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Light-emitting-diode-induced chemiluminescence detection for capillary electrophoresis.
Electrophoresis. 2009 Jun; 30(11):1937-42.E

Abstract

In this work, an LED-induced-chemiluminescence (LED-CL) system was developed to extend the application of CL detection in CE. In the LED-CL, the analyte photooxidizes luminol under the irradiation of LEDs and generates CL. Taking the advantage of the small size nature of LEDs, the constructed photoreactor is greatly miniaturized, and especially suitable as a CE detector. The feasibility of the proposed detector was evaluated by detection of riboflavin (RF), flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) after CE separation. Under the optimized conditions, the LODs for RF, FMN and FAD were 0.007, 0.02 and 0.1 microg/mL, respectively, better than those by UV detection. The RSDs were 3.4, 3.6 and 4.1% for 0.5 microg/mL RF, 2 microg/mL FMN and 5 microg/mL FAD, respectively. The LED-CL detector features low cost, miniaturization, fast response, high sensitivity and good reproducibility.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang X
College of Chemistry, Sichuan University, Chengdu, Sichuan, P. R. China.
Zhang J
No affiliation info available
Wu X
No affiliation info available
Lv Y
No affiliation info available
Hou X
No affiliation info available

MeSH

Electrophoresis, CapillaryEquipment DesignFlavinsHydrogen-Ion ConcentrationLightLinear ModelsLuminescent MeasurementsLuminolPhotochemical ProcessesReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19517444

Citation

Zhang, Xinfeng, et al. "Light-emitting-diode-induced Chemiluminescence Detection for Capillary Electrophoresis." Electrophoresis, vol. 30, no. 11, 2009, pp. 1937-42.
Zhang X, Zhang J, Wu X, et al. Light-emitting-diode-induced chemiluminescence detection for capillary electrophoresis. Electrophoresis. 2009;30(11):1937-42.
Zhang, X., Zhang, J., Wu, X., Lv, Y., & Hou, X. (2009). Light-emitting-diode-induced chemiluminescence detection for capillary electrophoresis. Electrophoresis, 30(11), 1937-42. https://doi.org/10.1002/elps.200800708
Zhang X, et al. Light-emitting-diode-induced Chemiluminescence Detection for Capillary Electrophoresis. Electrophoresis. 2009;30(11):1937-42. PubMed PMID: 19517444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Light-emitting-diode-induced chemiluminescence detection for capillary electrophoresis. AU - Zhang,Xinfeng, AU - Zhang,Jiyou, AU - Wu,Xi, AU - Lv,Yi, AU - Hou,Xiandeng, PY - 2009/6/12/entrez PY - 2009/6/12/pubmed PY - 2009/9/17/medline SP - 1937 EP - 42 JF - Electrophoresis JO - Electrophoresis VL - 30 IS - 11 N2 - In this work, an LED-induced-chemiluminescence (LED-CL) system was developed to extend the application of CL detection in CE. In the LED-CL, the analyte photooxidizes luminol under the irradiation of LEDs and generates CL. Taking the advantage of the small size nature of LEDs, the constructed photoreactor is greatly miniaturized, and especially suitable as a CE detector. The feasibility of the proposed detector was evaluated by detection of riboflavin (RF), flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) after CE separation. Under the optimized conditions, the LODs for RF, FMN and FAD were 0.007, 0.02 and 0.1 microg/mL, respectively, better than those by UV detection. The RSDs were 3.4, 3.6 and 4.1% for 0.5 microg/mL RF, 2 microg/mL FMN and 5 microg/mL FAD, respectively. The LED-CL detector features low cost, miniaturization, fast response, high sensitivity and good reproducibility. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/19517444/Light_emitting_diode_induced_chemiluminescence_detection_for_capillary_electrophoresis_ L2 - https://doi.org/10.1002/elps.200800708 DB - PRIME DP - Unbound Medicine ER -