with a potency comparable to that of Ag(+) ions in in vitro cytotoxicity assays. However, the toxicity of AgNPs was prevented by use of the antioxidant N-acetylcysteine, and AgNP-induced DNA damage was also prevented by N-acetylcysteine. AgNO(3) treatment induced oxidative stress-related glutathione peroxidase 1 (GPx1) and catalase expression to a greater extent than AgNP exposure, but treatment with AgNO(3) and AgNPs induced comparable superoxide dismutase 1 (SOD1) expression levels. Our findings suggest that AgNP cytotoxicity is primarily the result of oxidative stress and is independent of the toxicity of Ag(+) ions. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/19508889/Oxidative_stress_dependent_toxicity_of_silver_nanoparticles_in_human_hepatoma_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(09)00129-5 DB - PRIME DP - Unbound Medicine ER -