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Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells.
Am J Clin Nutr 2009; 90(2):415-24AJ

Abstract

BACKGROUND

Polyunsaturated fatty acids can have beneficial effects on human immune cells, such as peripheral blood mononuclear cells (PBMCs). However, the mechanisms of action of polyunsaturated fatty acids on immune cells are still largely unknown.

OBJECTIVE

The objective was to examine the effects of supplementation with the polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on whole-genome PBMC gene expression profiles, in healthy Dutch elderly subjects participating in a double-blind trial, by using whole-genome transcriptomics analysis.

DESIGN

The subjects were randomly allocated to 1 of 3 groups: 1) consumption of 1.8 g EPA+DHA/d (n = 36), 2) consumption of 0.4 g EPA+DHA/d (n = 37), or 3) consumption of 4.0 g high-oleic acid sunflower oil (HOSF)/d (n = 38). All supplements were given in capsules. Before and after 26 wk of intervention, blood samples were collected. Microarray analysis was performed on PBMC RNA from 23 subjects who received 1.8 g EPA+DHA/d and 25 subjects who received HOSF capsules. Quantitative real-time polymerase chain reaction was performed in all 111 subjects.

RESULTS

A high EPA+DHA intake changed the expression of 1040 genes, whereas HOSF intake changed the expression of only 298 genes. EPA+DHA intake resulted in a decreased expression of genes involved in inflammatory- and atherogenic-related pathways, such as nuclear transcription factor kappaB signaling, eicosanoid synthesis, scavenger receptor activity, adipogenesis, and hypoxia signaling.

CONCLUSION

These results are the first to show that intake of EPA+DHA for 26 wk can alter the gene expression profiles of PBMCs to a more antiinflammatory and antiatherogenic status. This trial was registered at clinicaltrials.gov as NCT00124852.

Authors+Show Affiliations

Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, 6703 HD Wageningen, Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19515734

Citation

Bouwens, Mark, et al. "Fish-oil Supplementation Induces Antiinflammatory Gene Expression Profiles in Human Blood Mononuclear Cells." The American Journal of Clinical Nutrition, vol. 90, no. 2, 2009, pp. 415-24.
Bouwens M, van de Rest O, Dellschaft N, et al. Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells. Am J Clin Nutr. 2009;90(2):415-24.
Bouwens, M., van de Rest, O., Dellschaft, N., Bromhaar, M. G., de Groot, L. C., Geleijnse, J. M., ... Afman, L. A. (2009). Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells. The American Journal of Clinical Nutrition, 90(2), pp. 415-24. doi:10.3945/ajcn.2009.27680.
Bouwens M, et al. Fish-oil Supplementation Induces Antiinflammatory Gene Expression Profiles in Human Blood Mononuclear Cells. Am J Clin Nutr. 2009;90(2):415-24. PubMed PMID: 19515734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells. AU - Bouwens,Mark, AU - van de Rest,Ondine, AU - Dellschaft,Neele, AU - Bromhaar,Mechteld Grootte, AU - de Groot,Lisette C P G M, AU - Geleijnse,Johanna M, AU - Müller,Michael, AU - Afman,Lydia A, Y1 - 2009/06/10/ PY - 2009/6/12/entrez PY - 2009/6/12/pubmed PY - 2009/8/4/medline SP - 415 EP - 24 JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. VL - 90 IS - 2 N2 - BACKGROUND: Polyunsaturated fatty acids can have beneficial effects on human immune cells, such as peripheral blood mononuclear cells (PBMCs). However, the mechanisms of action of polyunsaturated fatty acids on immune cells are still largely unknown. OBJECTIVE: The objective was to examine the effects of supplementation with the polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on whole-genome PBMC gene expression profiles, in healthy Dutch elderly subjects participating in a double-blind trial, by using whole-genome transcriptomics analysis. DESIGN: The subjects were randomly allocated to 1 of 3 groups: 1) consumption of 1.8 g EPA+DHA/d (n = 36), 2) consumption of 0.4 g EPA+DHA/d (n = 37), or 3) consumption of 4.0 g high-oleic acid sunflower oil (HOSF)/d (n = 38). All supplements were given in capsules. Before and after 26 wk of intervention, blood samples were collected. Microarray analysis was performed on PBMC RNA from 23 subjects who received 1.8 g EPA+DHA/d and 25 subjects who received HOSF capsules. Quantitative real-time polymerase chain reaction was performed in all 111 subjects. RESULTS: A high EPA+DHA intake changed the expression of 1040 genes, whereas HOSF intake changed the expression of only 298 genes. EPA+DHA intake resulted in a decreased expression of genes involved in inflammatory- and atherogenic-related pathways, such as nuclear transcription factor kappaB signaling, eicosanoid synthesis, scavenger receptor activity, adipogenesis, and hypoxia signaling. CONCLUSION: These results are the first to show that intake of EPA+DHA for 26 wk can alter the gene expression profiles of PBMCs to a more antiinflammatory and antiatherogenic status. This trial was registered at clinicaltrials.gov as NCT00124852. SN - 1938-3207 UR - https://www.unboundmedicine.com/medline/citation/19515734/Fish_oil_supplementation_induces_antiinflammatory_gene_expression_profiles_in_human_blood_mononuclear_cells_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.3945/ajcn.2009.27680 DB - PRIME DP - Unbound Medicine ER -