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Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats.
J Hypertens. 2009 Apr; 27(4):791-9.JH

Abstract

OBJECTIVES

To investigate whether hypertension could modify the function of adrenergic, nitrergic, and sensory innervations involved in the electrical field stimulation-induced response in mesenteric arteries from female rats.

METHODS

Vascular reactivity experiments were performed in endothelium-denuded mesenteric arteries from normotensive, Wistar-Kyoto and spontaneously hypertensive female rats; protein expression was measured by western blot; nitric oxide release was measured by fluorometry; calcitonin gene-related peptide and noradrenaline release were determined by enzyme immunoassay.

RESULTS

The electrical field stimulation-induced contractions were significantly lower in segments from spontaneously hypertensive rats than those of Wistar-Kyoto rats. Hypertension did not modify either the response or release of noradrenaline. Preincubation with the nitric oxide synthase inhibitor N(G)-nitro-L-arginine methyl ester increased the electrical field stimulation-induced contractions only in segments from Wistar-Kyoto rats. The relaxation induced by the nitric oxide donor sodium nitroprusside was similar in segments from both strains. The electrical field stimulation-induced nitric oxide release was decreased in segments from spontaneously hypertensive rats. The calcitonin gene-related peptide receptor antagonist CGRP(8-37) did not alter the electrical field stimulation-induced contractions in segments from Wistar-Kyoto rats but increased them in segments from spontaneously hypertensive rats. The calcitonin gene-related peptide-induced relaxation was increased in segments from spontaneously hypertensive rats. The expression of the 15-kDa active form of RAMP1 was increased in segments from spontaneously hypertensive rats.

CONCLUSION

In contrast to male rats, electrical field stimulation-induced contractions are decreased in hypertensive female rats. Nitrergic innervation plays a role in the development and/or maintenance of hypertension, whereas sensory innervation is a counteracting mechanism through the increased calcitonin gene-related peptide response.

Authors+Show Affiliations

Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19516178

Citation

del Campo, Lara, et al. "Hypertension Alters the Function of Nitrergic and Sensory Innervation in Mesenteric Arteries From Female Rats." Journal of Hypertension, vol. 27, no. 4, 2009, pp. 791-9.
del Campo L, Ferrer M, Balfagón G. Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats. J Hypertens. 2009;27(4):791-9.
del Campo, L., Ferrer, M., & Balfagón, G. (2009). Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats. Journal of Hypertension, 27(4), 791-9. https://doi.org/10.1097/HJH.0b013e32832531e6
del Campo L, Ferrer M, Balfagón G. Hypertension Alters the Function of Nitrergic and Sensory Innervation in Mesenteric Arteries From Female Rats. J Hypertens. 2009;27(4):791-9. PubMed PMID: 19516178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypertension alters the function of nitrergic and sensory innervation in mesenteric arteries from female rats. AU - del Campo,Lara, AU - Ferrer,Mercedes, AU - Balfagón,Gloria, PY - 2009/6/12/entrez PY - 2009/6/12/pubmed PY - 2009/8/27/medline SP - 791 EP - 9 JF - Journal of hypertension JO - J Hypertens VL - 27 IS - 4 N2 - OBJECTIVES: To investigate whether hypertension could modify the function of adrenergic, nitrergic, and sensory innervations involved in the electrical field stimulation-induced response in mesenteric arteries from female rats. METHODS: Vascular reactivity experiments were performed in endothelium-denuded mesenteric arteries from normotensive, Wistar-Kyoto and spontaneously hypertensive female rats; protein expression was measured by western blot; nitric oxide release was measured by fluorometry; calcitonin gene-related peptide and noradrenaline release were determined by enzyme immunoassay. RESULTS: The electrical field stimulation-induced contractions were significantly lower in segments from spontaneously hypertensive rats than those of Wistar-Kyoto rats. Hypertension did not modify either the response or release of noradrenaline. Preincubation with the nitric oxide synthase inhibitor N(G)-nitro-L-arginine methyl ester increased the electrical field stimulation-induced contractions only in segments from Wistar-Kyoto rats. The relaxation induced by the nitric oxide donor sodium nitroprusside was similar in segments from both strains. The electrical field stimulation-induced nitric oxide release was decreased in segments from spontaneously hypertensive rats. The calcitonin gene-related peptide receptor antagonist CGRP(8-37) did not alter the electrical field stimulation-induced contractions in segments from Wistar-Kyoto rats but increased them in segments from spontaneously hypertensive rats. The calcitonin gene-related peptide-induced relaxation was increased in segments from spontaneously hypertensive rats. The expression of the 15-kDa active form of RAMP1 was increased in segments from spontaneously hypertensive rats. CONCLUSION: In contrast to male rats, electrical field stimulation-induced contractions are decreased in hypertensive female rats. Nitrergic innervation plays a role in the development and/or maintenance of hypertension, whereas sensory innervation is a counteracting mechanism through the increased calcitonin gene-related peptide response. SN - 1473-5598 UR - https://www.unboundmedicine.com/medline/citation/19516178/Hypertension_alters_the_function_of_nitrergic_and_sensory_innervation_in_mesenteric_arteries_from_female_rats_ L2 - https://doi.org/10.1097/HJH.0b013e32832531e6 DB - PRIME DP - Unbound Medicine ER -