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Acteoside protects human neuroblastoma SH-SY5Y cells against beta-amyloid-induced cell injury.
Brain Res. 2009 Aug 04; 1283:139-47.BR

Abstract

Amyloid beta-peptide (Abeta) has been implicated in the pathogenesis of AD. It can cause cell death in AD by evoking a cascade of oxidative damage to neurons. So antioxidant compounds may throw a light on the treatment of AD. In the present study, we investigated the protective effect of acteoside (AS), an antioxidative phenylethanoid glycoside, on Abeta(25-35)-induced SH-SY5Y cell injury. Exposure of cells to 25 muM Abeta(25-35) for 24 h caused viability loss, apoptotic increase and reactive oxygen species (ROS) increase, pre-treatment with acteoside for 1.5 h significantly reduced the viability loss, apoptotic rate and attenuated Abeta-mediated ROS production. In addition, AS strikingly inhibited Abeta(25-35)-induced mitochondrial dysfunctions, including lowered membrane potential, increased Bax/Bcl-2 ratio, cytochrome c release and the cleavage of caspase-3. Taken together, these results indicated that acteoside could protect SH-SY5Y cells against beta-amyloid-induced cell injury by the attenuating ROS production and the modulating apoptotic signal pathway through Bcl-2 family, cytochrome c, and caspase-3.

Authors+Show Affiliations

Department of Neurobiology, State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19520063

Citation

Wang, Hongquan, et al. "Acteoside Protects Human Neuroblastoma SH-SY5Y Cells Against Beta-amyloid-induced Cell Injury." Brain Research, vol. 1283, 2009, pp. 139-47.
Wang H, Xu Y, Yan J, et al. Acteoside protects human neuroblastoma SH-SY5Y cells against beta-amyloid-induced cell injury. Brain Res. 2009;1283:139-47.
Wang, H., Xu, Y., Yan, J., Zhao, X., Sun, X., Zhang, Y., Guo, J., & Zhu, C. (2009). Acteoside protects human neuroblastoma SH-SY5Y cells against beta-amyloid-induced cell injury. Brain Research, 1283, 139-47. https://doi.org/10.1016/j.brainres.2009.05.101
Wang H, et al. Acteoside Protects Human Neuroblastoma SH-SY5Y Cells Against Beta-amyloid-induced Cell Injury. Brain Res. 2009 Aug 4;1283:139-47. PubMed PMID: 19520063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acteoside protects human neuroblastoma SH-SY5Y cells against beta-amyloid-induced cell injury. AU - Wang,Hongquan, AU - Xu,Yuxia, AU - Yan,Jie, AU - Zhao,Xiaoyan, AU - Sun,Xiaobo, AU - Zhang,Yanping, AU - Guo,Jingchun, AU - Zhu,Cuiqing, Y1 - 2009/06/09/ PY - 2009/04/27/received PY - 2009/05/24/revised PY - 2009/05/27/accepted PY - 2009/6/13/entrez PY - 2009/6/13/pubmed PY - 2009/10/24/medline SP - 139 EP - 47 JF - Brain research JO - Brain Res VL - 1283 N2 - Amyloid beta-peptide (Abeta) has been implicated in the pathogenesis of AD. It can cause cell death in AD by evoking a cascade of oxidative damage to neurons. So antioxidant compounds may throw a light on the treatment of AD. In the present study, we investigated the protective effect of acteoside (AS), an antioxidative phenylethanoid glycoside, on Abeta(25-35)-induced SH-SY5Y cell injury. Exposure of cells to 25 muM Abeta(25-35) for 24 h caused viability loss, apoptotic increase and reactive oxygen species (ROS) increase, pre-treatment with acteoside for 1.5 h significantly reduced the viability loss, apoptotic rate and attenuated Abeta-mediated ROS production. In addition, AS strikingly inhibited Abeta(25-35)-induced mitochondrial dysfunctions, including lowered membrane potential, increased Bax/Bcl-2 ratio, cytochrome c release and the cleavage of caspase-3. Taken together, these results indicated that acteoside could protect SH-SY5Y cells against beta-amyloid-induced cell injury by the attenuating ROS production and the modulating apoptotic signal pathway through Bcl-2 family, cytochrome c, and caspase-3. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/19520063/Acteoside_protects_human_neuroblastoma_SH_SY5Y_cells_against_beta_amyloid_induced_cell_injury_ DB - PRIME DP - Unbound Medicine ER -